Association of MTHFR gene polymorphisms with migraine in North Indian population

Abstract

Polymorphisms in MTHFR gene are mostly associated with increased levels of homocysteine in the absence of dietary folate and are a risk factor for complex neurovascular diseases like migraine. The aim of present case-control study was to determine the association between MTHFR gene polymorphisms (C667T; rs 1801133, A1298C; rs 1801131) with migraine susceptibility. In total, 100 patients with migraine (23with MA and 77 with MO) and age-sex matched 100 healthy controls were included in this study from OPD of ESIC Medical College & Hospital, Faridabad. Genotyping was done by PCR-RFLP method. Genotypic and allelic frequencies were compared by SPSS 24 version. Genotypic results indicated a non-significant increase in frequencies of CT and TT in C667T SNP in migraine patients with control (52 and 10% vs. 42 and 7%: p > 0.05), but CC genotype in A1298C was found to be a risk factor in migraine patients than controls (30 vs. 17% respectively: p < 0.05). On comparing migraine subclasses, migraine with aura (MA) and without aura (MO) with control groups, the present study suggests that in MTHFR polymorphisms, the prevalence of 677CT genotype and T allele in C667T SNP influences susceptibility to MA (p < 0.05) but not to MO. Meanwhile, CC genotype in A1298C SNP could be a risk factor for migraine patients without aura (p < 0.05).

Methylenetetrahydrofolate reductase polymorphism (MTHFR C677T) and headache in children: a retrospective study from a tertiary level outpatient service

“…In our study we also showed a significant, though smaller, association between the T/T genotype and MOA (up to 28.8% have T/T polymorphism), as already reported in other studies in non-Caucasian pediatric and adult populations [4, 6–9, 12, 13]. …”

Effects of MTHFR C677T and A1298C Polymorphisms on Migraine Susceptibility: A Meta‐Analysis of 26 Studies

“…Five studies (4 in Caucasians and 1 in Indians) including 1368 cases and 1411 controls focused on MTHFR A1298C polymorphism and migraine . Genotype distributions in control groups of all 5 studies were consistent with HWE.…”

“…Main characteristics of these 26 studies were summarized in Table . All 26 studies focused on MTHFR C677T polymorphism, and 5 studies also investigated A1298C polymorphism . The numbers of studies recruiting Caucasians, Asians, Indians, and Pakistanis were 20, 3, 2, and 1, respectively.…”

“…Studies.-After retrieving the electronic databases, we initially identified 653 articles. Six hundred twentysix articles were excluded for various reasons and 26 studies (23 case-control studies and 3 cross-sectional studies) with 10,228 migraineurs and 28,608 controls were deemed eligible according to the inclusion and exclusion criteria [10][11][12][13][14][18][19][20][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] (Fig. 1 shows the selection process).…”

“…All 26 studies focused on MTHFR C677T polymorphism, and 5 studies also investigated A1298C polymorphism. 19,20,30,31,45 The numbers of studies recruiting Caucasians, Asians, Indians, and Pakistanis were 20, 3, 2, and 1, respectively. Two studies only included MA suffers.…”