Association of low-density lipoprotein with particulate connective tissue matrix components enhances cholesterol accumulation in cultured subendothelial cells of human aorta

Effect of Lipoproteins and Platelets on Macrophage Cholesterol Metabolism

“…Components of the extracellular matrix such as arterial proteoglycans can interact with LDL to form an insoluble complex that enhances CE accumulation in macrophages through specific binding sites that are different from LDL or scavenger receptors (Salisbury et at., 1985). Association of LDL with particulate connective tissue matrix components such as elastin and collagenase-resistant debris isolated from human aorta also enhances cholesterol accumulation in cultured subendothelial cells of human aorta (Orekhov et at., 1987).…”

Autoantibodies against modified low density lipoprotein. Nonlipid factor of blood plasma that stimulates foam cell formation.

“…Cellular phospholipids, triglycerides, free cholesterol, and cholesteryl esters were separated by thin-layer chromatography and measured by scanning densitometry as described earlier. 22 Using light and electron microscopy, 25 ' 26 we have demonstrated that an increase of cellular lipids is associated with the appearance of intracellular lipid inclusions.…”

Fibronectin stimulates macrophage uptake of low density lipoprotein-heparin-collagen complexes.

“…1 " 4 Our previous experiments have shown that LDL incorporated into a model extracellular matrix containing heparin, fibronectin (Fn), and denatured collagen (gelatin) was readily phagocytosed by macrophages. 1 However, in contrast to either LDL or chemically modified LDL, in which the degradation of lipoprotein paralleled the uptake, the endocytosis of insoluble LDL matrix complexes rapidly exceeded the macrophage's capacity to degrade LDL.…”