Association of polyamines to different parts of various plant species

Felix, Hansruedi; Harr, Jost

doi:10.1111/j.1399-3054.1987.tb04337.x

Cited by 37 publications

(22 citation statements)

References 15 publications

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“…The effect of exogenous PAs or their biosynthetic inhibitors has been commonly employed to evaluate the role of PAs in various plant and developmental processes (Egea-Cortines and Mizrahi 1991 ). Felix and Harr (1987) found that the inhibitors neither deplete PA levels nor inhibit germination of seeds of sugar maple (Acer saccharum).…”

Section: Discussionmentioning

confidence: 99%

Proteins and polyamines during dormancy breaking of European beech (Fagus sylvatica L.) seeds

2003

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The studies were carried out on Fagus svlvatica seeds during stratification and their germination. After imbibition beechnuts were subjected to cold (3 'C -temperature which breaks dormancy) or warm (15 C -temperature unable to break dormancy) stratification and alternatively were treated with polyamine synthesis inhibitors: canavanine and DFMO (difluoromethylornithine). After cold stratification in embryo axes we found (using 2-D electrophoresis) about 150 new proteins absent in dry seeds. Exogenous spermidine increased the protein synthesis, percent of germinated seeds and accelerated breaking of dormancy. In contrast, canavanine and DFMO decreased dynamic of protein synthesis, quantity of proteins probably synthesised de novo, and percent of germinated seeds. The maximum of polyamine content in embryo axes during cold stratification preceded such the maximum during warm stratification. Irrespective of the influence of PAs and inhi bitors of PA synthesis, the comparison of electrophoregrams and autoradiograms showed that different groups synthesised de novo appeared after different periods of cold stratification. Probably the part of this protein is associated with Fagus syh'atica seeds dormancy breaking.

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Section: Discussionmentioning

confidence: 99%

Proteins and polyamines during dormancy breaking of European beech (Fagus sylvatica L.) seeds

2003

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“…Ainda segundo esses mesmos autores é possível que, como as giberelinas, as poliaminas possam estar envolvidas tanto na divisão quanto na elongação celular. Felix & Harr (1983) observaram que o conteúdo total de poliaminas é maior em sementes embebidas do que em sementes secas. Isso apoia a hipótese de que o acúmulo de putrescina (a poliamina mais abundante) observado no embrião durante a embebição das sementes é devido à sua síntese líquida e, provavelmente, está relacionado às numerosas divisões celulares que ocorrem no início da germinação.…”

Section: 6 ------------------------------------------------------% Dunclassified

Estresse hídrico e reguladores de crescimento na germinação de sementes de canafístula

Botelho

Gualtieri

2001

Sci. agric. (Piracicaba, Braz.)

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RESUMO: Sementes selecionadas escarificadas de canafístula foram submetidas a estresses hídricos simulados, sob 27°C, com ou sem a adição de poliaminas e giberelina. Os valores de porcentagem e velocidade de germinação obtidos com os testes realizados indicaram que as sementes são resistentes ao estresse hídrico, e a adição de putrescina e espermidina nas concentrações de 5 e 10mM aumenta a performance sob estresse hídrico. O uso de giberelina permite a ampliação do limite máximo de tolerância ao estresse hídrico. Palavras-chave: Peltophorum dubium, PEG 6000, espermidina, giberelina, putrescina GROWTH REGULATORS AND WATER STRESS IN CANAFISTULA SEED GERMINATIONABSTRACT: Scarified selected seeds of the wood tree species 'canafístula' were submitted to water stress at 27 o C, with and without addition of putrescine and spermidine at concentrations of 5 and 10mM and gibberellin 0.1mM. The values of rate and germination percentage of all the experiments indicated that the seeds are resistant to water stress. When putrescine and spermidine were added to the germination media the performance under stress increased significantly. Gibberellin extended the maximal tolerance limit to water stress. Key words: Peltophorum dubium, PEG6000, gibberelin, spermidine, putrescine INTRODUÇÃOPeltophorum dubium Spreng (Taubert) (canafístula) pertence à família Fabaceae sub família Mimosoidae. É uma espécie vegetal decídua, heliófita, pioneira, característica da floresta latifoliada semidecídua da Bacia do Paraná, ocorrendo preferencialmente em solos argilosos, úmidos e profundos de beira de rios, tanto na floresta primária densa como em formações secundárias. Apresenta dispersão ampla e abundante, principalmente nas áreas próximas ao grande rio. A madeira é empregada na construção civil, marcenaria, tanoaria, carrocerias, dormentes, serviços de torno, etc. A árvore é ornamental, proporciona ótima sombra quando isolada, podendo ser empregada com sucesso no paisagismo. Como planta rústica e de rápido crescimento, é adequada para a composição de reflorestamentos mistos de áreas degradadas de preservação permanente (Lorenzi, 1992).A água é o fator iniciante da germinação e está envolvida direta e indiretamente em todas as demais etapas do metabolismo germinativo. Sua participação é decisiva nas reações enzimáticas, na solubilização e transporte de metabólitos, como reagente na digestão hidrolítica de tecidos de reserva da semente. Potenciais osmóticos muito negativos atrasam e diminuem a porcentagem de germinação. O grau mínimo de umidade a ser atingido pela semente para que a germinação ocorra, depende de sua composição química e da permeabilidade do tegumento. (Bradford, 1995).Em condições de laboratório, são realizados estudos utilizando soluções aquosas de com diferentes potenciais osmóticos para umedecer o substrato de germinação, procurando simular as condições de de estresse hídrico no solo (Hardegree & Emmerich, 1994). O polietileno glicol é um agente osmótico utilizado nesses estudos; é quimicamente inertes, atóxico para as semen...

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“…Polyamines accumulate in relatively high quantities in resting seeds (Felix and Harr 1987). The polyamines accumulated in embryos of Avena fatua and Oryza sativa, but their content in endosperms was low.…”

Section: Introductionmentioning

confidence: 99%

Response of alfalfa to putrescine treatment under drought stress

Zeid¹,

Shedeed²

2006

Biologia plant.

172

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Alfalfa (Medicago sativa L. cv. Siwa 1) seeds were germinated in polyethylene glycol (PEG 4000) of different concentrations and with or without putrescine. The decrease in water potential of the PEG solution reduced germination rate, germination percentage, and growth criteria (e.g., hypocotyl length, fresh and dry masses of shoot and root), while the root length was increased. The decrease in water potential also reduced the contents of total soluble and reducing sugars, and proteins, and activities of α-and β-amylases and invertase, while increased protease activity. Putrescine treatment improved germination and all growth criteria and increased the activity of the hydrolytic enzymes except protease. In a pot experiment, drought stress was imposed by decreasing the soil moisture. Growth criteria, contents of proteins, chlorophyll a, b and carotenoids, as well as Hill reaction activity decreased while the hydrolytic enzyme activity and total soluble and reducing sugar contents increased under drought stress. Putrescine treatment decreased the activity of the hydrolytic enzymes and increased the polysaccharide, protein and photosynthetic pigment contents, and Hill reaction activity.

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Association of polyamines to different parts of various plant species

Cited by 37 publications

References 15 publications

Proteins and polyamines during dormancy breaking of European beech (Fagus sylvatica L.) seeds

Proteins and polyamines during dormancy breaking of European beech (Fagus sylvatica L.) seeds

Estresse hídrico e reguladores de crescimento na germinação de sementes de canafístula

Response of alfalfa to putrescine treatment under drought stress

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