Association of selected SNP with carcass and taste panel assessed meat quality traits in a commercial population of Aberdeen Angus-sired beef cattle

Abstract

BackgroundThe purpose of this study was to evaluate the effects of eight single nucleotide polymorphisms (SNP), previously associated with meat and milk quality traits in cattle, in a population of 443 commercial Aberdeen Angus-cross beef cattle. The eight SNP, which were located within five genes: μ-calpain (CAPN1), calpastatin (CAST), leptin (LEP), growth hormone receptor (GHR) and acylCoA:diacylglycerol acyltransferase 1 (DGAT1), are included in various commercial tests for tenderness, fatness, carcass composition and milk yield/quality.MethodsA total of 27 traits were examined, 19 relating to carcass quality, such as carcass weight and fatness, one mechanical measure of tenderness, and the remaining seven were sensory traits, such as flavour and tenderness, assessed by a taste panel.ResultsAn SNP in the CAPN1 gene, CAPN316, was significantly associated with tenderness measured by both the tenderometer and the taste panel as well as the weight of the hindquarter, where animals inheriting the CC genotype had more tender meat and heavier hindquarters. An SNP in the leptin gene, UASMS2, significantly affected overall liking, where animals with the TT genotype were assigned higher scores by the panellists. The SNP in the GHR gene was significantly associated with odour, where animals inheriting the AA genotype produced steaks with an intense odour when compared with the other genotypes. Finally, the SNP in the DGAT1 gene was associated with sirloin weight after maturation and fat depth surrounding the sirloin, with animals inheriting the AA genotype having heavier sirloins and more fat.ConclusionThe results of this study confirm some previously documented associations. Furthermore, novel associations have been identified which, following validation in other populations, could be incorporated into breeding programmes to improve meat quality.

Boz Irk Sığırın Et Gevrekliğini Belirlemede CAST ve CAPN1 Genlerindeki Kimi SNP’lerin Kullanımı

“…The frequency ratio of the TGC with the CAPN1 316 CC genotype is quite low. Except for Aberdeen Angus [22] and Hanwoo cattle [24] , Bos taurus with CC genoype have not been observed in previous studies [11,25,26] . The CAPN1 4751 C allele frequency (0.412) that is considered to have a favourable impact on the beef tenderness seems to be higher than the other breeds except for Aberdeen Angus (0.65) [22] and Hanwoo cattle (0.80) [24] .…”

“…Except for Aberdeen Angus [22] and Hanwoo cattle [24] , Bos taurus with CC genoype have not been observed in previous studies [11,25,26] . The CAPN1 4751 C allele frequency (0.412) that is considered to have a favourable impact on the beef tenderness seems to be higher than the other breeds except for Aberdeen Angus (0.65) [22] and Hanwoo cattle (0.80) [24] . TGC with the CAPN1 4751 CC genotype frequency (0.171) were found to be higher compared to Nellore (0.00) and Canchim (0.07) [4] , Simmental and their crossbreeds (0.05) [26] .…”

“…TGC with the CAPN1 4751 CC genotype frequency (0.171) were found to be higher compared to Nellore (0.00) and Canchim (0.07) [4] , Simmental and their crossbreeds (0.05) [26] . The ratio of TGC with CC genotype, which is favourable for meat tenderness, is lower than Brahman crossbreeds (0.53) [26] , Aberdeen Angus (0.41) [22] and Hanwoo cattle (0.64) [24] populations.…”

“…Previous researchers reported that less force was applied to the LD muscle of the cattle with the UoG-CAST CC genotype than the cattle with CG and GG genotypes and those cattle with CC genotype have more tender meat [5,6,11,22] . SF was applied to LD of Aberdeen Angus, Limousine, Charolais, Simmental and their crossbreeds on the 21 st day after death as avarage 4.53±0.12 kg/ cm 2 for with the UoG-CAST CC and 4.91±0.15 kg/cm 2 for with GG genotype, and the difference was statistically significant (P<0.05) [5] .…”

“…The C allele frequency in our sample which is made up of purebred TGC is 0.559, which is also lower than the frequencies reported by previous researchers [20,21] . For the UoG-CAST SNP genotypes Gill et al [22] found the CC, CG and GG genotypic frequencies in Aberdeen Angus cattle as 0.41, 0.47 and 0.12, respectively. On the other hand, Schenkel et al [5] found them as 0.430, 0.398 and 0.172, respectively for Bos taurus while Curi et al [11] found 0.377, 0.491 and 0.132, respectively for purebred Bos indicus Nellores.…”