Association of KMT2C Genetic Variants with the Clinicopathologic Development of Oral Cancer

Abstract

Lysine methyltransferase 2C (KMT2C) is a tumor-suppressor gene in several myeloid cells and epithelia and is linked with blood and solid tumor cancers. KMT2C single-nucleotide polymorphisms (SNPs) are also connected with several cancer types. Our study aimed to explore the potential genetic polymorphisms of KMT2C in oral cancer. Five KMT2C SNPs, including rs201834857, rs4725443, rs6464221, rs74483926, and rs6943984, were evaluated in 284 cancer-free controls and 284 oral squamous cell carcinoma (OSCC) cases. We found that individuals with the TC genotype or TC + CC genotype of rs4725443 had a higher risk of oral cancer incidence than those with the TT genotype. Further analysis of KMT2C SNP rs4725443 revealed that the TC + CC genotype of rs4725443 was associated with a significantly advanced tumor stage in the non-alcohol-drinking population. Moreover, the TC + CC genotype of rs4725443 was connected with poor cell differentiation in the alcohol-drinking population. Through analyzing a dataset from The Cancer Genome Atlas (TCGA), we found that reduced KMT2C levels were associated with advanced tumor stage, lymph node invasion, and poor cell differentiation in head and neck squamous cell carcinomas. Our data suggest that KMT2C SNP rs4725443 is a potential genetic marker for oral cancer patients in both non-alcohol-drinking and alcohol-drinking populations.

LDL Receptor-Related Protein 1B Polymorphisms Associated with Increased Risk of Lymph Node Metastasis in Oral Cancer Group with Diabetes Mellitus

“…We used IBM SPSS Statistics v22.0 (IBM, Armonk, NY, USA) to perform analyses in our study similar to previous papers [32]. First, the demographic and laboratory data between the non-OSCC group and the OSCC group were shown using descriptive analysis including mean, standard deviation (SD), and percentage, and evaluated using the exact Mann-Whitney U test difference between the two groups.…”

“…The probe IDs for TaqMan-SNP Genotyping Assay Data Sheets were C_29842934 (rs10496915), C_822593 (rs431809), and C_2115669 (rs6742944), and all probes were stored at −20 • C. In each TaqMan-MGB genotyping mix, one primer matched perfectly to the wild-type sequence variant labeled with VIC, while the second primer matched to the mutant (SNP) sequence variant labeled with 6-carboxyfluorescein (FAM) [30]. Similarly, in our previous research, we used DNA extraction, preservation, and analysis techniques [31,32]. EDTA-containing sterile tubes containing whole blood samples were collected from patients and immediately centrifuged and stored at −80 • C. The genomic DNA was extracted from peripheral blood leukocytes using a QIAamp DNA blood mini kit according to the manufacturer's protocol (Qiagen, Valencia, CA, USA), and then dissolved in TE buffer and stored at −20 • C. Optical density was quantified based on 260 nm wavelength measurements.…”

The Interaction between CLSPN Gene Polymorphisms and Alcohol Consumption Contributes to Oral Cancer Progression

“…We used IBM SPSS Statistics v22.0 (IBM, Armonk, NY, USA) to conduct the analyses in our study, similarly to previous papers [51]. First, the demographic and laboratory data between the non-OSCC group and the OSCC group were shown using descriptive analysis including mean, standard deviation (SD) and percentage, and evaluated using a Mann-Whitney U test to test the difference between the two groups.…”

“…Similar to our previous research, we used DNA extraction, preservation, and analysis techniques [43,51]. Whole blood samples were collected from patients into sterile tubes containing EDTA, which were immediately centrifuged and stored at −80 • C. The genomic DNA was extracted from peripheral blood leukocytes using a QIAamp DNA blood mini kit, and then dissolved in TE buffer and stored at −20 • C. Quantification was based on measuring the optical density at a wavelength of 260 nm.…”