Association of Pectolytic Strains of<i>Xanthomonas campestris</i>with Soft Rots of Fruits and Vegetables at Retail Markets

Liao, Ching-Hsing

doi:10.1094/phyto-77-418

Cited by 43 publications

(22 citation statements)

References 5 publications

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“…Th erefore they are generally more susceptible to infection by natural pathogens or even to attack by bacteria that normally do not cause diseases of plants growing in the fi eld (Liao and Wells 1987). King and Bolin (1989) indicated the increase of microbial populations of lettuce under storage conditions which makes our results surprising since we found that the spectrum of identifi ed bacterial pathogens was lower in the second sampling than in the fi rst one.…”

Section: Discussionmentioning

confidence: 60%

Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Eichmeier¹,

Peňázová²,

Pecenka³

et al. 2016

Journal of Plant Protection Research

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Twenty-six cabbage heads stored under typical conditions in a storage hall in Moravia, Czech Republic, were tested for the presence of bacteria by the method of isolation from three diff erent parts of the cabbage heads. Isolations were carried out from stalks, inner and superfi cial leaves. Two samplings were done; in November 2015 and February 2016. Bacterial cultures were sequenced in the part of 16S rRNA region; bacteria were identifi ed according to the sequences obtained. Th e most prevalent bacteria were of the genus Pseudomonas. Genera: Klebsiella, Erwinia, Pantoea, Bacillus were also identifi ed. Th e results provided an interesting insight into the bacterial spectrum in stored cabbage heads and their dynamics during storage. Th e nucleotide sequences which were found were saved in GenBank/NCBI under accession numbers KX160104-KX160145.

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Section: Discussionmentioning

confidence: 60%

Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Eichmeier¹,

Peňázová²,

Pecenka³

et al. 2016

Journal of Plant Protection Research

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“…oryzae (Wakimoto, 1960;Goto, 1965). The early Xpl0 genes are transcribed by the host polymerase, whereas the late Xpl0 genes are transcribed by the Xpl0 RNA polymerase (Liao and Kuo, 1986;Liao et al, 1987). Their host range is restricted to X. o. pv.…”

Section: Phagesmentioning

confidence: 99%

Xanthomonas

1993

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“…vesicatoria induce soft rot symptoms and cause problems on postharvest vegetables and fruits. These pathogenic bacteria are capable of producing pectolytic enzymes and causing maceration of potato tubers and green pepper (Starr and Nasuno, 1967;Liao and Wells, 1987;Beaulieu et al, 1991). The cotton blight pathogen, X. campestris pv.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of pectolytic enzyme genes in Xanthomonas citri subsp. citri and demonstration that pectate lyase Pel3 is required for the formation of citrus canker

Chang

Deng

Huang

et al. 2016

Microbiological Research

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Bacterial canker, caused by Xanthomonas citri subsp. citri (Xcc), is one of the most destructive diseases of citrus. The pectolytic enzymes produced by phytobacteria are important virulence factors involved in tissue maceration, electrolyte loss and cell death of host plants. In this study, the promoter activity of the pectolytic enzyme genes pel1, pel2, pel3, pglA, and peh-1 were investigated in Xcc XW19 strain using the β-glucuronidase (GUS) gene as a reporter. GUS activity expressed under the control of the pel1, pel3, pglA, and peh-1 gene promoters positively correlated with bacterial growth. These gene promoters displayed high GUS activity in the presence of sodium polypectate. In addition, the four genes were induced in XVM2 minimal medium. However, only pel1 was subjected to catabolite repression by glucose. GUS activity was significantly enhanced in the XW19-derived reporter strains after they were inoculated into the leaves of Mexican lime and grapefruit, suggesting the involvement of the pel1, pel3, pglA, and peh-1 genes in XW19 pathogenesis. The pel3 promoter produced the highest GUS activity under all test conditions, whereas no GUS activity was detected using the pel2 promoter in vitro and in planta. In comparison with wild type Xcc, a pel3 mutant generated from Xcc XW19 using unmarked mutagenesis displayed reduced growth and induced smaller canker lesions on the leaves of Mexican lime, demonstrating that Pel3 of Xcc strain XW19 is a virulence factor.

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Association of Pectolytic Strains ofXanthomonas campestriswith Soft Rots of Fruits and Vegetables at Retail Markets

Abstract: We thank J. E. Butterfield and R. Oseredczuk for help in collecting plant specimens and preparing culture media, respectively.

Cited by 43 publications

References 5 publications

Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Xanthomonas

Differential expression of pectolytic enzyme genes in Xanthomonas citri subsp. citri and demonstration that pectate lyase Pel3 is required for the formation of citrus canker

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