Association of NFkB1 Gene Polymorphism with Inflammatory Markers in Patients of Type 2 Diabetes Mellitus with or without Renal Involvement in Eastern India

Abstract

Aims: To evaluate the association of Nuclear factor kappa B1(NFkB1) gene polymorphism with inflammatory markers Urinary Monocyte Chemoattractant Protein 1 (UMCP1) and Tumor Necrosis Factor alfa (TNF alfa) in Patients of diabetes mellitus with or without renal involvement in Eastern India. Material and Methods: Consecutive Patients of Type 2 Diabetes Mellitus (DM) with or without microalbuminuria attending SCB MEDICAL COLLEGE and HOSPITAL Medical OPDs in between September 2018 to September 2019 were recruited in this study. Patients were subjected to blood and urine investigations. DNA extraction and Restriction fragment Length Polymorphism (RFLP) was done in Department of Biochemistry. Controls were unrelated healthy attendants with no history of Diabetes Mellitus, HTN, Chronic Kidney Disease (CKD). Results: Mean Systolic BP, Fasting Blood Glucose, Post Prandial Blood Glucose, HBA1c, Total Cholesterol were significantly higher in diabetes mellitus and diabetic nephropathy groups than control group. Estimated Glomerular Filtration Rate was significantly lower in diabetic nephropathy (p value < 0.001). UMCP1, Urinary Albumin Creatinine Ratio, TNF alfa were higher in diabetes mellitus and nephropathy with p value (<0.001, 0.006 < 0.001) respectively. In between DM and Diabetic Nephropathy groups nfkb1 gene expression, umcp1 and tnf alfa levels were significantly increased in Diabetic nephropathy with p value 0.019, <0.01, 0.001 respectively. Insertion/insertion NFkB1 gene polymorphisms were more in diabetic nephropathy group and were positively correlated with inflammatory markers UMCP1 (r = 0.517, p < 0.01) and TNF alfa (r = 0.172, p = 0.19). Conclusion: insertion/insertion NFkB1 gene polymorphism increases the risk of nephro

Diabetic nephropathy patients show hyper-responsiveness to N6-carboxymethyllysine

“…Several studies have reported elevated levels of serum TNF in DN patients and T2DM. In addition, the relationship was further confirmed in a meta-analysis ( 23 ). However, there is limited information on the mechanisms underlying the elevated inflammatory markers in DN and T2DM.…”

Nutrigenomics in Regulating the Expression of Genes Related to Type 2 Diabetes Mellitus

“…Hyperglycemia promotes the formation of advanced glycation end-products (AGEs) that induce inflammation and oxidative stress, so polymorphisms in the AGER gene have been associated with the risk of type 2 diabetes ( Kang et al, 2012 ; Lin et al, 2012 ). In addition, the inhibition of the NFKB1 and NFKB2 genes shows a decrease in the inflammatory process, consequently improving hypertension and suppressing vasoconstriction induced by diabetes ( Gautam et al, 2017 ; Behera et al, 2020 ). In patients with T2DM, we can also observe an increased expression of the FTO gene, which may be involved in oxidative metabolism, lipogenesis, and oxidative stress ( Bravard et al, 2011 ).…”