Association of Mannose-Binding Lectin rs1800450 and Tumor Necrotic Factor-α rs1800620 Polymorphism with Helicobacter pylori in Type II Diabetes Mellitus

Abstract

Type II diabetes mellitus (T2DM) is the prevalent type of diabetes, including 90% of the cases world-wide. Helicobacter pylori plays a pathogenic role in the development of T2DM. The host genetic factors have a significant impact on the clinical outcome and anatomical distribution of H. pylori infection and polymorphisms in several genes such as tumor necrotic factor (TNF)-α and mannose-binding lectin (MBL) and are considered to increase the risk for the development of T2DM. In this study, we investigate the prevalence rate of H. pylori infection and its relationship to MBL rs1800450 and TNF-α rs1800620 polymorphism in T2DM. In this case-control study, 174 patients with type II diabetes and 185 healthy controls were studied. Also, demographics, physical, and biochemical parameters were performed in all patients. The DNA extracted from blood specimens was amplified by H. pylori cagA-specific primers. The MBL rs1800450 and TNF-α rs1800620 genotyping were detected by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). The results show that H. pylori cagA positivity was detected in 42.82% of the diabetic patients and in 22.16% of the control group, and H. pylori infection was closely correlated with MBL rs1800450 AA genotype and TNF-α rs1800620 GG genotype when compared with healthy controls. Furthermore, these two genotypes were strongly associated with H. pylori cagA(+) samples when compared with cagA(-) samples. In addition, the presence of H. pylori cagA(+) infection was significantly associated with the elevated serum levels of total cholesterol and low-density lipoprotein cholesterol. In general, it can be concluded that molecular analysis of MBL rs1800450 AA genotype and TNF-α rs1800620 AA genotype is important in the early detection and treatment of T2DM with H. pylori cagA(+) infection.

Gut Microbiota: The Missing Link Between Helicobacter pylori Infection and Metabolic Disorders?

“…The link between H. pylori and Type 2 Diabetes was first analyzed in 1989 by Simon et al, who found that the prevalence of H. pylori infection was higher in patients with Type 2 Diabetes compared to asymptomatic volunteers (84). Thenceforth, most of the studies have corroborated this finding reporting a higher prevalence of H. pylori in patients with Type 2 Diabetes (usually greater than 50%) in diabetic patients than in non-diabetic subjects (85)(86)(87)(88). Recently published meta-analyses this year aimed at elucidating the direction of the relationship between H. pylori and Type 2 Diabetes (81).…”

Mechanisms of the Epithelial–Mesenchymal Transition and Tumor Microenvironment in Helicobacter pylori-Induced Gastric Cancer

“…H. pylori induces other diseases of the alimentary tract, including gastritis, peptic ulcer disease, mucosa-associated lymphoid tissue lymphoma, GERD symptoms, and dyspepsia [41][42][43][44][45][46]. There is an increasing interest in the role of H. pylori in the pathogenesis of ischemic heart disease, diabetes mellitus, and Alzheimer's disease [47][48][49][50].…”

Течение Пептических Гастродуоденальных Язв На фоне Коморбидного Сахарного Диабета

“…Серопозитивность больных СД по Нр составляет около 60 % [19], а носительство инфекции регистрируется на 90 % чаще, чем в общей популяции, что прямо коррелирует с уровнями в крови фактора некроза опухоли α, маннозосвязанного лектина и липопротеинов низкой плотности. С хеликобактерной инфекцией у таких больных тесно связаны генотипы rs1800450 AA и rs1800620 [20]. Выводы 1.…”