Association of Rous sarcoma virus DNA with Xenopus laevis spermatozoa and its transfer to ova through fertilization

Habrová, V.; Takác, M; Navrátil, J.; Mácha, J; Čéšková, N.; Jonák, Jiřı́

doi:10.1002/(sici)1098-2795(199607)44:3<332::aid-mrd7>3.3.co;2-w

Cited by 11 publications

(25 citation statements)

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“…Several authors have shown that after the in vitro incubation of the male gametes with foreign genes, spermatozoa can generate TA. The production of trans- genic embryos or adults using this procedure has been shown in different animal species as fishes (Khoo et al, 1992;Symonds et al, 1994), amphibians (Habrovà et al, 1996 ), birds (Rottman et al, 1992;Nakanishi and Iritani, 1993), and mammals (Lavitrano et al, 1989;Gagné et al, 1991;Schellander et al, 1995;Sperandio et al, 1996). Recently, it has been shown that membrane-disrupted spermatozoa coinjected with exogenous genes (GFP or ␤-galacosidase gene constructions) into the egg cytoplasm generated 64% to 94% transgeneexpressing embryos and an offspring in which about 20% of pups express the integrated transgene (Perry et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Generation of genetically modified mice by spermatozoa transfection in vivo: Preliminary results

Huguet¹,

Esponda²

2000

Mol. Reprod. Dev.

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Section: Discussionmentioning

confidence: 99%

Generation of genetically modified mice by spermatozoa transfection in vivo: Preliminary results

Huguet¹,

Esponda²

2000

Mol. Reprod. Dev.

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“…DNA from Rous sarcoma virus binds to Xenopus laevis spermatozoa and is transferred to ova during fertilization, inducing developmental malformations in 25 to 30% of embryos (134). In gibbons, sperm abnormalities are frequently encountered and have often been shown to be related to the presence of herpesvirus (36,327).…”

Section: Animal Semenmentioning

confidence: 97%

Viruses in the Mammalian Male Genital Tract and Their Effects on the Reproductive System

Dejucq

Jégou

2001

Microbiol Mol Biol Rev

290

220

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This review describes the various viruses identified in the semen and reproductive tracts of mammals (including humans), their distribution in tissues and fluids, their possible cell targets, and the functional consequences of their infectivity on the reproductive and endocrine systems. The consequences of these viral infections on the reproductive tract and semen can be extremely serious in terms of organ integrity, development of pathological and cancerous processes, and transmission of diseases. Furthermore, of essential importance is the fact that viral infection of the testicular cells may result not only in changes in testicular function, a serious risk for the fertility and general health of the individual (such as a fall in testosteronemia leading to cachexia), but also in the possible transmission of virus-induced mutations to subsequent generations. In addition to providing an exhaustive account of the data available in these domains, this review focuses attention on the fact that the interface between endocrinology and virology has so far been poorly explored, particularly when major health, social and economical problems are posed. Our conclusions highlight the research strategies that need to be developed. Progress in all these domains is essential for the development of new treatment strategies to eradicate viruses and to correct the virus-induced dysfunction of the endocrine system

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“…However, additional strategies have been developed. Reports have highlighted the use of transfected spermatozoa from different species to generate transgenic animals, including Xenopus (Habrova et al, 1996; Takac et al, 1998; Smith and Spadafora, 2005). Different strategies have been employed to transform sperm DNA, such as liposome transfection, electroporation and dipping cells in a solution of naked DNA.…”

Section: Smgt (Sperm‐mediated Gene Transfer)mentioning

confidence: 99%

Transgenesis procedures in Xenopus

Chesneau

Sachs

Chai

et al. 2008

Biology of the Cell

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Stable integration of foreign DNA into the frog genome has been the purpose of several studies aimed at generating transgenic animals or producing mutations of endogenous genes. Inserting DNA into a host genome can be achieved in a number of ways. In Xenopus, different strategies have been developed which exhibit specific molecular and technical features. Although several of these technologies were also applied in various model organizms, the attributes of each method have rarely been experimentally compared. Investigators are thus confronted with a difficult choice to discriminate which method would be best suited for their applications. To gain better understanding, a transgenesis workshop was organized by the X-omics consortium. Three procedures were assessed side-by-side, and the results obtained are used to illustrate this review. In addition, a number of reagents and tools have been set up for the purpose of gene expression and functional gene analyses. This not only improves the status of Xenopus as a powerful model for developmental studies, but also renders it suitable for sophisticated genetic approaches. Twenty years after the first reported transgenic Xenopus, we review the state of the art of transgenic research, focusing on the new perspectives in performing genetic studies in this species.

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Association of Rous sarcoma virus DNA with Xenopus laevis spermatozoa and its transfer to ova through fertilization

Cited by 11 publications

References 0 publications

Generation of genetically modified mice by spermatozoa transfection in vivo: Preliminary results

Generation of genetically modified mice by spermatozoa transfection in vivo: Preliminary results

Viruses in the Mammalian Male Genital Tract and Their Effects on the Reproductive System

Transgenesis procedures in Xenopus

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