Association of Lactobacillus crispatus with fructo-oligosaccharides and ascorbic acid in hydroxypropyl methylcellulose vaginal insert

Vitali, Beatrice; Abruzzo, Angela; Parolin, Carola; Palomino, Rogers Alberto Ñahui; Dalena, Francesco; Bigucci, Federica; Cerchiara, Teresa; Luppi, Barbara

doi:10.1016/j.carbpol.2015.10.035

Cited by 27 publications

(25 citation statements)

References 40 publications

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“…This behavior can be attributed to the peptidoglycan layer of L. brevis CD2, made up of a series of sugars, which can physically interact with HPMC, thus reducing its ability to hydrate. The ability of sugars to limit HPMC hydration was in agreement with other observations reported in the literature [37,38]. Figure 2 shows the in vitro water-uptake results, obtained at pH 6.8 on unloaded HPMC films and probiotic-loaded films.…”

Section: Scanning Electron Microscopy (Sem)supporting

confidence: 89%

“…In this study, HPMC-loaded films showed good mucoadhesive ability as a consequence of interpenetration and entanglement of polymer chains into the mucus layer, as well as the hydrogen bonds established between the polymer hydrophilic groups and the hydrophilic groups of mucus [39,42]. The highest residence time and mucoadhesive ability were obtained when the loaded face of the film was attached to the mucosa: the presence of sugars in the L. brevis CD2 peptidoglycan layer may encourage the formation of other hydrogen and van der Waals bonds, thus increasing the film residence time on porcine mucosa and improving the mucoadhesive properties [37]. Finally, a prolonged release of viable lattobacilli was obtained when the film was attached by exposing the loaded face towards the release medium.…”

Section: Discussionmentioning

confidence: 99%

“…These results were in agreement with our previous published data [39], where a similar residence time was observed for HPMC-based films. Moreover, the presence of sugars in L. brevis CD2 peptidoglycan can contribute to improving the mucoadhesive properties and consequently to increasing the residence time [37]. Mucoadhesive ability was measured with an adapted tensiometer and results showed that the detachment forces were 60 ± 1 × 10 −5 N and 50 ± 2 × 10 −5 N, when the loaded and unloaded face of the film were exposed to the mucosa, respectively.…”

Section: In Vitro Residence Time and Mucoadhesive Ability Of Buccal Fmentioning

confidence: 99%

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Mucoadhesive Buccal Films for Local Delivery of Lactobacillus brevis

et al. 2020

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The aim of this work was to prepare mucoadhesive buccal films for local release of Lactobacillus brevis CD2, which shows interesting anti-inflammatory properties due to its high levels of arginine deiminase. Hydroxypropylmethylcellulose-based films were prepared by means of a modified casting method, which allowed L. brevis CD2 loading on one side of the film, before its complete drying. Three batches of films were prepared, stored at +2-8 • C and +23-25 • C for 48 weeks and characterized in terms of physico-chemical and functional properties. For each batch, the L. brevis viable count and arginine deiminase activity were evaluated at different time points in order to assess functional property maintenance over time. Moreover, the mucoadhesive properties and ability of the films to release L. brevis CD2 were evaluated. A good survival of L. brevis CD2 was observed, particularly at the storage temperature of +2-8 • C, while the activity of arginine deiminase was maintained at both temperature values. Films showed good mucoadhesive properties and guaranteed a prolonged release of viable lactobacilli, which can be directed towards the whole buccal cavity or specific mucosa lesions. In conclusion, the proposed preparative method can be successfully employed for the production of buccal films able to release viable L. brevis CD2 cells that maintain the anti-inflammatory enzymatic activity.

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Section: Scanning Electron Microscopy (Sem)supporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: In Vitro Residence Time and Mucoadhesive Ability Of Buccal Fmentioning

confidence: 99%

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Mucoadhesive Buccal Films for Local Delivery of Lactobacillus brevis

et al. 2020

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“…Indeed, L. cripatus has been previously indicated as one of the most active species against several urogenital pathogens, including GC (Breshears et al, 2015 ; Nunn et al, 2015 ; Parolin et al, 2015 ), suggesting that a vaginal microbiota dominated by this species can prevent different uro-genital and sexually transmitted microorganisms simultaneously, by means of a plethora of mechanisms. By virtue of its antimicrobial properties, attempts have been done to formulate L. crispatus in vaginal inserts for topical treatment of uro-genital infections (Vitali et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Vaginal Lactobacilli Reduce Neisseria gonorrhoeae Viability through Multiple Strategies: An in Vitro Study

Foschi

Salvo

Cevenini

et al. 2017

Front. Cell. Infect. Microbiol.

