Association of polyethylene friction and thermal unfolding of interfacial albumin molecules

Fang, Hsu Wei; Shih, Meng Lin; Zhao, Jian; Huang, Huei Ting; Lin, Hung‐Yi; Liu, Hsuan Liang; Chang, Chih Hung; Yang, Charng Bin; Liu, Hwa Chang

doi:10.1016/j.apsusc.2007.02.016

Cited by 12 publications

(6 citation statements)

References 38 publications

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“…In the case of overloaded joints, uneven abrasion conditions and surface replacement implants, the increased temperature increases protein degradation and accelerates abrasion [42]. The most common protein in SF is human serum albumin (HSA), which plays an important role in the lubrication of the cartilage, thereby protecting the joint cartilage surface from the abrasion [43]. The model, investigated under our experimental conditions by differential scanning calorimetry (DSC), proved to be a wellused method in case of healthy, degenerative, and contagious arthritis, for the reproducible detection and quantification of temperature-dependent denaturation of protein content in SF.…”

Section: Discussionmentioning

confidence: 99%

Investigation of protein content of synovial fluids with DSC in different arthritides

Wiegand

Bűcs

Dandé

et al. 2019

J Therm Anal Calorim

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In the case of joint diseases, as a result of the process induced by the pathophysiological factors, the small articular structures that have pilled result in a qualitative and quantitative modification of the composition of the synovial fluid (SF). The analysis of this fluid offers a unique opportunity to study the joint diseases. In our study, 28 cases of acute knee joint meniscus injuries, transient knee joint synovitis, or degenerative knee joint destruction were studied. Following the collection and storage of SF, removed on the basis of the sample collection protocol, they were denatured in a SETARAM Micro DSC-II calorimeter with a scanning rate 0.3 K min -1 in the 20-90°C temperature range. 4-4 samples from the ''normal'' SF batch were inoculated by two different strains of bacteria [Staphylococcus aureus and Staphylococcus epidermidis] with 10 -3 colony-forming unit. Thermoanalytical changes of the proteins found in the SF were studied by a SETARAM Micro DSC-II, in the laboratory of the Biophysical Institute. The maximum denaturation temperature of the proteins being in the SF with different severity varied according to the criterion of the radiological scaling of osteoarthritis made by the Kellgren-Lawrence criterion. The decomposition of the scans showed the degree of the damage in case of SF as well as in the inoculated samples. Knowing the denaturing properties of temperature-dependent protein molecules in SF, DSC can be used to design and develop intraarticular lubricant additives that effectively enhance protein stability and contribute to a reduction in joint wear.

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Section: Discussionmentioning

confidence: 99%

Investigation of protein content of synovial fluids with DSC in different arthritides

Wiegand

Bűcs

Dandé

et al. 2019

J Therm Anal Calorim

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“…Apart from water, proteins count for the largest fraction of constituents in SF. From the tribological perspective, proteins have been considered to play a role in the boundary lubrication process in natural human joints and may also be of importance to artificial joints 6, 25, 39…”

Section: Discussionmentioning

confidence: 99%

“…DSC, a frequently used technique to study the thermal stability of proteins, allowed data to be obtained on the calorimetric heat or enthalpic changes (Δ H ) and entropic changes (Δ S ) that occurred as the protein was artificially denatured. DSC appeared more appropriate for the lubricants consisting of multiple protein types than the circular‐dichroism technique that has been used to investigate the unfolding of pure albumin solutions 25, 39. As this was a thermodynamically driven process, the values were given in Kelvin (K) rather than in degrees Celsius (°C).…”

Section: Methodsmentioning

confidence: 99%

Biochemical comparisons of osteoarthritic human synovial fluid with calf sera used in knee simulator wear testing

Brandt

Briere

Marr

et al. 2010

J Biomedical Materials Res

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Osteoarthritic human synovial fluid was obtained from the knees of 20 patients and was compared with four different calf sera solutions frequently used as lubricants in knee simulator wear testing. Assuming that the fluid after arthroplasty was the same as the fluid in patients with osteoarthritis, the total protein concentration, protein constituent fractions, osmolality, trace element concentrations, and the thermal stability obtained via differential scanning calorimetry were determined. Human synovial fluid, with an average total protein concentration of 34 g/L, was significantly different from all undiluted calf sera. However, alpha-calf serum and iron-supplemented alpha-calf serum were closest in protein constituent fractions (albumin, alpha-1-globulin, alpha-2-globulin, ss-globulin, and gamma-globulin) to human synovial fluid. Diluting calf sera with low-ion distilled water to a total protein concentration of 17 g/L (as recommended by ISO 14243) produced non-clinically relevant total protein concentration and osmolality levels. Performing the same dilution of iron-supplemented alpha-calf serum with phosphate-buffered saline solution and 1.5 g/L hyaluronic acid produced an artificial lubricant with both a clinically relevant level of osmolality and clinically relevant thermal stability as seen in human synovial fluid from patients with osteoarthritis. The present study suggested that alpha-calf serum, phosphate-buffered saline solution and hyaluronic acid were essential constituents of an artificial lubricant to mimic the major biochemical properties of human synovial fluid for simulator wear testing of total knee replacements.

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“…These protective layers keep the two surfaces from direct collision and thus decrease the possibility of wear of materials 9, 10. Some studies have attempted to investigate the lubrication ability of these biological molecules 11–13. It is still feasible to further improve the lubrication and reduce the wear of artificial joints in vivo .…”

Section: Introductionmentioning

confidence: 99%

Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer

Kumagai

Pham

Kataoka

et al. 2012

Intl Journal of Cancer

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This research aimed to validate the specificity of the newly developed nanobeacon for imaging the Thomsen-Friedenreich (TF) antigen, a potential biomarker of colorectal cancer. The imaging agent is comprised of a submicron-sized polystyrene nanosphere encapsulated with a Coumarin 6 dye. The surface of the nanosphere was modified with peanut agglutinin (PNA) and poly(N-vinylacetamide (PNVA) moieties. The former binds to Gal-β(1–3)GalNAc with high affinity while the latter enhances the specificity of PNA for the carbohydrates. The specificity of the nanobeacon was evaluated in human colorectal cancer cells and specimens, and the data was compared with immunohistochemical staining and flow cytometric analysis. Additionally, distribution of the nanobeacon in vivo was assessed using an “ intestinal loop” mouse model. Quantitative analysis of the data indicated that approximately 2 μg of PNA were detected for each mg of the nanobeacon. The nanobeacon specifically reported colorectal tumors by recognizing the tumor-specific antigen through the surface-immobilized PNA. Removal of TF from human colorectal cancer cells and tissues resulted in a loss of fluorescence signal, which suggests the specificity of the probe. Most importantly, the probe was not absorbed systematically in the large intestine upon topical application. As a result, no registered toxicity was associated with the probe. These data demonstrate the potential use of this novel nanobeacon for imaging the TF antigen as a biomarker for the early detection and prediction of the progression of colorectal cancer at the molecular level.

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Association of polyethylene friction and thermal unfolding of interfacial albumin molecules

Cited by 12 publications

References 38 publications

Investigation of protein content of synovial fluids with DSC in different arthritides

Investigation of protein content of synovial fluids with DSC in different arthritides

Biochemical comparisons of osteoarthritic human synovial fluid with calf sera used in knee simulator wear testing

Multifunctional nanobeacon for imaging Thomsen‐Friedenreich antigen‐associated colorectal cancer

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