Association of Matrix Metalloproteinase-2 (MMP-2) and MMP-9 Promoter Polymorphisms, Their Serum Levels, and Activities with Coronary Artery Calcification (CAC) in an Iranian Population

elahirad, saeed; Komi, Daniel Elieh Ali; Kiani, Amir; Mohammadi‐Noori, Ehsan; Vaisi‐Raygani, Asad; Mozafari, Hadi; Bahrehmand, Fariborz; Mh, Saidi; Toupchi-Khosroshahi, Vahid; Salehi, Nahid

doi:10.1007/s12012-021-09707-5

Cited by 12 publications

(8 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, these variables were not examined in this study. Similarly, in an Iranian study, serum MMP-2 and MMP-9 were elevated in patients with recent symptoms suggestive of CAD and proven elevated coronary artery calcium (CAC) scores compared to healthy controls [29]. On the other hand, sub-analysis of our CAD cases (self-reported IHD and previous angiography or angioplasty) revealed no association with MMP-2 or MMP-9 levels.…”

Section: Association Of Biomarkers With Cvdmentioning

confidence: 58%

“…In Model 1.2, age, gender, and ethnicity were added to the model if these variables did not already enter the model. In Model 1.3, the models differed slightly for each biomarker because additional variables were added based on previously published literature as potential factors of the individual biomarker [9,[24][25][26][27][28][29][30][31]. For RANTES, Model 1.3 was further adjusted for history of gout, metabolic syndrome, body mass index (BMI), serum creatinine, and hs-CRP.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Factors Associated with RANTES, EMMPIRIN, MMP2 and MMP9, and the Association of These Biomarkers with Cardiovascular Disease in a Multi-Ethnic Population

Wang

Tan

Lai

et al. 2022

JCM

View full text Add to dashboard Cite

Background: The growing cardiovascular disease (CVD) epidemic calls for further research to identify novel biomarkers for earlier detection and as potential therapeutic targets. Biomarkers Regulated on Activation, Normal T Cell Expressed and Secreted (RANTES), extracellular matrix metalloproteinase inducer (EMMPRIN), and matrix metalloproteinases (MMP-2, and MMP-9) are linked to proatherogenic and proinflammatory pathways of CVD development, the majority of which are coronary artery disease (CAD) and stroke. We evaluated potential factors affecting these four biomarkers and established their association with CVD. Methods: This is a cross-sectional analysis using a nested case-control design involving 580 participants aged 21–75 years from the prospective multi-ethnic cohort study. A total of 290 CVD cases and 290 age-and sex-matched controls were identified. All participants underwent interviews, health screenings, and provided blood samples, including biomarkers RANTES, EMMPRIN, and MMPs. CVD was defined based on previous medical history. Results: The average age of the participants was 55.7(SD = 10.3) years of age, and 34.6% were female. Arrhythmia history and low-density lipoprotein (LDL) levels were significant factors of logEMMPRIN (β = −0.124 [−0.245, −0.003] and β = 0.111 [0.0, 0.191], respectively). Only female sex (β = 0.189 [0.078, 0.300]) for logRANTES and age (β = 0.033 [0.010, 0.055]) for logMMP-2 and logMMP-9 were significant. The Indian ethnicity (β = 0.192 [0.048, 0.335]) and highly sensitive C-reactive protein (hs-CRP) levels (β = 0.063 [0.011, 0.116]) were statistically significant for logMMP-9. No association was detected between biomarkers and CVD. Conclusions: In this multi-ethnic study cohort, RANTES was associated with sex, EMMPRIN was associated with a history of arrhythmia and LDL levels, MMP-2 with age, and MMP-9 with ethnicity and hs-CRP levels. The biomarker serum levels were not associated with CVD.

show abstract

Section: Association Of Biomarkers With Cvdmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Factors Associated with RANTES, EMMPIRIN, MMP2 and MMP9, and the Association of These Biomarkers with Cardiovascular Disease in a Multi-Ethnic Population

Wang

Tan

Lai

et al. 2022

JCM

View full text Add to dashboard Cite

show abstract

“…Tryptase is involved in angiogenesis, degradation of fibrous and amorphous components of the extracellular matrix of connective tissue, and release of growth factors, which can be the results of tryptase-mediated activation of MMPs, mainly MMP-2 and MMP-9 [18]. These endopeptidases play a crucial role in the degradation of structural elements of ECM such as collagen [43,44].…”

Section: Discussionmentioning

confidence: 99%

Space-Flight- and Microgravity-Dependent Alteration of Mast Cell Population and Protease Expression in Digestive Organs of Mongolian Gerbils

