Association of nitric oxide synthase 3 gene polymorphism with the risk of type 2 diabetes

Abstract

Abstract. Type-2 diabetes (T2D) is a multifactorial (environmental and genetic factors) and global epidemic disease with an estimated high prevalence worldwide. Studies have indicated that nitric oxide synthase 3 (NOS3) has several important roles in the pathogenesis of T2D. The present study aims to investigate the association between NOS3 rs1800779(A/G) and T2D in an Iranian sample population. A case-control study was conducted on 250 T2D patients and 250 healthy control subjects (HCs). Genotyping of the rs1800779(A/G) variant was conducted using a Tetra-Amplification Refractory Mutation System polymerase chain reaction. The frequencies of genotypes AA, AG and GG polymorphisms were 56.8, 39.2 and 4% in the T2D group, and 42.8, 56 and 1.2% in the HCs group, respectively. The frequency of the minor (G) allele was 23.6% in the T2D group and 29.2% in the HCs group. The genotype frequencies of the rs1800779(A/G) variant demonstrated statistically significant differences between T2D and controls in a codominant model (AG vs. AA, OR=0.527, 95% CI=0.368-0.756, P<0.001) and dominant model (AG+GG vs. AA, OR=0.569, P=0.002). There was no significant association between clinical and demographic characteristics and the NOS3 rs1800779(A/G) polymorphism in dominant status (P>0.05). The dominant model and AG genotype of NOS3 rs1800779(A/G) polymorphism may had a protective effect on T2D of Iranian population.

Evaluating anthracycline cardiotoxicity associated single nucleotide polymorphisms in a paediatric cohort with early onset cardiomyopathy

“…These frequencies range from 4 to 82%. Several of these genes have been previously identified in association with other diseases [35,36]. Of the variants previously identified, missense variants are reported in eight genes.…”

Association of arsenic-induced cardiovascular disease susceptibility with genetic polymorphisms

“…Garme et al . (2017) reported that the AA genotype of rs3918188 SNP of NOS3 gene, may cause an individual to be more susceptible to develop T2DM 53 . Zhao et al .…”

Lack of association between the eNOS rs1800779 (A/G) polymorphism and the myocardial infarction incidence among the Iraqi Kurdish population

“…For genotyping the eNOS rs1800779 polymorphism, the ARMS-PCR test was used. The primers were taken from previously published literature 23 ( Table 3 ). First, 2 μL of genomic-extracted DNA, 10 μL of Taq DNA Polymerase 2× Master Mix RED (Ampliqon, Danish), 1 μL of each forward (inner and outer) and reverse (inner and outer) primer, and 4 μL of nuclease-free water were used for a total of 20 μL.…”

“…The eNOS rs1800779 polymorphism has been considered to be related to migraine disorder 22 and type 2 diabetes mellitus (T2DM). 23 …”