Association of proinflammatory cytokine IL-20 gene polymorphism with psoriasis in north Indian population

Wani, Aadil; Ganai, Bashir Ahmad; Akhtar, Tahseena; Narang, Tarun; Kaur, Rajinder

doi:10.1016/j.ejmhg.2017.09.002

Cited by 6 publications

(7 citation statements)

References 23 publications

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“…In the north Indian population, IL-19 –35402 C/C (rs2243158) genotype was associated with an increased risk of psoriasis. Researchers did not find any significant association of IL-19 –42232 C/A (rs2243188), –38386 T/C (rs2073186) with psoriasis [ 61 ]. In turn, the study on the European population reveals that there is no significant difference of IL-19 gene SNPs in psoriasis patients and controls [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Combination of FLG mutations and SNPs of IL-17A and IL-19 influence on atopic dermatitis occurrence

Klonowska¹,

Gleń²,

Nowicki³

et al. 2022

pdia

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Introduction Atopic dermatitis (AD) is a heterogeneous inflammatory skin disease. A fresh look on the AD pathophysiology has focused on the skin barrier defect and immune dysfunctions. IL-17A and IL-19 seem to play role in AD pathogenesis. Aim The aim was to investigate associations of SNPs of IL-17A (rs2275913) and IL-19 (rs22431188) with AD features, course and occurrence. Searching for prognostic panels composed of FLG (2282del4, R501X) mutations with IL-17A and IL-19 polymorphisms. Material and methods Blood samples were collected from 239 patients with AD and 170 controls. Two SNPs, IL-17A and IL-19 and FLG null mutations were analyzed. PCR and RFLP restriction fragment length polymorphism analysis were used. SCORAD score to establish AD severity, VAS to estimate pruritus. Results None polymorphisms of studied cytokines caused more frequent AD occurrence compared to controls. We found no associations between IL-17A and IL-19 gene polymorphisms and AD severity (respectively p = 0.954; p = 0.498), IgE level ( p = 0.707; p = 0.584), VAS ( p = 0.953; p = 0.478), concomitant asthma ( p = 0.488, p = 0.764). The G/G genotype in IL-17A (rs2275913) occurrence with coexisting 2282del4 FLG gene mutation increased the AD frequency 9 times ( p = 0.0266). Conclusions The SNPs of IL-17A rs2275913 and IL-19 rs22431188 SNP seem not to have influence on AD course and occurrence while studied alone. The coexistence of GG genotype of IL-17A and 2282del4 FLG mutation may play a role as prognostic AD factor.

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Section: Discussionmentioning

confidence: 99%

Combination of FLG mutations and SNPs of IL-17A and IL-19 influence on atopic dermatitis occurrence

Klonowska¹,

Gleń²,

Nowicki³

et al. 2022

pdia

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“…Set of four primers, specific to each SNP, were designed by Web based allele specific primer designing tool (WASP) and Primer 1, and synthesized from G. Biosciences (Noida, India). 15,16 Each PCR reaction was carried out in a total volume of 10 μl, containing 100 ng of template DNA, 10 pmol of each inner primer, 20 pmol of each outer primer and optimized concentration of commercially available mastermix (Sigma, G. Biosciences). The amplified products were separated by electrophoresis on a 2% agarose gel stained with ethidium bromide and visualized using Gel documentation system.…”

Section: Genotypic Analysismentioning

confidence: 99%

Role of proinflammatory cytokines TNF-α (rs1800629) and IL-6 (rs1800795) in the pathogenesis of polycystic ovarian syndrome in North Indian females

Kaur,

Kaur

2024

Int J Reprod Contracept Obstet Gynecol

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Background: This study aimed to determine whether TNF-α and IL-6 gene polymorphism, their epistatic effects and haplotypes confer pathogenesis of PCOS in north Indian females. Methods: A case-control study comprising DNA samples of 401 females (200 PCOS cases and 201 controls) of reproductive age. All the subjects were genotyped for TNF-α (-308 G/A) and IL-6 (-174 G/C) genes by tetra-primer ARMS PCR. Results: There were 41.5% PCOS females revealing hirsutism, 45.5% acne and 36% alopecia. High BMI (p=0.008) and W/H ratio (p=0.0001) was observed among PCOS cases. Frequency of minor allele A for -308 G/A TNF-α was significantly higher in PCOS cases than controls indicating 1.4 fold increased risk for PCOS (p=0.05, OR=1.41, 95% CI=1.00-1.99). -174 G/C IL-6 gene was in association with the decreased risk of PCOS. The epistatic effects of all the possible combinations of both the SNPs shows statistically significant differences (Interaction p value=0.014) indicating modulating effects of TNF-α and IL-6 polymorphism in response to PCOS. Haplotype HT3 AG (p=0.003, OR=2.22, 95% CI=1.31-3.78) was associated with increased risk of PCOS. Conclusions: The present findings suggest that TNF-α and IL-6 might contribute to pathogenesis of PCOS in north Indian females irrespective of a polymorphism of TNF-α (-308 G/A) and IL-6 (-174 G/C) genes.

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“…Several psoriasis susceptibility loci have been associated with cytokine signaling pathways such as IL-20, IL-17/23 and NF-κB signaling pathways. Recent studies reported IL-20 HT GGA haplotype as the susceptibility loci for both psoriasis and psoriatic arthritis in North Indian population, where patients with this haplotype had increased IL-20 levels (Lebre et al, 2012; Wani et al, 2018). The IL-12B risk haplotype (A allele of rs3212227 and G allele of rs6887695) has been identified in European, Danish, and Asian populations, specifically the Thai and Japanese populations (Johnston et al, 2013; Zhu et al, 2013;Guo et al, 2014; Tamari et al, 2014; Loft et al, 2018).…”

Section: Genetic Variants and Their Association With Immuno-pathologimentioning

confidence: 99%

Combining Understanding of Immunological Mechanisms and Genetic Variants Toward Development of Personalized Medicine for Psoriasis Patients

et al. 2019

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Psoriasis is multifactorial disease with complex genetic predisposition. Recent advances in genetics and genomics analyses have provided many insights into the relationship between specific genetic predisposition and the immunopathological mechanisms driving psoriasis manifestation. Novel approaches which utilize array-based genotyping technologies such as genome-wide association studies and bioinformatics tools for transcriptomics analysis have identified single nucleotide polymorphisms, genes and pathways that are associated with psoriasis. The discovery of these psoriasis-associated susceptibility loci, autoimmune targets and altered signaling pathways have provided opportunities to bridge the gap of knowledge from sequence to consequence, allowing new therapeutic strategies for the treatment of psoriasis to be developed. Here, we discuss recent advances in the field by highlighting how immune functions associated with psoriasis susceptibility loci may contribute to disease pathogenesis in different populations. Understanding the genetic variations in psoriasis and how these may influence the immunological pathways to cause disease will contribute to the efforts in developing novel and targeted personalized therapies for psoriasis patients.

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Association of proinflammatory cytokine IL-20 gene polymorphism with psoriasis in north Indian population

Cited by 6 publications

References 23 publications

Combination of FLG mutations and SNPs of IL-17A and IL-19 influence on atopic dermatitis occurrence

Combination of FLG mutations and SNPs of IL-17A and IL-19 influence on atopic dermatitis occurrence

Role of proinflammatory cytokines TNF-α (rs1800629) and IL-6 (rs1800795) in the pathogenesis of polycystic ovarian syndrome in North Indian females

Combining Understanding of Immunological Mechanisms and Genetic Variants Toward Development of Personalized Medicine for Psoriasis Patients

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