Association of Plasma IL-32 Levels and Gene Polymorphisms with Systemic Lupus Erythematosus in Chinese Han Population

Abstract

Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease. IL-32, a secreted protein, has been reported to be associated with several autoimmune diseases. Our preliminary experiment showed different plasma IL-32 levels than that mentioned in a published report on the same population. In order to elucidate the correlation between IL-32 and SLE, we determined the plasma level and two single nucleotide polymorphisms (SNPs) of IL-32 in 152 patients with SLE and 310 healthy controls and analyzed the relationship based on the clinical parameters. The results showed that plasma IL-32 levels in patients with SLE were markedly lower than that in the healthy controls. In the SLE group, patients with detectable IL-32 presented low serum C3 concentrations. Further studies indicated that the rs28372698 SNP was associated with the susceptibility to SLE. Taken together, our results suggested that IL-32 could possibly be a candidate marker to monitor SLE disease stability and screening in future.

Elevated Gene Expression of Interleukin-32 Isoforms Alpha, Beta, Gamma, and Delta in the Peripheral Blood of Chronic Psoriatic Patients

“…In recent decades, abnormalities in the expression and production of IL-32 have been identified in many human disorders such as rheumatoid arthritis, various cancers, pulmonary tuberculosis, atopic dermatitis, leishmaniasis, etc. [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. Importantly, an elevated IL-32 level was found in the sera of patients with atopic dermatitis, asthmatic patients, systemic lupus erythematosus, etc.…”

“…Importantly, an elevated IL-32 level was found in the sera of patients with atopic dermatitis, asthmatic patients, systemic lupus erythematosus, etc. [ 27 , 28 , 30 ], indicating that IL-32 was produced by blood cells. However, the role of IL-32 in the peripheral blood has never been investigated in psoriasis.…”

“…The proinflammatory role of IL-32 has been well reported in several disorders including rheumatoid arthritis, cancers, pulmonary tuberculosis, etc. [ 21 , 22 , 23 , 24 , 25 , 26 , 27 ], and also in many skin disorders such as atopic dermatitis, hidradenitis suppurativa, leishmaniasis, and systematic lupus erythematosus [ 28 , 29 , 30 ]. Most importantly, elevated IL-32 has also been reported in skin biopsies of psoriasis patients [ 31 ].…”

Interleukin-32: its role in asthma and potential as a therapeutic agent

“… SLE ELISA (R&D Systems, USA) Plasma: healthy controls, 94.4 (40.2–233.7); SLE, 34.7 (15.5–140.5). SLE patients had lower levels of IL-32 than healthy controls [ 63 ]. This decreased level may be associated with drug treatment and the chemotherapy-related bone marrow cytotoxicity.…”

Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity

“…Controls were recruited from health check volunteers at West China Second University Hospital of Sichuan University, Chengdu, China as described in a previous study. [ 11 ] This study was approved by the ethics committees of West China Hospital and West China Second Hospital of Sichuan University. All subjects signed the informed consent forms.…”