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The emergence and spread of antimicrobial resistance in Neisseria gonorrhoeae (GC) underline the need of “antibiotic-free” strategies for the control of gonorrhea. The aim of this study was to assess the anti-gonococcal activity of 14 vaginal Lactobacillus strains, belonging to different species (L. crispatus, L. gasseri, L. vaginalis), isolated from healthy pre-menopausal women. In particular, we performed “inhibition” experiments, evaluating the ability of both lactobacilli cells and culture supernatants in reducing GC viability, at two different contact times (7 and 60 min). First, we found that the acidic environment, associated to lactobacilli metabolism, is extremely effective in counteracting GC growth, in a pH- and time-dependent manner. Indeed, a complete abolishment of GC viability by lactobacilli supernatants was observed only for pH values < 4.0, even at short contact times. On the contrary, for higher pH values, no 100%-reduction of GC growth was reached at any contact time. Experiments with organic/inorganic acid solutions confirmed the strict correlation between the pH levels and the anti-gonococcal effect. In this context, the presence of lactate seemed to be crucial for the anti-gonococcal activity, especially for pH values in the range 4.4–5.3, indicating that the presence of H+ ions is necessary but not sufficient to kill gonococci. Moreover, experiments with buffered supernatants led to exclude a direct role in the GC killing by other bioactive molecules produced by lactobacilli. Second, we noticed that lactobacilli cells are able to reduce GC viability and to co-aggregate with gonococci. In this context, we demonstrated that released-surface components with biosurfactant properties, isolated from “highly-aggregating” lactobacilli, could affect GC viability. The antimicrobial potential of biosurfactants isolated from lactobacilli against pathogens has been largely investigated, but this is the first report about a possible use of these molecules in order to counteract GC infectivity. In conclusion, we identified specific Lactobacillus strains, mainly belonging to L. crispatus species, able to counteract GC viability through multiple mechanisms. These L. crispatus strains could represent a new potential probiotic strategy for the prevention of GC infections in women.

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“…The protective role of lactobacilli against urogenital pathogens is exerted through different mechanisms including the production of various antibacterial compounds (lactic acid, hydrogen peroxide, bacteriocins, and biosurfactants), the competitive exclusion for epithelial adhesion and the immunomodulation ( Kaewsrichan et al, 2006 ; Borges et al, 2014 ; Parolin et al, 2015 ; Younes et al, 2018 ). In this context, the use of probiotic lactobacilli for the prevention and treatment of several urinary and vaginal tract infections has been extensively evaluated, with different results depending on the Lactobacillus species, the strain origin, the concentrations used and the outcome considered ( Barrons and Tassone, 2008 ; Bolton et al, 2008 ; Spurbeck and Arvidson, 2011 ; Vitali et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus crispatus BC5 Interferes With Chlamydia trachomatis Infectivity Through Integrin Modulation in Cervical Cells

et al. 2018

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Lactobacilli play a crucial role in maintaining the ecological equilibrium of the vaginal niche, preventing the colonization of exogenous microorganisms. Although many studies have discussed the mechanisms displayed by lactobacilli in counteracting several urogenital pathogens, a few data are available on the interaction between lactobacilli and Chlamydia trachomatis. This study aimed to elucidate the molecular bases of the interaction among vaginal lactobacilli, the sexually transmitted pathogen C. trachomatis and the epithelial cervical cells. We evaluated the in vitro activity of 15 Lactobacillus strains, belonging to different species (i.e., L. crispatus, L. gasseri, L. vaginalis), against C. trachomatis. In particular, we evaluated the capability of lactobacilli cells to interfere with C. trachomatis infection in HeLa cells, by exclusion assays. Lactobacilli significantly reduced C. trachomatis infectivity, being L. crispatus the most active species. Although a dose-dependent effect was noticed, a significant antagonistic activity was maintained even at lower doses. As other Gram-positive bacteria (i.e., Streptococcus agalactiae, Enterococcus faecalis, and Bacillus subtilis) failed to interfere with C. trachomatis infectivity, Lactobacillus activity proved to be specific. The potential mechanism of protection was investigated in Lactobacillus crispatus BC5, chosen as the model strain. The incubation of HeLa cell line with BC5 cells induced important modifications in the epithelial plasma membrane, by altering lipid composition and α5 integrin subunit exposure. When α5 integrin subunits were masked by a specific blocking antibody or ITGA5 gene expression was silenced, Chlamydia infection was significantly reduced. It follows that α5 integrin subunit is crucial for the pathogen infection process, and the anti-Chlamydia activity can be directly linked to membrane properties modifications in cervical cells. The three Gram-positive bacteria used as controls failed to modify the expression of α5β1 integrin. In conclusion, we identified a potential molecular mechanism at the basis of the protection exerted by L. crispatus BC5 against C. trachomatis, getting insights into the role of the cervico-vaginal microbiota for the woman’s health.

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Association of Lactobacillus crispatus with fructo-oligosaccharides and ascorbic acid in hydroxypropyl methylcellulose vaginal insert

Cited by 27 publications

References 40 publications

Mucoadhesive Buccal Films for Local Delivery of Lactobacillus brevis

Mucoadhesive Buccal Films for Local Delivery of Lactobacillus brevis

Vaginal Lactobacilli Reduce Neisseria gonorrhoeae Viability through Multiple Strategies: An in Vitro Study

Lactobacillus crispatus BC5 Interferes With Chlamydia trachomatis Infectivity Through Integrin Modulation in Cervical Cells

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