Association of oral bacteria with oral hygiene habits and self‐reported gingival bleeding

Bertelsen, Randi J.; Barrionuevo, Antonio Manuel Pérez; Shigdel, Rajesh; Lie, Stein Atle; Lin, Ho-mu; Real, Francisco Gómez; Ringel–Kulka, Tamar; Åstrøm, Anne Nordrehaug; Svanes, Cecilie

doi:10.1111/jcpe.13644

Cited by 17 publications

(5 citation statements)

References 46 publications

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“…These taxa have been implicated in inflammatory responses associated with oral diseases, including periodontitis and gingivitis. [71][72][73][74] Recent studies of microbiome data from Hispanic adults have shown that the proliferation of genera like Dialister, Fretibacterium, and Mycoplasma correlates with severe periodontal disease, a known risk factor for Alzheimer's disease. 75 Our results align with a previous meta-analysis that examined European and American cohorts, which reported that binge drinking induces a shift in the oral microbiome, characterized by an increased abundance of Dialister and Fretibacterium, and associates genera with AD.…”

Section: Discussionmentioning

confidence: 99%

Differential Patterns of Gut and Oral Microbiomes in Hispanic Individuals with Cognitive Impairment

Wadop,

Vasquez,

Mathews

et al. 2024

Preprint

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Alzheimer's disease and related dementias (ADRD) have been associated with alterations in both oral and gut microbiomes. While extensive research has focused on the role of gut dysbiosis in ADRD, the contribution of the oral microbiome remains relatively understudied. Furthermore, the potential synergistic interactions between oral and gut microbiomes in ADRD pathology are largely unexplored. This study aims to evaluate distinct patterns and potential synergistic effects of oral and gut microbiomes in a cohort of predominantly Hispanic individuals with cognitive impairment (CI) and without cognitive impairment (NC). We conducted 16S rRNA gene sequencing on stool and saliva samples from 32 participants (17 CI, 15 NC; 62.5% female, mean age = 70.4 ± 6.2 years) recruited in San Antonio, Texas, USA. Correlation analysis through MaAslin2 assessed the relationship between participants' clinical measurements (e.g., fasting glucose and blood cholesterol) and their gut and saliva microbial contents. Differential abundance analysis evaluated taxa with significant differences between CI and NC groups, and alpha and beta diversity metrics assessed within-sample and group compositional differences. Our analyses revealed no significant differences between NC and CI groups in fasting glucose or blood cholesterol levels. However, a clear association was observed between gut microbiome composition and levels of fasting glucose and blood cholesterol. While alpha and beta diversity metrics showed no significant differences between CI and NC groups, differential abundance analysis revealed an increased presence of oral genera such as Dialister, Fretibacterium, and Mycoplasma in CI participants. Conversely, CI individuals exhibited a decreased abundance of gut genera, including Shuttleworthia, Holdemania, and Subdoligranulum, which are known for their anti-inflammatory properties. No evidence was found for synergistic contributions between oral and gut microbiomes in the context of ADRD. Our findings suggest that similar to the gut microbiome, the oral microbiome undergoes significant modifications as individuals transition from NC to CI. Notably, the identified oral microbes have been previously associated with periodontal diseases and gingivitis. These results underscore the necessity for further investigations with larger sample sizes to validate our findings and elucidate the complex interplay between oral and gut microbiomes in ADRD pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Differential Patterns of Gut and Oral Microbiomes in Hispanic Individuals with Cognitive Impairment

Wadop,

Vasquez,

Mathews

et al. 2024

Preprint

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“…In our previous paper where we have looked at the association of oral hygiene habit with self-reported gingival bleeding we found that self-reported gingival bleeding was associated with a higher abundance of well-known and novel periodontal pathogens such as Porphyromonas endodontalis, Treponema denticola, and Fretibacterium spp., these bacteria are members of the Red complex [ 29 ]. However, it was observed that the abundance of bacteria belonging to the gram-positive phyla Firmicutes and Actinobacteria was lower in these cases.…”

