Association of MMP9 Polymorphism With Myocardial Infarction

Abstract

Myocardial Infarction (MI) is the most common type of coronary heart disease characterized by pain, nausea, sweating, shortness of breath and abnormal heartbeat. Current study was conducted to identify the susceptibility of the MMP-9 gene among families affected with myocardial infarction in the Pakistani population. A family clustering study based on 5 families having MI patients was conducted. Blood samples from MI patients and their family members were collected for further genetic analysis. Patients were with a mean BMI of ±30.2 kg/m2 which indicates that obese were at high risk of disease development. The mean age for diagnosis of disease was ±50 years, in which all patients had a positive family history. Most patients were diabetic with hypertension, most of them were smokers and their exposure to air pollution was higher. Genomic DNA was isolated from the blood through manual extraction. Primers were optimized and genotyping was done by polymerase chain reaction (PCR) which was followed by DNA sequencing and restriction fragment length polymorphism (RFLP). As a result of polymorphism, A into G and C into T conversions were identified on rs17576 and rs3918242 polymorphic sites on the MMP-9 gene respectively. In conclusion smoking, hypertension, diabetes, exposure to air pollution and polymorphism of rs17576 and rs3918242 were significantly associated with the onset of MI in the Pakistani population and males were at higher risk. Further studies should be conducted on large scale to evaluate the association of MMP-9 polymorphism with MI.

Association of the Polymorphism rs3918242 of the Matrix Metalloproteinase-9 Gene with Coronary Artery Disease in a Younger Population

“…Regarding the allelic frequencies, the statistical analysis showed that the T-allele is a risk allele that contributes to a significant association (p<0.05) of the rs3918242 polymorphism with myocardial infarction (higher allelic frequency in patients versus controls). What can be concluded from this study is that both mentioned polymorphisms of the MMP9 gene have a significant role in the development of myocardial infarction, whereas the TT and CT genotypes of rs3918242 indicate that the person is a carrier and may later develop myocardial infarction [30].…”