Association of polymorphisms of renin angiotensin system and endothelial nitric oxide synthase genes with premature cardiovascular disease in an Iranian population

Abstract

Introduction: The study on polymorphisms and their relationship with diseases is very important for risk assessment. The aim of this study was to determine the relationship between early risk of coronary artery disease(CAD) with renin angiotensin(RAS) genes and endothelial nitric oxide synthase(eNOS) gene in a sample of the Iranian population. Methods & Materials: In this cross-sectional study, 63 patients with premature CAD and 72 healthy samples were enrolled. Polymorphism of the promotor region of eNOS- and ACE-I/D(Angiotensin Converting Enzyme-I/D) polymorphism were evaluated. Polymerase chain reaction(PCR) test and PCR-RFLP(Restriction Fragment Length Polymorphism) was performed for ACE and eNOS-786 gene, respectively. Results: The frequency of deletion(D) for ACE gene was 96% for patients and 61% for healthy controls and was significantly higher in patients(P<0.001). Conversely, the number of defective C allele for eNOS gene was similar in both groups(p>0.9). Conclusion: ACE polymorphism seems to be an independent risk factor for premature CAD.

Association of rs5051 and rs699 polymorphisms in angiotensinogen with coronary artery disease in Iranian population: A case-control study

“…As a multifactorial disease, CAD is one of the most critical concerns regarding population health worldwide and is associated with multiple environmental and genetic factors. [23] Previously, heterogeneity in CAD severity in the Iranian population was associated with an increased risk in Gilak and Turks. [24,25] Here, we compared the frequency of CAD in the Iranian North population, which includes three major groups: Fars, Turkmen, and Sistani.…”