Association of periodontitis with GSTM1/GSTT1-null variants—A pilot study

Concolino, Paola; Cecchetti, F; D'Autilia, Caterina; Santonocito, Concetta; Stasio, Enrico Di; Zuppi, Cecilia; Arcuri, C; Deli, Giorgio; Giardina, Bruno; Capoluongo, Ettore; Ameglio, Franco

doi:10.1016/j.clinbiochem.2007.04.012

Cited by 22 publications

(14 citation statements)

References 36 publications

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“…Although GST polymorphisms were not previously evaluated as potential AP risk factors, it is well known that GST null genotypes are associated with decreased enzymatic activity and poor antioxidant protection (Whalen & Boyer 1998), and that disturbed antioxidant activity significantly promotes oxidative stress in AP (Hernández‐Ríos et al 2017). In accordance with the finding of the present study, Concolino et al (2007) and Camargo Ortega et al (2014) have reported that subjects with null and double‐null GSTM1 and GSTT1 genotypes have a higher risk of developing aggressive and chronic periodontitis. This study is also in agreement with previous reports on GST deletion polymorphisms as significant modulators of temporomandibular disorders (Milosevic et al 2015), pre‐eclampsia (Sljivancanin Jakovljevic et al 2019) and atherogenesis (Grubisa et al 2018) risk in Serbian populations.…”

Section: Discussionsupporting

confidence: 93%

Association of polymorphisms in TNF‐α, IL‐1β, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?

et al. 2020

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Aim To investigate the possible association between TNFα (−308 G/A) and IL‐1β (−511 C/T) single nucleotide polymorphisms (SNPs) and GSTT and GSTM deletion polymorphisms and risk of apical periodontitis (AP) development, and determine the association of different genotypes with the presence of herpesviral infection in AP. Methodology The study included 120 periapical lesions and 200 control samples. Gene polymorphism analysis was performed using either polymerase chain reaction (PCR) or PCR/ restriction fragment length polymorphism (RFLP). Relative gene expression of TNF‐α and IL‐1β was analysed using reverse transcriptase – real‐time PCR. The presence of Epstein–Barr virus (EBV) and human cytomegalovirus (HCMV) was assessed by nested PCR. Chi‐square and Fisher’s exact tests and logistic regression analyses were done for polymorphisms, whilst Mann–Whitney U‐test was performed for the expression analysis. The expected frequency of variants was analysed by the Hardy–Weinberg equilibrium test. Results TNF‐α (−308 G/A) SNP increased AP susceptibility for heterozygous (odds ratio (OR) = 1.72, 95% confidence interval (CI) = 1.06–2.80, P = 0.027) and homozygous (OR = 8.55, 95% CI = 1.77–41.36, P < 0.001) carriers of the variant A allele. On the other hand, IL‐1β (−511 C/T) polymorphism exerted a protective effect both in heterozygotes (OR = 0.540, 95% CI = 0.332–0.880, P = 0.013) and homozygotes (OR = 0.114, 95% CI = 0.026–0.501, P < 0.001). In addition, GSTM1 and GSTT1 null genotypes separately, as well as concomitantly, were associated with an increased risk for AP development (P < 0.001). The null GSTT1 genotype increased approximately twice the risk of Epstein–Barr infection (EBV) in AP (OR = 2.17, 95% CI = 1–4.71, P = 0.048), whilst TNF‐α SNP decreased it, both in heterozygotes (OR = 0.20, 95% CI = 0.08–0.48, P < 0.001) and AA homozygotes (OR = 0.07, 95% CI = 0.01–0.37, P = 0.001). Conclusions GSTM and GSTT deletion polymorphisms, as well as TNFα (−308 G/A) SNP, are associated with increased risk, whereas IL‐1β (−511 C/T) polymorphism decreases the risk of AP development. GSTT and TNFα polymorphisms also appear to modulate the risk of EBV infection in Serbian patients with AP.

