Association of Polymorphisms in Candidate Genes with the Litter Size in Two Sheep Breeds

Yuan, Zehu; Zhang, Junxia; Li, Wanhong; Wang, Weimin; Li, Fadi; Yue, Xiangpeng

doi:10.3390/ani9110958

Cited by 24 publications

(21 citation statements)

References 45 publications

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“…The relevance of candidate genes on reproduction was determined by a literature search. Consequently, straightforward connections between LS and two candidate genes ( PPP1R13B and NGF ) have been reported [ 16 , 17 ]. Additionally, the potential reproductive roles of candidate genes in development of oocytes and sperm, zygotic transcriptional program, embryo implantation and development, and reproductive diseases were uncovered ( Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

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Litter Size of Sheep (Ovis aries): Inbreeding Depression and Homozygous Regions

Lin

Wang

et al. 2021

Genes

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Ovine litter size (LS) is an important trait showing variability within breeds. It remains largely unknown whether inbreeding depression on LS exists based on genomic homozygous regions, and whether the homozygous regions resulted from inbreeding are significantly associated with LS in sheep. We here reanalyze a set of single nucleotide polymorphism (SNP) chip of six breeds to characterize the patterns of runs of homozygosity (ROH), to evaluate inbreeding levels and inbreeding depressions on LS, and to identify candidate homozygous regions responsible for LS. Consequently, unique ROH patterns were observed among six sheep populations. Inbreeding depression on LS was only found in Hu sheep, where a significant reduction of 0.016, 0.02, and 0.02 per 1% elevated inbreeding FROH4–8, FROH>8 and the total inbreeding measure was observed, respectively. Nine significantly homozygous regions were found for LS in Hu sheep, where some promising genes for LS possibly via regulation of the development of oocytes (NGF, AKT1, and SYCP1), fertilization (SPAG17, MORC1, TDRD9, ZFYVE21, ADGRB3, and CKB), embryo implantation (PPP1R13B, INF2, and VANGL1) and development (DPPA2, DPPA4, CDCA4, CSDE1, and ADSSL1), and reproductive health (NRG3, BAG5, CKB, and XRCC3) were identified. These results from the present study would provide insights into the genetic management and complementary understandings of LS in sheep.

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Section: Resultsmentioning

confidence: 99%

“…In pigs, 17β-estradiol, one important reproductive hormone, was found to promote transcription of p53 [ 24 ]. Interestingly, the polymorphism of NGF , playing a role in augmenting folliculogenesis, has been reported to be associated with LS in both sheep and goats [ 17 , 25 , 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Litter Size of Sheep (Ovis aries): Inbreeding Depression and Homozygous Regions

Lin

Wang

et al. 2021

Genes

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“…However, growth traits of Shaanbei White Cashmere goats need improving. Rapid, accurate, as well as an effective method of MAS is presented in [42][43][44]. Herein, this study aimed at finding an effective and faithful DNA maker which could accelerate the process of selective breeding in goat rearing.…”

Section: Introductionmentioning

confidence: 99%

Myostatin (MSTN) Gene Indel Variation and Its Associations with Body Traits in Shaanbei White Cashmere Goat

Feng

Wang

et al. 2020

Animals

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Myostatin (MSTN) gene, also known as growth differentiation factor 8 (GDF8), is a member of the transforming growth factor-beta super-family and plays a negative role in muscle development. It acts as key points during pre- and post-natal life of amniotes that ultimately determine the overall muscle mass of animals. There are several studies that concentrate on the effect of a 5 bp insertion/deletion (indel) within the 5’ untranslated region (5’ UTR) of goat MSTN gene in goats. However, almost all sample sizes were below 150 individuals. Only in Boer goats, the sample sizes reached 482. Hence, whether the 5 bp indel was still associated with the growth traits of goats in large sample sizes which were more reliable is not clear. To find an effective and dependable DNA marker for goat rearing, we first enlarged the sample sizes (n = 1074, Shaanbei White Cashmere goat) which would enhance the robustness of the analysis and did the association analyses between the 5 bp indel and growth traits. Results uncovered that the 5 bp indel was significantly related to body height, height at hip cross, and chest width index (p < 0.05). In addition, individuals with DD genotype had a superior growing performance than those with the ID genotype. These findings suggested that the 5 bp indel in MSTN gene are significantly associated with growth traits and the specific genotype might be promising for maker-assisted selection (MAS) of goats.

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“…In our experiment, similar results were obtained; supplementation of the ewe’s diet with FA during pregnancy markedly improved muscle layer thickness in offspring, suggesting an improvement in intestinal function. Furthermore, it is generally accepted that litter size is also an important factor affecting the development of the offspring [ 34 ]. Depending on the number of fetuses present in the uterus, the same maternal nutritional level provides different amount of nutrients for tissue growth, which can lead to obvious discrepancy in fetal development of different litter sizes [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Maternal Folic Acid Supplementation Differently Affects the Small Intestinal Phenotype and Gene Expression of Newborn Lambs from Differing Litter Sizes

Li¹,

Wang²,

Li³

et al. 2020

Animals

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The purpose of this study was to investigate the effect of maternal dietary folic acid (FA) supplementation during gestation on small intestinal development of newborn lambs of different litter sizes, focusing on the intestinal morphology and development-, apoptosis- and digestion-related genes expression. One hundred and twenty Hu ewes (Ovis aries) were inseminated and randomly allotted to three groups. One group received a control diet [without FA supplementation, control (CON)] and the other two groups received control diets supplemented with different amount of FA [16 or 32 mg FA per kg dry matter (DM), i.e., F16 and F32] during pregnancy. After lambing, according to the dietary FA levels and litter size (twins, TW; triplets, TR), the neonatal lambs were divided into 6 (TW-CON, TW-F16, TW-F32, TR-CON, TR-F16, TR-F32) treatment groups. The results showed that the ratio of small intestinal weight to live body weight and the thickness of the intestinal muscle layer in the offspring was enhanced significantly with increasing maternal FA supplementation (p < 0.05). Meanwhile, the expression levels of insulin-like growth factor I (IGF-I), B-cell lymphoma-2 (BCL-2) and sodium/glucose co-transporter-1 (SGLT1) in the small intestines of the newborn lambs were increased, while the opposite was true for Bcl2-associated × (BAX) in response to FA supplementation (p < 0.05). Moreover, the small intestinal weights of twins were significantly higher than those of triplets (p < 0.01), and the expression levels of IGF-I (p < 0.05), sucrase-isomaltase (SI) (p < 0.05) and solute carrier family 2 member 5 (SLC2A5) (p < 0.01) were significantly lower than those in triplets. These findings suggest that maternal FA supplementation could improve the offspring’s small intestinal phenotype and the expression of development-, apoptosis- and digestion-related genes, so it could promote the small intestinal development of newborn lambs. Furthermore, the small intestine phenotypic development of twins was generally better than that of triplets, while the expression levels of the above genes of twins were lower than those of triplets.

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Association of Polymorphisms in Candidate Genes with the Litter Size in Two Sheep Breeds

Cited by 24 publications

References 45 publications

Litter Size of Sheep (Ovis aries): Inbreeding Depression and Homozygous Regions

Litter Size of Sheep (Ovis aries): Inbreeding Depression and Homozygous Regions

Myostatin (MSTN) Gene Indel Variation and Its Associations with Body Traits in Shaanbei White Cashmere Goat

Maternal Folic Acid Supplementation Differently Affects the Small Intestinal Phenotype and Gene Expression of Newborn Lambs from Differing Litter Sizes

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