Association of Microsatellites with Growth and Immunocompetence Traits in Crossbred Layer Chicken

Abstract

The present investigation was carried out on six crossbred chicken populations to estimate variability of microsatellites and their association with growth and other traits. Five microsatellite markers located on chromosome , , and were screened and association study was performed following general linear model technique. All the microsatellites were polymorphic showing three to six alleles. The polymorphic information content (PIC) of the markers was more than . . Genotype and allelic frequency was estimated showing a large variability from microsatellite to microsatellite. The genotypes of microsatellite were found to be significantly ( . ) associated with body weight at day old, , , , and week of age. A significant association between microsatellite and body weight at , and week of age was estimated at . in di erent crossbred chicken populations. genotypes were observed to be significantly associated with body weight at weeks of age. and microsatellites were found to be significantly correlated with age at sexual maturity whereas humoral immune response to sheep RBC were observed to be non-significantly associated with the microsatellites.

Microsatellite Variability and Its Relationship with Growth, Egg Production, and Immunocompetence Traits in Chickens

“…This indicated that the MCW0041 microsatellite marker was probably in linkage disequilibrium with the QTLs of body weight at 28 and 40 weeks of age. Significant associations of some microsatellites with body weight at various ages were observed by earlier researchers (Van Kaam et al 1998, 1999Jennen et al 2006;Chatterjee et al 2008b), but no significant association was observed between any microsatellite and body weight at other ages, age at sexual maturity, or sheep RBC titer. Zhou et al (2003) studied genetic markers associated with antibody response kinetics in adult chickens and concluded that regions on chromosomes 3, 5, 6, and Z contain QTLs that affect antibody kinetics.…”

“…Zhou et al (2008) reported 6.41 alleles per locus in Chinese Silkies for a different set of microsatellite markers. Chatterjee et al (2008b) analyzed five sets of microsatellites and observed that all the microsatellites were polymorphic, with three to six alleles. The genotypic proportions were distributed from very low to very high.…”

Genetic variation of the major histocompatibilty complex-B haplotypes in Nigerian local chicken populations

“…Since microsatellite markers are regarded as informative when their PIC value is greater than or equal to 0.5 (Botstein et al, 1980;Chatterjee et al, 2008), the within and across populations PIC values which are all above 0.9 showed that the LEI0258 marker is highly informative for revealing genetic differentiation within and among the Nigerian local chicken populations. The N m value of 10.620 derived in these populations revealed a significant level of gene flow among the Nigerian chicken populations; similar result was observed when other microsatellite markers from nonfunctional DNA regions were used to study gene flow among Nigerian chicken populations (Olowofeso et al 2016).…”

Polymorphism at exon 1 of pit-1 gene and its association with immunocompetence traits in layer chicken

“…Two immune competence traits, namely response to PHAP (Measure of cell mediated immune response) and SRBC titre (Measure of humoral immune response) in all the birds were measured following standard methods (Chatterjee et al 2008). …”

“…The cell mediated immunity can be assessed by response to phyto haem agglutinin-P (PHAP) in poultry. Both the measures can point out the overall general immune competence of the birds (Chatterjee et al 2008). In this study, we explored the polymorphism of partial promoter and exon 1 of pit-1 gene and its effect on immunocompetence traits in White Leghorn chicken.…”