Section: Discussionmentioning

confidence: 99%

Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian population

et al. 2023

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Background The oral cavity is the gateway to the bacteria community in the lung. Disruption of the symbiotic balance of the oral microbiota has been associated with respiratory diseases. However, little is known about the relationship between oral bacteria and respiratory outcomes in the general population. We aimed to describe the associations between oral bacteria, lung function, and lung inflammation in a community-based population. Methods Oral (gingival) samples were collected concurrently with spirometry tests in 477 adults (47% males, median age 28 years) from the RHINESSA study in Bergen, Norway. Bacterial DNA from the 16S rRNA gene from gingival fluid were sequenced by Illumina®MiSeq. Lung function was measured using spirometry and measurement of fractional exhaled nitric oxide (FeNO) were performed to examine airway inflammation. Differential abundance analysis was performed using ANCOM-BC, adjusting for weight, education, and smoking. Results The abundance of the genera Clostridiales, Achromobacter, Moraxella, Flavitalea and Helicobacter were significantly different among those with low FEV1 (< lower limit of normal (LLN)) as compared to normal FEV1 i.e. ≥ LLN. Twenty-three genera differed in abundance between among those with low FVC < LLN as compared to normal FEV1 ≥ LLN. The abundance of 27 genera from phyla Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria and Sacchribacteria differed significantly between elevated FeNO levels (≥ 50 ppb) compared to FeNO ≤ 25 ppb. Conclusion Oral bacterial composition was significantly different for those with low FEV or FVC as compared to those with normal lung function equal to or higher than LLN. Differential bacterial composition was also observed for elevated FeNO levels.

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“…Although their associations did not reach statistical significance, the wide range of confidence intervals suggests the possibility of their involvement in the pathogenesis of oral diseases, These findings may belong to sample size, which plays an important role in results and the weak facilities during sample collection . Further research is warranted to explore the specific contributions of these bacterial groups and their impact on oral health, like how you live, eat, care for your mouth, get medical help, how old you are, whether you are male or female, Your genetics all work together and affect how the disease gets worse and how bad it gets (Bertelsen, et al,2022) to the unique microenvironment in this area. Gingival crevices contain fluids called gingival crevicular fluid (GCF), which provide bacteria with essential nutrients.…”

Section: Organismmentioning

confidence: 99%

Oral microbiota relationship with and without gingivitis in Iraqi patients

Khalaf,

Hussain,

Saleh

2023

JANS

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Gingivitis is a common oral disease characterized by inflammation of the gum tissues, predominantly caused by microbial dysbiosis. The study aimed to anticipate that the comparative analysis of oral microbiota in Iraqi patients with and without gingivitis would reveal distinct microbial profiles associated with the disease. A cross-sectional study was conducted on Iraqi patients aged 18-65, including males and females. One hundred patients and 50 healthy samples were collected from January to May 2023, diagnosed by Vitek 2 compact system and 16SrRNA. Two hundred and thirty five isolates were obtained form both techniques as four groups:Anaerobic gram-positive (Actinomyces odontolyticus, Actinomyces naeslundii, Streptococcus mutans, Streptococcus anginusom, Streptococcus mitis, Streptococcus oralis, Clostridium sordelli, Group C streptococcus,Streptococcus saliveris, Clostridium histolyticum, Lactobacillus spp., Anaerococcus prevoti, Gemella morbillorum, Turicella Otitidis , Enterococcus casseliflavus, Anaerobic gram-negative(Fusobacterium nucleatum, Vellionela spp., Tannerella forsythia, Fusobacterium mortiifirum, Porphyromonas gingivalis, Prevotella intemedia, Fusobacterium varium, Prevotella disiens, Ggregatibacter actinomycetemcomitans, Enterobacter hormaechei), Facultative gram-positive (Bacillus amyloliquefacieneus,Bacillus atrophaeus, Rothia sp., Bacillus pumilus, Bacillus subtilis, Staphylococcus epidermidis, Bacillus megaterium, Erysipelothrix sp., Staphylococcus aureus, Micrococcus luteus), and Facultative gram-negative bacteria (Pantoea calida, Klebsiella pneumoniae, Pseudomonas putida, Acinetobacter pitti). The anaerobic gram-negative bacteria were the most likely to cause gum diseases. On the other hand the confidence intervals for other group could be attributed to oral disease.Furthermore, these findings can contribute to developing diagnostic and treatment strategies for gingivitis in Iraq.

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Association of oral bacteria with oral hygiene habits and self‐reported gingival bleeding

Cited by 17 publications

References 46 publications

Differential Patterns of Gut and Oral Microbiomes in Hispanic Individuals with Cognitive Impairment

Differential Patterns of Gut and Oral Microbiomes in Hispanic Individuals with Cognitive Impairment

Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian population

Oral microbiota relationship with and without gingivitis in Iraqi patients

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