Association of GPIa and COX‐2 gene polymorphism with aspirin resistance

Abstract

ObjectiveThis study aimed to explore the association between GPIa, COX‐2 gene polymorphisms and aspirin resistance in the ischemic stroke patients from the southern part of Jiangsu province.MethodsIn all, 97 patients with acute ischemic stroke were enrolled in the study. GPIa gene polymorphism at 807C>T (rsl126643) locus and COX‐2 gene polymorphism at ‐765G>C (rs20417) locus were genotyped by PCR pyrosequencing technology. Patients were divided into the aspirin sensitivity (AS) group and aspirin resistance (AR) group according to the platelet aggregation rate. The relationship between the two gene polymorphisms and aspirin resistance was investigated and analyzed.ResultsThe distribution of the genotype (CC, CT, TT, CT + TT, and CC) and the frequency of allele T of GPIa gene at 807C>T locus were significantly different in AS and AR groups in female patients (P < .05). Logistic regression analysis showed that the genotype of CT+TT at 807C>T locus was significantly correlated with AR after adjustment for relative factors (P = .047, OR = 4.856, 95% CI: 1.020–23.108). There were no significant differences in the genotype distribution and allele frequency of the COX‐2 gene ‐765G>C site between two groups (P > .05).Conclusion GPIa gene polymorphism at 807C>T locus was associated with AR in Chinese Han females, and the expression of allele T increased the incidence of AR. The gene polymorphism of COX‐2 gene at ‐765G>C locus was not significantly correlated with AR.

Genetic Association Study and Machine Learning to Investigate Differences in Platelet Reactivity in Patients with Acute Ischemic Stroke Treated with Aspirin

“…The second limitation is related to the size of the studied population. It seems to be large enough compared with other studies in the field [ 21 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. However, clarifying the genetic background of aspirin treatment failure, which is affected by numerous clinical parameters and studied SNPs, requires further studies including larger and more clinically uniform cohorts of patients.…”

“…The SNPs were selected based on the literature data. All of them are involved in platelet activation and aggregation, and their contribution to aspirin resistance is discussed in numerous studies [ 6 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. We evaluated the distribution of 16 genetic markers in the AS and AR groups in a cohort of 461 patients with acute ischemic stroke.…”

“…The genes involved in the COX-independent platelet activation pathways as well as platelet glycoprotein genes might also be involved in AR. The effect of polymorphisms in the genes HMOX1 [ 24 ], PLA2G7 [ 30 ], ADRA2A [ 30 ], ITGB3 [ 22 , 32 ], GPIBA [ 33 ], ITGA2 [ 34 ] and PEAR1 [ 35 , 36 , 37 ] on inter-individual variations in the aspirin response has been discussed. A locus on chromosome 9p21.3 , associated with CVD and ischemic stroke, was also connected with AR [ 30 , 38 ].…”

Genetic Polymorphism of ITGA2 C807T Collagen Receptor Encoding Gene of Aspirin Therapy among Javanese-Indonesian Healthy Respondents

“…Numerous studies focusing on the polymorphism of ITGA2 C807T gene that encodes the GPIa collagen receptor have proved its correlation with aspirin resistance. A study of Han Chinese patients with acute ischemic stroke shows that the T allele significantly correlates with aspirin resistance (OR 4.86) [7]. Similarly, a metaanalysis reveals that rs1126643 (C807T) polymorphism becomes the genetic variation associated with aspirin insensitivity [8], risk of ischemic stroke among the Asian populations [9], and risk of elevated levels of serum cholesterol [10].…”

Ultrasensitive electrocatalytic detection of COX-2 rs20417: relying on 3D interconnected architecture of Pt-LSSUs@PAA nanostructures for sensor interface modification

“…Despite adequate aspirin therapy, 6.2-57% patients still experience cardiovascular events, the phenomenon named as aspirin resistance (AR) [2]. Researchers have reported that genetic variability in cyclooxygenase-2 (COX-2)-765G/C (rs20417) affect aspirin response with cardiovascular disease and could be used as specific genetic marker of AR [3,4]. Therefore, it is crucial to prescreen patients for their genotypes before prescribing aspirin to facilitate target therapy and decreasing the side effects associated with prolonged administration.…”