Association of porcine UCP3 gene polymorphisms with fatness traits in a PietrainJinhua F2 population

Abstract

Uncoupling protein 3 (UCP3) is a mitochondrial transmembrane carrier which uncouples oxidative phosphorylation and plays an important role in energy homeostasis and fatty acid transporting. In this study, two missense mutations (T221C and A448G) were detected in porcine UCP3 gene. Genotype frequencies were analyzed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) in eight pig breeds (n = 384). The results revealed that Chinese native breeds had fixed genotype CC at T221C polymorphic loci. Allele G at the A448G loci has higher frequency than allele A in all pure breeds, except for Pietrain. Association analysis between variants and fatness traits was carried out in a Pietrain×Jinhua F2 population (n = 274). The results showed that two single nucleotide polymorphism (SNPs) were significantly associated with intramuscular fat content (p = 0.023 and p = 0.000, respectively). These results indicated that single nucleotide polymorphism of UCP3 gene is potentially associated with fatness traits in pig.

Genome-Wide Expression Profiling of mRNAs, lncRNAs and circRNAs in Skeletal Muscle of Two Different Pig Breeds

“…Overexpression of UCP3 can increase fatty acid oxidation in transgenic mouse skeletal muscle [ 21 ]. In a previous study, UCP3 gene polymorphisms were significantly associated with IMF content [ 63 ]. The UCP3 gene is more highly expressed in the skeletal muscle of obese pigs compared to lean pigs [ 8 , 64 ].…”