Atiakshin,

Kostin,

Shishkina

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Mast cell (MC)-specific proteases are of particular interest for space biology and medicine due to their biological activity in regulating targets of a specific tissue microenvironment. MC tryptase and chymase obtain the ability to remodel connective tissue through direct and indirect mechanisms. Yet, MC-specific protease expression under space flight conditions has not been adequately investigated. Using immunohistochemical stainings, we analyzed in this study the protease profile of the jejunal, gastric, and hepatic MC populations in three groups of Mongolian gerbils—vivarium control, synchronous experiment, and 12-day orbital flight on the Foton-M3 spacecraft—and in two groups—vivarium control and anti-orthostatic suspension—included in the experiment simulating effects of weightlessness in the ground-based conditions. After a space flight, there was a decreased number of MCs in the studied organs combined with an increased proportion of chymase-positive MCs and MCs with a simultaneous content of tryptase and chymase; the secretion of specific proteases into the extracellular matrix increased. These changes in the expression of proteases were observed both in the mucosal and connective tissue MC subpopulations of the stomach and jejunum. Notably, the relative content of tryptase-positive MCs in the studied organs of the digestive system decreased. Space flight conditions simulated in the synchronous experiment caused no similar significant changes in the protease profile of MC populations. The space flight conditions resulted in an increased chymase expression combined with a decreased total number of protease-positive MCs, apparently due to participating in the processes of extracellular matrix remodeling and regulating the state of the cardiovascular system.

show abstract

“…According to CLSI guidelines 41 when the correlation coefficient is greater than 0.975, simple linear regressions could be used to further explore constant and proportional errors; otherwise, more rigorous analyses such as Deming or Passing-Bablok regressions are needed 42 . Numerous studies quantified plasma MMP-9 in different pathologies, by gel zymography along with ELISA [43][44][45][46] . Heo et al focused on method comparison of gel zymography and ELISA to ensure the reliability of the measurements and their results were based only on the coefficient of correlation 39 .…”

Section: Discussionmentioning

confidence: 99%

Clinical significance of matrix metalloproteinase-9 in Fragile X Syndrome

Laroui

Galarneau

Abolghasemi

et al. 2022

Sci Rep

View full text Add to dashboard Cite

High plasma matrix metalloproteases-9 (MMP-9) levels have been reported in Fragile X Syndrome in a limited number of animal and human studies. Since the results obtained are method-dependent and not directly comparable, the clinical utility of MMP-9 measurement in FXS remains unclear. This study aimed to compare quantitative gel zymography and ELISA and to determine which method better discriminates abnormal MMP-9 levels of individuals with FXS from healthy controls and correlates with the clinical profile. The active and total forms of MMP-9 were quantified respectively, by gel zymography and ELISA in a cohort of FXS (n = 23) and healthy controls (n = 20). The clinical profile was assessed for the FXS group using the Aberrant Behavior Checklist FXS adapted version (ABC-CFX), Adaptive Behavior Assessment System (ABAS), Social Communication Questionnaire (SCQ), and Anxiety Depression and Mood Scale questionnaires. Method comparison showed a disagreement between gel zymography and ELISA with a constant error of − 0.18 [95% CI: − 0.35 to − 0.02] and a proportional error of 2.31 [95% CI: 1.53 to 3.24]. Plasma level of MMP-9 active form was significantly higher in FXS (n = 12) as compared to their age-sex and BMI matched controls (n = 12) (p = 0.039) and correlated with ABC-CFX (rs = 0.60; p = 0.039) and ADAMS (rs = 0.57; p = 0.043) scores. As compared to the plasma total form, the plasma MMP-9 active form better enables the discrimination of individuals with FXS from controls and correlates with the clinical profile. Our results highlight the importance of choosing the appropriate method to quantify plasma MMP-9 in future FXS clinical studies.

show abstract

Association of Matrix Metalloproteinase-2 (MMP-2) and MMP-9 Promoter Polymorphisms, Their Serum Levels, and Activities with Coronary Artery Calcification (CAC) in an Iranian Population

Cited by 12 publications

References 46 publications

Factors Associated with RANTES, EMMPIRIN, MMP2 and MMP9, and the Association of These Biomarkers with Cardiovascular Disease in a Multi-Ethnic Population

Factors Associated with RANTES, EMMPIRIN, MMP2 and MMP9, and the Association of These Biomarkers with Cardiovascular Disease in a Multi-Ethnic Population

Space-Flight- and Microgravity-Dependent Alteration of Mast Cell Population and Protease Expression in Digestive Organs of Mongolian Gerbils

Clinical significance of matrix metalloproteinase-9 in Fragile X Syndrome

Contact Info

Product

Resources

About