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Section: Discussionsupporting

confidence: 93%

Association of polymorphisms in TNF‐α, IL‐1β, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?

et al. 2020

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“…Online Cochrane library database revealed one controlled clinical trial when searched for “glutathione and periodontal.” Pertinent articles in English language on the topic and abstracts of relevant papers were scrutinized thoroughly, and finally papers pertaining to the topic were included after excluding the duplicates [Tables 1 - 5 ]. [ 4 5 28 29 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 …”

Section: Methodsmentioning

confidence: 99%

The antioxidant master glutathione and periodontal health

Bains

2015

Dent Res J

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Glutathione, considered to be the master antioxidant (AO), is the most-important redox regulator that controls inflammatory processes, and thus damage to the periodontium. Periodontitis patients have reduced total AO capacity in whole saliva, and lower concentrations of reduced glutathione (GSH) in serum and gingival crevicular fluid, and periodontal therapy restores the redox balance. Therapeutic considerations for the adjunctive use of glutathione in management of periodontitis, in limiting the tissue damage associated with oxidative stress, and enhancing wound healing cannot be underestimated, but need to be evaluated further through multi-centered randomized controlled trials.

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“…The GSTM1 and GSTT1 genotypes were determined as previously described without any modification [ 20 , 21 ]. Briefly, the β-globin primer pair (sense: 5'-CAACTTCATCCACGTTCACC-3' and antisense: 5'-GAAGAGCCAAGGACAGGTAC-3'), which had not been deleted, was used as an internal control.…”

Section: Methodsmentioning

confidence: 99%

The effect ofglutathione S-transferase M1andT1polymorphisms on blood pressure, blood glucose, and lipid profiles following the supplementation of kale (Brassica oleracea acephala) juice in South Korean subclinical hypertensive patients

Han

Lee

Kim³

et al. 2015

Nutr Res Pract

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BACKGROUND/OBJECTIVESGlutathione S-transferase (GST) forms a multigene family of phase II detoxification enzymes which are involved in the detoxification of reactive oxygen species. This study examines whether daily supplementation of kale juice can modulate blood pressure (BP), levels of lipid profiles, and blood glucose, and whether this modulation could be affected by the GSTM1 and GSTT1 polymorphisms.SUBJECTS/METHODS84 subclinical hypertensive patients showing systolic BP over 130 mmHg or diastolic BP over 85 mmHg received 300 ml/day of kale juice for 6 weeks, and blood samples were collected on 0-week and 6-week in order to evaluate plasma lipid profiles (total cholesterol, triglyceride, HDL-cholesterol, and LDL-cholesterol) and blood glucose.RESULTSSystolic and diastolic blood pressure was significantly decreased in all patients regardless of their GSTM1 or GSTT1 polymorphisms after kale juice supplementation. Blood glucose level was decreased only in the GSTM1-present genotype, and plasma lipid profiles showed no difference in both the GSTM1-null and GSTM1-present genotypes. In the case of GSTT1, on the other hand, plasma HDL-C was increased and LDL-C was decreased only in the GSTT1-present type, while blood glucose was decreased only in the GSTT1-null genotype.CONCLUSIONSThese findings suggest that the supplementation of kale juice affected blood pressure, lipid profiles, and blood glucose in subclinical hypertensive patients depending on their GST genetic polymorphisms, and the improvement of lipid profiles was mainly greater in the GSTT1-present genotype and the decrease of blood glucose was greater in the GSTM1-present or GSTT1-null genotypes.

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Association of periodontitis with GSTM1/GSTT1-null variants—A pilot study

Cited by 22 publications

References 36 publications

Association of polymorphisms in TNF‐α, IL‐1β, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?

Association of polymorphisms in TNF‐α, IL‐1β, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?

The antioxidant master glutathione and periodontal health

The effect ofglutathione S-transferase M1andT1polymorphisms on blood pressure, blood glucose, and lipid profiles following the supplementation of kale (Brassica oleracea acephala) juice in South Korean subclinical hypertensive patients

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