Association of non-synonymous SNPs of <i>OPN</i> gene with litter size traits in pigs

Abstract

Abstract. Osteopontin (OPN) gene is a secreted phosphoprotein which appears to play a key function in the conceptus implantation, placentation and maintenance of pregnancy in pigs. The objectives of this study were to verify the non-synonymous single nucleotide polymorphisms (SNPs) and their association with litter size traits in commercial Thai Large White pigs. A total of 320 Thai Large White sows were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Three SNPs at c.425G>A, c.573T>C and c.881C>T revealed amino acid exchange rates of p.110Ala>Thr, p.159Val>Ala and p.262Pro>Ser, respectively, and were then segregated. These three SNPs were significantly associated with total number born (TNB) and number born alive (NBA) traits. No polymorphisms of the two SNP markers (c.278A>G and c.452T>G) were observed in this study. Moreover, the SNPs at c.425G>A and c.573T>C were found to be in strong linkage disequilibrium. The association of OPN with litter size emphasizes the importance of porcine OPN as a candidate gene for reproductive traits in pig breeding.

Association of single-nucleotide polymorphisms in <i>NAT9 </i>and <i>MAP3K3</i> genes with litter size traits in Berkshire pigs

“…The present study aimed to identify molecular markers that can be applied for breeding in order to improve litter size in Berkshire pigs. Although Berkshire pigs are the most popular with consumers owing to their excellent meat quality, this breed has a smaller litter size than that of the other breeds (Suzuki et al, 2003;Lee et al, 2015). Genetic markers have been developed for improving the litter size traits of Berkshire pigs in a previous study, and the SNPs in IGFBP2 and IGFBP3 are linked to litter size traits in Berkshire pigs (An et al, 2017).…”

“…Berkshire pigs have excellent meat quality in terms of marbling, juiciness, tenderness, and flavor, and meat of this breed is the most popular in South Korea and Japan (Lee et al, 2015). However, Berkshire pigs have smaller litter sizes than the other breeds have (McMullen, 2006).…”

Application of Reproduction Biotechnology and Molecular Genetic to Improve Productivity of Local Pigs

“…Rothschild et al (2011) mendapatkan QTL pada berbagai bangsa babi untuk seleksi pada umur pertama pubertas (delapan bulan) berada pada kromosom nomor 1, 4, 6, 7, 8, 10, 12, 13 dan 15, litter size pada kromosom 1, 6, 7, 8, 10, 12, 14, 15 dan 17 dan litter size hidup pada kromosom nomor 6, 7, 11,13, 16, 17 dan 18. Beberapa gen yang telah ditemukan SNPs-nya secara signifikan berkaitan dengan litter size yaitu gen estrogen receptor (ESR); prolactine receptor (PRLR), retinol binding protein 4 (RBP4) (Rothschild 2000), dan gen osteoponting (OPN) (Kumchoo & Mekchay 2015). Disamping itu, Kumchoo & Mekchay (2015) juga mendapatkan QTL kesuburan pejantan yaitu pada kromosom nomor 3, 4, 6, 9, 15, 17 dan 18.…”

“…Beberapa gen yang telah ditemukan SNPs-nya secara signifikan berkaitan dengan litter size yaitu gen estrogen receptor (ESR); prolactine receptor (PRLR), retinol binding protein 4 (RBP4) (Rothschild 2000), dan gen osteoponting (OPN) (Kumchoo & Mekchay 2015). Disamping itu, Kumchoo & Mekchay (2015) juga mendapatkan QTL kesuburan pejantan yaitu pada kromosom nomor 3, 4, 6, 9, 15, 17 dan 18. (Verardo et al 2014) menerapkan QTL dan mendapatkan 18 SNPs berasosiasi signifikan dengan litter size dan 57 SNPs berasosiasi signifikan dengan jumlah puting.…”

Detection of one large insertion/deletion (indel) and two novel SNPs within the <i>SPEF2</i> gene and their associations with male piglet reproduction traits

“…Then, each individual was assessed to detect whether there was a variation. PCR reactions were performed with touch-down PCR in a 25 µL volume containing 50 ng of genomic DNA, 0.4 µM of two of each primer, 1 × buffer (including 1.5 mM of MgCl 2 , 200 µM of dNTPs and 0.625 units of Taq DNA polymerase; MBI, Vilnius, Lithuania) (Kumchoo et al, 2015). The touch-down PCR protocol was as follows: initial denaturation for 5 min at 95 • C, followed by 18 cycles of denaturation for 30 s at 94 • C; annealing for 30 s at 68 • C (with a decrease of 1 • C per cycle); extension for 1-3 min at 72 • C; another 23 cycles of 30 s at 94 • C, 30 s at 50 • C, and 2 min at 72 • C; and a final extension for 10min at 72 • C, with subsequent cooling to 4 • C. The PCR products were analyzed by means of agarose gel electrophoresis.…”