Association of laboratory parameters and genetic polymorphisms with ischemic stroke in Chinese Han population

Abstract

Numerous genetic polymorphisms and clinical laboratory parameters are associated with ischemic stroke (IS). However, the results of such studies have frequently been inconsistent. The aim of the present study was to evaluate associations between clinical laboratory parameters with genetic polymorphisms that influence the risk of IS in a Chinese Han population. Clinical laboratory parameters were measured by an automatic biochemical analyzer. Genotype and allele frequencies of the polymorphisms angiotensin-converting enzyme (ACE) D/I, methylene tetrahydrofolate reductase (MTHFR) C677T and β-fibrinogen (β-Fg) A/G, 455/148T/C were characterized by restriction fragment length polymorphism-PCR. Furthermore, the gene polymorphisms plasminogen activator inhibitor (PAI)-1-4G/5G and apolipoprotein E (ApoE) ε2,3,4 were characterized by allele-specific PCR. The associations of genotype and allele frequencies of the six risk genes in different groups with clinical laboratory parameters were analyzed by chi-square tests. The distribution maps of the polymorphisms of the six genes and clinical laboratory parameters were compared between a control group of 336 healthy individuals and 762 patients with IS. Certain laboratory parameters were associated with ACE I/D, β-Fg-455 A/G and PAI-1 4G/5G. The D allele of ACE I/D was associated with high levels of total cholesterol and low-density lipoprotein cholesterol (LDL-C). Furthermore, high levels of fasting blood glucose, triglyceride and LDL-C were risk factors for IS. There were significant differences in the genotype frequencies of ACE I/D, β-Fg-455 A/G and β-Fg-148 T/C between the IS and the control group. In conclusion, clinical laboratory parameters were associated with the risk of polymorphisms of IS-related genes. The present results support the determination of a range of control values of clinical laboratory parameters for common genotypes in patients with diabetes and hyperlipidemia as a strategy for the early prevention of IS.

Angiotensin-converting enzyme gene insertion/deletion polymorphism and risk of ischemic stroke complication among patients with hypertension in the Ethiopian population

“…Ischemic stroke (IS) is the loss of brain tissue caused by a cessation of blood supply to a region of the brain brought on by an obstruction of a carotid or vertebral artery, or distal branches of the anterior, middle, or posterior cerebral arteries ( 1 ). It is the primary cause of adult disability and the second-leading cause of mortality in the world, accounting for ~ 10% of all deaths with 5.5 million people dying annually ( 2 ).…”

The Impact of ACE Gene Variants on Acute-Phase Reactants in Children with Rheumatic Heart Disease

“…Moreover, the I/D polymorphism was reported to show sexual dimorphism in young adults, with the DD genotype associated with higher maximal fat oxidation values than the ID/II genotype in men, and the ID/II genotype having higher maximal fat oxidation values in women [43]. In ischemic stroke patients, the ACE D allele was associated with high total cholesterol and low-density lipoprotein levels, indicating a possible biomarker for early prevention [44]. In cardiovascular disease patients, the ACE I/I genotype and I allele were associated with increased circulating glucose levels and hence a 2-fold increased risk of diabetes [45].…”

Relationship between fasting blood glucose and subsequent vascular events in Chinese patients with mild ischaemic stroke: a cohort study

“…3 In addition, high triglyceride and low-density lipoprotein-cholesterol (LDL-C) concentrations are risk factors for IS. 4 FBG has also been reported to be significantly associated with the prognosis of IS. It is associated with 90-day mortality and a poor functional outcome 3 months after acute ischaemic stroke (AIS) in patients that are treated by mechanical thrombectomy.…”

“… 3 In addition, high triglyceride and low-density lipoprotein-cholesterol (LDL-C) concentrations are risk factors for IS. 4 …”