Association of MiRNA-146a, MiRNA-499, IRAK1 and PADI4 Polymorphisms with Rheumatoid Arthritis in Egyptian Population

Shaker, Olfat G.; El-Boghdady, Noha A.; Sayed, Abeer El-Dessouki El

doi:10.1159/000489592

Cited by 39 publications

(20 citation statements)

References 52 publications

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“…The thermocycling conditions were as follows: 45 PCR cycles at 92˚C for 15 sec; denaturation at 95˚C for 10 min; and annealing and extension at 60˚C for 90 sec. Real-time PCR was performed using a Rotor-gene Q Real-Time PCR system (Qiagen, Inc.) (27).…”

Section: Methodsmentioning

confidence: 99%

Relationship between miR‑155 and miR‑146a polymorphisms and susceptibility to multiple sclerosis in an Egyptian cohort

et al. 2020

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Multiple sclerosis (MS) is an autoimmune disorder of the central nervous system. It was previously demonstrated that miR-155 and miR-146a served a vital role in the pathophysiology of MS, and single nucleotide polymorphisms in miR-155 and miR-146a were found to be associated with the susceptibility to different autoimmune diseases, such as systemic lupus erythematosus, rheumatoid arthritis and type I diabetes. The aim of the present study was to analyze the association between susceptibility to MS and two genetic polymorphisms (miR-155 rs767649 A>T and miR-146a rs57095329 A>G) in a cohort of Egyptian patients. The presence of the two polymorphisms were analyzed in 114 patients with MS and 152 healthy controls using quantitative PCR. The present study demonstrated for the first time that: The TT genotype and T allele in miR-155 (rs767649 A>T) polymorphism were associated with an increased risk of MS; the miR-146a (rs57095329 A>G) mutated G allele conferred protection against the development of MS in all genetic models; miR-155 rs767649 A>T was a risk associated polymorphism of MS in females, but not in males; and miR-155 rs767649 AT/TT and miR-146a rs57095329 GG genotypes showed significantly higher distributions among patients with higher Expanded Disability Status Scale scores and secondary progressive MS subgroups. Therefore, miR-155 rs767649 polymorphism may confer susceptibility to MS, whereas miR-146a rs57095329 may be protective against MS in an Egyptian cohort.

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Section: Methodsmentioning

confidence: 99%

Relationship between miR‑155 and miR‑146a polymorphisms and susceptibility to multiple sclerosis in an Egyptian cohort

et al. 2020

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“…Rheumatoid arthritis (RA) represents a type of autoimmune joint inflammatory disorder and has been reported to affect approximately 6% of people over 65 years old, while showing predominance in women more so than men [1]. The chief characteristics of RA include hyperplasia synovitis and inflammatory immune cell infiltration [2].…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA PVT1 knockdown suppresses fibroblast-like synoviocyte inflammation and induces apoptosis in rheumatoid arthritis through demethylation of sirt6

Zhang

Liú

et al. 2019

J Biol Eng

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Background As a type of chronic autoimmune joint disease, rheumatoid arthritis (RA) is a disorder, characterized by a variety of physical symptoms as well as RA fibroblast-like synoviocyte (RA-FLS) proliferation. More recently, long non-coding RNAs (lncRNAs) have been implicated in the progression of various diseases including the progression of RA. Hence, the aim of the current study was to investigate the role by which the lncRNA, plasmacytoma variant translocation 1 (PVT1), influences RA-FLSs and its ability to modulate the methylation of sirtuin 6 (sirt6) . Methods RA rat models were initially established to determine the expression of PVT1 and sirt6 in synovial tissues and RA-FLSs. Elevation or depletion of PVT1 or sirt6 was achieved by means of transformation with plasmids in order to investigate their effects on RA-FLS proliferation, inflammation and apoptosis. The localization of PVT1 and its binding ability to the sirt6 promoter region were also explored in an attempt to elucidate the correlation between PVT1 and sirt6 methylation. Results High expression of PVT1 and low expression of sirt6 were detected in the synovial tissues and RA-FLSs of the rat models. RA-FLSs treated with sh-PVT1 or oe-sirt6 exhibited suppressed cell proliferation, inflammation and induced apoptosis. PVT1 was predominately localized in the nucleus while evidence was obtained indicating that it could bind to the sirt6 promoter to induce sirt6 methylation, thus inhibiting sirt6 transcription. PVT1 knockdown was observed to restore sirt6 expression through decreasing sirt6 methylation, thereby alleviating RA. Conclusion The key findings of the study provide evidence suggesting that, PVT1 knockdown is able to restrain RA progression by inhibiting sirt6 methylation to restore its expression.

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“…They play an important role in regulating transcription and translation [3–6]. Some studies indicated that they also participate in cell proliferation, differentiation, and apoptosis [7, 8]. And, miRNAs can cause the degradation or translation repression of their target miRNA by binding to the 3′-untranslated region of specific mRNAs (messenger RNAs) [9–12].…”

Section: Introductionmentioning

confidence: 99%

Association of rs2910164 Polymorphism in miRNA-146 and rs3746444 Polymorphism in miRNA-499 with Inflammatory Arthritis: A Meta-Analysis

Wang

Pan

2019

BioMed Research International

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Objectives. The purpose of this study was to explore the association of miRNA-146 and miRNA-499 polymorphisms with inflammatory arthritis. Methods. A systematic search of studies on the association of miRNA-146 and miRNA-499 polymorphisms with inflammatory arthritis susceptibility was conducted in PubMed, Web of science, Elsevier ScienceDirect, and Cochrane Library. Eventually, 18 published studies were included. The strength of association between miRNA-146/499 polymorphisms and inflammatory arthritis susceptibility was assessed by odds ratios (ORs) with its 95% confidence intervals (CIs). Results. A total of 18 case-control studies, consisting of 3385 inflammatory arthritis patients and 4584 controls, were included in the meta-analysis. This meta-analysis showed significant association between miRNA-499 rs3746444 polymorphism and inflammatory arthritis susceptibility in overall population (C vs T, OR: 1.422, 95% CI= 1.159-1.745, P=0.001). Similar results were found in subgroup analysis by region. But we did not find association between miRNA-146 rs2910164 polymorphism and inflammatory arthritis susceptibility in overall population (C vs T, OR: 1.061, 95% CI= 0.933-1.207, P=0.365). Conclusions. The present study indicates that miRNA-499 rs3746444 polymorphism is associated with inflammatory arthritis susceptibility. However, there is lack of association between miRNA-146 rs2910164 polymorphism and inflammatory arthritis susceptibility. But, we also find miRNA-146 rs2910164 and miRNA-499 rs3746444 polymorphism are associated with inflammatory arthritis in Middle East. Therefore, more large-scale studies are warranted to replicate our findings.

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Association of MiRNA-146a, MiRNA-499, IRAK1 and PADI4 Polymorphisms with Rheumatoid Arthritis in Egyptian Population

Cited by 39 publications

References 52 publications

Relationship between miR‑155 and miR‑146a polymorphisms and susceptibility to multiple sclerosis in an Egyptian cohort

Relationship between miR‑155 and miR‑146a polymorphisms and susceptibility to multiple sclerosis in an Egyptian cohort

Long non-coding RNA PVT1 knockdown suppresses fibroblast-like synoviocyte inflammation and induces apoptosis in rheumatoid arthritis through demethylation of sirt6

Association of rs2910164 Polymorphism in miRNA-146 and rs3746444 Polymorphism in miRNA-499 with Inflammatory Arthritis: A Meta-Analysis

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