Association of novel polymorphisms in the bovine myocyte enhancer factor 2D (MEF2D) gene with carcass traits of Polish Holstein-Friesian cattle

Juszczuk-Kubiak, Edyta; Wicińska, K.; Oprządek, J.

doi:10.17221/6824-cjas

Cited by 4 publications

(1 citation statement)

References 40 publications

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“…The MEF2D gene is significantly correlated with the density and diameter of three muscle fibers in goats (Cheng, 2012). The MEF2D gene had SNPs that were significantly related to the lean meat rate of cattle (Juszczuk-Kubiak et al, 2013). A microsatellite polymorphism has been identified in exon 9 of the MEF2D gene with CAG as a repeat unit, which had a significant correlation with the breast muscle weight eRBCA-2020-1422 eRBCA-2020-1422 of Jianchang duck (Hu, 2014).…”

Section: Introductionmentioning

confidence: 99%

Microsatellite Polymorphism and Prokaryotic Expression of Mef2d in Xingyi Duck

Xincai

Hui

Zhonghai³

et al. 2021

Braz. J. Poult. Sci.

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Myocyte enhancer factor 2D (MEF2D) are members of the myocyte enhancer factor 2 (MEF2), a supergene family and are thought to be related to the development and regeneration of skeletal muscle. We selected a microsatellite locus located in the MEF2D gene to study the slaughter characteristics of Xingyi duck and discuss whether the locus could be used as a molecular genetic marker associated with the slaughter characteristics. To further study the function of this gene, we cloned the coding region of the MEF2D gene and expressed it in the prokaryotic expression system. We amplified exon 9 of MEF2D gene by PCR and analyzed after sequencing. The entire CDS region was amplified by RT-PCR. The prokaryotic expression vector was constructed by double enzyme digestion. Results showed that there was a significant correlation between the microsatellite polymorphism of exon 9 of the MEF2D gene and the eviscerated weight rate of Xingyi duck (p<0.05). The eviscerated weight rate of the aa (40/40) genotype was significantly higher than that of the ab (40/49) genotype. The CDS region of the MEF2D gene was cloned with a length of 1557 bp. The prokaryotic expression vector pET32a(+)-MEF2D was constructed. The results provide a foundation for future studies examining the function of the MEF2D.

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Section: Introductionmentioning

confidence: 99%

Microsatellite Polymorphism and Prokaryotic Expression of Mef2d in Xingyi Duck

Xincai

Hui

Zhonghai³

et al. 2021

Braz. J. Poult. Sci.

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Whole genome resequencing reveals the genetic contribution of Kazakh and Swiss Brown cattle to a population of Xinjiang Brown cattle

Chen

Liu

Zhang

et al. 2022

Gene

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Discovery, Characterization, and Functional Study of a NovelMEF2DCAG Repeat in Duck (Anas platyrhynchos)

Wang

Liu

et al. 2016

DNA and Cell Biology

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Myocyte enhancer transcription factor 2D (MEF2D) is an important transcription factor for promoting the growth and development of muscle. CAG repeats have been found in the coding sequence (CDS) of avian MEF2D; however, their functions remain unknown and require further investigation. Here, we examined the characteristics and functional role of MEF2D CAG repeat in duck. The full-length CDS of duck MEF2D was cloned for the first time, and a novel CAG repeat was identified and located in exon 9. Sequence analysis indicated that the protein domains of duck MEF2D are highly conserved relative to other vertebrates, whereas MEF2D CAG repeats with variable repeat numbers are specific to avian species. Furthermore, sequencing has revealed polymorphisms in MEF2D CAG repeat at both DNA and mRNA levels. Four MEF2D CAG repeat genotypes and 10 MEF2D cDNA variants with different CAG repeat numbers were detected in two duck populations. A t-test showed that the expanded CAG repeat generated significantly longer transcription products (p < 0.05). Association analysis demonstrated positive correlations between the expansion of the CAG repeat and five muscle-related traits. By using protein structure prediction, we suggested that the polymorphisms of the CAG repeat affect protein structures within protein domains. Taken together, these findings reveal that duck MEF2D CAG repeat is a potential functional element with polymorphisms and may cause differences in MEF2D function between duck and other vertebrate species.

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Association of novel polymorphisms in the bovine myocyte enhancer factor 2D (MEF2D) gene with carcass traits of Polish Holstein-Friesian cattle

Cited by 4 publications

References 40 publications

Microsatellite Polymorphism and Prokaryotic Expression of Mef2d in Xingyi Duck

Microsatellite Polymorphism and Prokaryotic Expression of Mef2d in Xingyi Duck

Whole genome resequencing reveals the genetic contribution of Kazakh and Swiss Brown cattle to a population of Xinjiang Brown cattle

Discovery, Characterization, and Functional Study of a NovelMEF2DCAG Repeat in Duck (Anas platyrhynchos)

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