Association of several small heat-shock proteins with reproductive tissues in the nematode Caenorhabditis elegans

Ding, Lillian; Candido, E. P. M.

doi:10.1042/bj3510013

Cited by 14 publications

(11 citation statements)

References 9 publications

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“…This response also mirrors and exaggerates the response induced by temperature stress alone (Rutherford and Lindquist 1998). The study by Ding and Candido (2000) may thus hint at a molecular mechanism behind the observed temperature-sensitivity of spermathecal development. Waddington's (1953) classical demonstration of temperaturedependent genetic assimilation in D. melanogaster highlighted the importance of cryptic genetic variation for major evolutionary transitions.…”

Section: Numbersupporting

confidence: 52%

Section: Discussionmentioning

confidence: 92%

“…We have similarly recorded a few individuals with five, six, and eight spermathecae, respectively when raising yellow dung flies under warm laboratory conditions. Immunohistochemical data on the roundworm Caenorhabditis elegans show that heat shock proteins (Hsp) are overrepresented and over-expressed in the spermathecal tissue during development (Ding and Candido 2000). The role of Hsp in capacitating phenotypic diversity through buffering against environmental stress has been demonstrated in diverse taxa such as insects, flowering plants, and yeast (reviewed in: Sangster et al 2004).…”

Section: Numbermentioning

confidence: 99%

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High Temperatures Reveal Cryptic Genetic Variation in a Polymorphic Female Sperm Storage Organ

et al. 2011

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Section: Numbersupporting

confidence: 52%

Section: Discussionmentioning

confidence: 92%

Section: Numbermentioning

confidence: 99%

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High Temperatures Reveal Cryptic Genetic Variation in a Polymorphic Female Sperm Storage Organ

et al. 2011

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“…When considered together with the phenotypes displayed by daf-21 (p673), these findings led us to conclude that the ubiquitous distribution of DAF-21 seems to be critical for viability of the embryo as well as of early larval stages; furthermore, DAF-21 expression in late larval stages or in the adult, when daf-21 mRNA expression is limited to germline cells, seems to have more significance in germline development. In a situation similar to that for daf-21, several of the heat shock protein family members with small molecular weights, the small heat shock proteins (smHSP), including hsp12, hsp25, and hsp43, show an association with reproductive tissues (Ding & Candido 2000).…”

Section: Discussionmentioning

confidence: 94%

Caenorhabditis elegans DAF‐21 (HSP90) is characteristically and predominantly expressed in germline cells: Spatial and temporal analysis

Inoue

Takamura

Yamae³

et al. 2003

Dev Growth Differ

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Three monoclonal antibodies against antigens that exist in the Caenorhabditis elegans germ line have previously been described. In the present study, a full-length mRNA for one of these antigens was isolated, and by sequencing its corresponding cDNA, it was predicted that the protein would show a high homology with the 90 kDa heat shock protein (HSP90) in other species, and with the protein of daf-21 , a previously identified hsp90 homologue. The spatial and temporal distribution of the antigen (DAF-21) was analyzed in C. elegans , and the localization of daf-21 mRNA, as detected by in situ hybridization, agreed with that detected by the monoclonal antibody. Under normal conditions, daf-21 mRNA is characteristically distributed in postembryonic germ cells derived from Z2 and Z3 cells in both hermaphrodites and males. Under heat stress conditions, however, daf-21 mRNA was not only detected in germ cells, but also apparently expressed all over the body. In addition, the DAF-21 protein seemed to be localized in the perinuclear region of somatic cells.

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“…To follow KGB-1-dependent gene expression outside of the intestine, we used hsp-43, a KGB-1 target that was previously described to localize to cell-cell junctions, in particular interfaces between epidermis and muscle cells, and is thought to be expressed in the epidermis (Ding and Candido 2000;Zhang et al 2017). Unlike the intestinal genes described above, only epidermal activation of KGB-1 could induce larval hsp-43 gene expression ( Figure 5G).…”

Section: Cell-autonomous and -Nonautonomous Contributions Of Kgb-1 Tomentioning

confidence: 99%

Integration of Stress Signaling in Caenorhabditis elegans Through Cell-Nonautonomous Contributions of the JNK Homolog KGB-1

2018

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Dealing with physiological stress is a necessity for all organisms, and the pathways charged with this task are highly conserved in Metazoa . Accumulating evidence highlights cell-nonautonomous activation as an important mode of integrating stress responses at the organism level. Work in Caenorhabditis elegans highlighted the importance of such regulation for the unfolded protein response (UPR) and for gene expression downstream of the longevity-associated transcription factor DAF-16. Here we describe a role for the JNK homolog KGB-1 in cell-nonautonomous regulation of these two response modules. KGB-1 protects developing larvae from heavy metals and from protein folding stress (which we found to be independent of canonical UPR pathways), but sensitizes adults to the same stress, further shortening life span under normal conditions. This switch is associated with age-dependent antagonistic regulation of DAF-16. Using transgenic tissue-specific KGB-1 expression or tissue-specific KGB-1 activation we examined the contributions of KGB-1 to gene regulation, stress resistance, and life span. While cell-autonomous contributions were observed, particularly in the epidermis, cell-nonautonomous contributions of neuronal KGB-1 (and also in muscle) were effective in driving intestinal gene induction, age-dependent regulation of intestinal DAF-16, and stress resistance, and did not require KGB-1 expression in the target tissue. Additional genetic analyses revealed requirement for UNC-13 in mediating neuronal contributions, indicating involvement of neurotransmission. Our results expand the role of KGB-1 in stress responses from providing local cellular protection to integrating stress responses at the level of the whole organism.

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Association of several small heat-shock proteins with reproductive tissues in the nematode Caenorhabditis elegans

Cited by 14 publications

References 9 publications

High Temperatures Reveal Cryptic Genetic Variation in a Polymorphic Female Sperm Storage Organ

High Temperatures Reveal Cryptic Genetic Variation in a Polymorphic Female Sperm Storage Organ

Caenorhabditis elegans DAF‐21 (HSP90) is characteristically and predominantly expressed in germline cells: Spatial and temporal analysis

Integration of Stress Signaling in Caenorhabditis elegans Through Cell-Nonautonomous Contributions of the JNK Homolog KGB-1

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