Association of paraoxonase polymorphisms with carotid artery atherosclerosis in essential hypertension patients

Han, Lei; Xu, Xiaoyu; Xue, L.; Ma, Jinzhao

doi:10.4238/2013.october.30.2

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…21 PON1 was also significantly differentially expressed between HF and non-HF patients and significantly associated with HFrEF. Studies in two Japanese patient cohorts have revealed that the presence of certain alleles of PON1 increase the risk of carotid artery atherosclerotic disease 22 ; however, the clinical significance of PON1 activity in cardiovascular conditions remains controversial. Some studies have associated low baseline PON1 activity with increased severity of coronary artery disease, while other studies report an association between high baseline PON1 activity and coronary artery disease severity.…”

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confidence: 99%

Multiplexed measurement of candidate blood protein biomarkers of heart failure

et al. 2021

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Aims There is a critical need for better biomarkers so that heart failure can be diagnosed at an earlier stage and with greater accuracy. The purpose of this study was to design a robust mass spectrometry (MS)-based assay for the simultaneous measurement of a panel of 35 candidate protein biomarkers of heart failure, in blood. The overall aim was to evaluate the potential clinical utility of this biomarker panel for prediction of heart failure in a cohort of 500 patients. Methods and results Multiple reaction monitoring (MRM) MS assays were designed with Skyline and Spectrum Mill PeptideSelector software and developed using nanoflow reverse phase C18 chromatographic Chip Cube-based separation, coupled to a 6460 triple quadrupole mass spectrometer. Optimized MRM assays were applied, in a sample-blinded manner, to serum samples from a cohort of 500 patients with heart failure and non-heart failure (non-HF) controls who had cardiovascular risk factors. Both heart failure with reduced ejection fraction (HFrEF) patients and heart failure with preserved ejection fraction (HFpEF) patients were included in the study. Peptides for the Apolipoprotein AI (APOA1) protein were the most significantly differentially expressed between non-HF and heart failure patients (P = 0.013 and P = 0.046). Four proteins were significantly differentially expressed between non-HF and the specific subtypes of HF (HFrEF and HFpEF); Leucine-rich-alpha-2glycoprotein (LRG1, P < 0.001), zinc-alpha-2-glycoprotein (P = 0.005), serum paraoxanse/arylesterase (P = 0.013), and APOA1 (P = 0.038). A statistical model found that combined measurements of the candidate biomarkers in addition to BNP were capable of correctly predicting heart failure with 83.17% accuracy and an area under the curve (AUC) of 0.90. This was a notable improvement on predictive capacity of BNP measurements alone, which achieved 77.1% accuracy and an AUC of 0.86 (P = 0.005). The protein peptides for LRG1, which contributed most significantly to model performance, were significantly associated with future new onset HF in the non-HF cohort [Peptide 1: odds ratio (OR) 2.345 95% confidence interval (CI) (1.456-3.775) P = 0.000; peptide 2: OR 2.264 95% CI (1.422-3.605), P = 0.001]. Conclusions This study has highlighted a number of promising candidate biomarkers for (i) diagnosis of heart failure and subtypes of heart failure and (ii) prediction of future new onset heart failure in patients with cardiovascular risk factors. Furthermore, this study demonstrates that multiplexed measurement of a combined biomarker signature that includes BNP is a more accurate predictor of heart failure than BNP alone.

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Multiplexed measurement of candidate blood protein biomarkers of heart failure

et al. 2021

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“…As expected, correlation factor in negative acute phase reactant PON1-ARE activity was found to be negative, depending on TNFα in the studied subjects. It has been reported that inflammatory conditions result in PON1 enzymatic activity exhaustion and down regulation of hepatic PON1 mRNA levels [25,26,41]. …”

Section: Resultsmentioning

confidence: 99%

Acute Phase Response: Implication in ST-segment Elevation Myocardial Infarction

Eren

Elli̇dağ

Yılmaz

et al. 2014

TOBIOCJ

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We aimed to investigate the relation between serum inflammatory markers, 25OHvit-D3 and oxidative stress markers, namely paraoxonase1-arylesterase (PON1-ARE), total antioxidant status (TAS) and total oxidant status (TOS) in 30 male patients with ST–elevation myocardial infarction(STEMI) . There was negative correlation between tumor necrosis factor alpha and ARE; positive correlations between serum amyloid A(SAA) and oxidative stress index, SAA and TOS, 25OHvit-D3 and ARE. There was no statistically significant correlation between inflammation makers, oxidative stress markers and Gensini score. The main finding of our study was the tendency of inflammation markers, and oxidative stress markers, to change in relatively clear opposite directions in STEMI.

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Identification of proteins found to be significantly altered when comparing the serum proteome from Multiple Myeloma patients with varying degrees of bone disease

et al. 2014

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BackgroundBone destruction is a feature of multiple myeloma, characterised by osteolytic bone destruction due to increased osteoclast activity and suppressed or absent osteoblast activity. Almost all multiple myeloma patients develop osteolytic bone lesions associated with severe and debilitating bone pain, pathologic fractures, hypercalcemia, and spinal cord compression, as well as increased mortality. Biomarkers of bone remodelling are used to identify disease characteristics that can help select the optimal management of patients. However, more accurate biomarkers are needed to effectively mirror the dynamics of bone disease activity.ResultsA label-free mass spectrometry-based strategy was employed for discovery phase analysis of fractionated patient serum samples associated with no or high bone disease. A number of proteins were identified which were statistically significantly correlated with bone disease, including enzymes, extracellular matrix glycoproteins, and components of the complement system.ConclusionsEnzyme-linked immunosorbent assay of complement C4 and serum paraoxonase/arylesterase 1 indicated that these proteins were associated with high bone disease in a larger independent cohort of patient samples. These biomolecules may therefore be clinically useful in assessing the extent of bone disease.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-904) contains supplementary material, which is available to authorized users.

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Association of paraoxonase polymorphisms with carotid artery atherosclerosis in essential hypertension patients

Cited by 4 publications

References 36 publications

Multiplexed measurement of candidate blood protein biomarkers of heart failure

Multiplexed measurement of candidate blood protein biomarkers of heart failure

Acute Phase Response: Implication in ST-segment Elevation Myocardial Infarction

Identification of proteins found to be significantly altered when comparing the serum proteome from Multiple Myeloma patients with varying degrees of bone disease

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