Association of Paraoxonase-1 Q192R (rs662) Single Nucleotide Variation with Cardiovascular Risk in Coffee Harvesters of Central Colombia

Siller-López, Fernando; Garzón-Castaño, Sandra Catalina; Ramos-Márquez, Martha E.; Hernandez-Cañaveral, Iván

doi:10.1155/2017/6913106

Cited by 11 publications

(6 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This work researched the polymorphisms mentioned (Q192R, L55 M, and C-108T). The results show that the allelic and gene frequencies of these polymorphisms ( Table 3 ) do not differ much from those found in the North American [ 23 ], Latin [ 24 , 25 ], and even Colombian populations [ 26 ].…”

Section: Discussionmentioning

confidence: 69%

Arylesterase activity of paraoxonase 1 (PON1) on HDL3 and HDL2: Relationship with Q192R, C-108T, and L55M polymorphisms

et al. 2021

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

Background Controversy exists regarding the role of the subfractions of high-density lipoproteins (HDL 2 and HDL 3 ) in cardiovascular disease. The functionality of these particles, and their protective role, is due in part to the paraoxonase 1 (PON1) presence in them. The polymorphisms rs662 (Q192R, A/G), rs854560 (L55 M, T/A), and rs705379 (C-108T) of the PON1 gene have been related to enzyme activity and, with the anti-oxidative capacity of the HDL. The objective was to determine the arylesterase PON1 activity in HDL 3 and HDL 2 and its relationship with the polymorphisms mentioned, in a young population. Methods The polymorphisms were determined through mini-sequencing (SnaPshot). The HDL subpopulations were separated via ionic precipitation, cholesterol was measured with enzymatic methods, and PON1 activity was measured through spectrophotometry. Results The results show that the PON1 polymorphisms do not influence the cholesterol in the HDL. A variation between 40.02 and 43.9 mg/dL was in all the polymorphisms without significant differences. Additionally, PON1 activity in the HDL 3 subfractions was greater (62.83 ± 20 kU/L) than with HDL 2 (35.8 ± 20.8 kU/L) in the whole population and in all the polymorphisms (p < 0.001), and it was independent of the polymorphism and differential arylesterase activity in the Q192R polymorphism (QQ > QR > RR). Thus, 115.90 ± 30.7, 88.78 ± 21.3, 65.29 ± 10.2, respectively, for total HDL, with identical behavior for HDL 3 and HDL 2 . Conclusions PON1 polymorphisms do not influence the HDL -c , and the PON activity is greater in the HDL 3 than in the HDL 2 , independent of the polymorphism, but it is necessary to delve into the functionality of these findings in different populations.

show abstract

Section: Discussionmentioning

confidence: 69%

Arylesterase activity of paraoxonase 1 (PON1) on HDL3 and HDL2: Relationship with Q192R, C-108T, and L55M polymorphisms

et al. 2021

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

show abstract

“…PON1 rs662 gene polymorphism is involved in modulating the PON1 enzyme 23 . The R allele variant is implicated in the less active isoform of PON1 against lipoprotein oxidation, and higher ox-LDL, triglycerides, and LDL-C, which lead to an increased risk of vascular dysfunction 24 , 25 . Moreover, the inter-relationship between the PON1 and PON2 genes may affect glycaemic control in patients with diabetic retinopathy 26 .…”

Section: Discussionmentioning

confidence: 99%

Association between PON1-rs662 Gene Polymorphism and Diabetic Retinopathy in Population of the Qom, Iran

