Association of serine racemase gene polymorphism with type 2 diabetes mellitus

Abstract

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance and β-cell dysfunction, with a significant global impact. Genome-wide association studies (GWAS) have identified several genetic polymorphisms linked to T2DM, including the rs391300 polymorphism in the SRR gene. This study aimed to evaluate the association between the rs391300 polymorphism and T2DM in the Saudi population. A total of 160 participants, comprising 80 T2DM patients and 80 healthy controls, were genotyped using quantitative PCR with VIC and FAM probes. The results revealed a significant association between T2DM and age, body mass index (BMI), glucose levels, and cholesterol levels. Genotype and allele frequency analysis demonstrated that the rs391300 polymorphism was linked to a higher risk of T2DM (GA vs. AA: OR = 4.75, 95% CI: 1.52–14.94, p = 0.04; A vs. G: OR = 4.33, 95% CI: 1.42–13.27, p = 0.005). Additionally, ANOVA analysis indicated a significant association with weight and BMI (p = 0.01). This study provides evidence of a positive association between the rs391300 polymorphism in the SRR gene and T2DM in the Saudi population.