Bidgoli,

Colagar,

Tafrihi

et al. 2023

AJMB

View full text Add to dashboard Cite

Background: Diabetic retinopathy is the most severe diabetic microvascular complication that causes changes in the vessel wall. One of the genes involved in this disease is PON1, which encodes paraoxanase1 protein in liver and kidney. It might regulate inflammatory and microvascular responses to the disease. The rs662 T>C is one of the single nucleotide polymorphisms of this gene that changes glutamine to arginine at position 192. Methods: In this study, 300 samples were collected, including 100 healthy and 100 diabetics without retinopathy, and 100 diabetics retinopathies were studied and their age range was from 30 to 80 years. Then 2.5 ml of blood was collected from all relevant individuals in tubes containing EDTANa2. This polymorphism was examined by tetra-ARMS PCR. Results: Results showed that there is no significant correlation between genotypes and alleles related to PON1 and Diabetes (CC genotype: p=0.609; C allele: p=0.228). On the other hand, an association was observed between PON1 and diabetic retinopathy (CT+CC genotype: p<0.001; CT allele: p<0.001). Considering that the Polyphen database examined the changes caused by replacing the amino acid arginine instead of glutamine at position 129 on the protein, it does not consider these changes dangerous and has introduced this polymorphism as benign. Conclusion: Based on the findings of this study, the rs662 locus could be considered as one of the molecular markers in future research.

show abstract

“…The R allele variant has been associated with a less active isoform of PON1 against lipoprotein oxidation, resulting in an enhanced risk of vascular disease [40]. A meta-analysis study found that the R allele gene variant is significantly associated with increased levels of ox-LDL, triglycerides, total cholesterol, and LDL-C, which may lead to a higher risk of CAD [22].…”

Section: Discussionmentioning

confidence: 99%

Association between PON1 rs662 gene polymorphism and serum paraoxonase1 level in coronary artery disease patients in Northern India

Kumar

Saini

Kaur

et al. 2021

Egypt J Med Hum Genet

View full text Add to dashboard Cite

Background Coronary artery disease (CAD) is a leading cause of morbidity and mortality, with a shifting trend towards the younger population. Paraoxonase1 (PON1) is a glycoprotein enzyme associated with high-density lipoprotein (HDL) particles in the blood. It has the ability to protect against lipid oxidation, thereby reducing the risk of atherogenesis. PON1 rs662 gene polymorphism may affect serum PON1 levels as well as its activity and may have a significant role in the pathogenesis of CAD. The present study was conducted to identify the association of PON1 rs662 gene polymorphism with serum PON1 levels in CAD patients in the North Indian population. This case–control study included 71 angiography-proven CAD patients (with > 50% luminal stenosis in one or more coronary arteries) and 71 controls (with < 50% luminal obstruction in angiography). PON1 rs662 gene polymorphism was studied using PCR and RFLP under the standardized protocol. Serum PON1 levels were estimated by ELISA. Results The serum PON1 level was significantly lower in the CAD group than in the controls (7.79 ± 3.16 vs. 10.79 ± 3.19 ng/mL; p < 0.0001). Logistic regression analysis showed that homozygous GG genotype of PON1 rs662 SNP has ninefold increased risk of developing CAD in an Indian population (OR = 9.0, 95%CI 2.79–29.06, p = 0.0002). A significantly higher frequency of G allele was also observed in CAD patients than in controls (OR 2.64, 95%CI 1.61–4.33, p = 0.001). Conclusions The reduced serum PON1 level is associated with CAD. PON1 rs662 gene polymorphism is significantly associated with CAD susceptibility in the North Indian population.

show abstract

Association of Paraoxonase-1 Q192R (rs662) Single Nucleotide Variation with Cardiovascular Risk in Coffee Harvesters of Central Colombia

Cited by 11 publications

References 48 publications

Arylesterase activity of paraoxonase 1 (PON1) on HDL3 and HDL2: Relationship with Q192R, C-108T, and L55M polymorphisms

Arylesterase activity of paraoxonase 1 (PON1) on HDL3 and HDL2: Relationship with Q192R, C-108T, and L55M polymorphisms

Association between PON1-rs662 Gene Polymorphism and Diabetic Retinopathy in Population of the Qom, Iran

Association between PON1 rs662 gene polymorphism and serum paraoxonase1 level in coronary artery disease patients in Northern India

Contact Info

Product

Resources

About