Association of SNP and STR polymorphisms of insulin-like growth factor 2 receptor (IGF2R) gene with milk traits in Holstein-Friesian cows

Abstract

The objective of the study reported in this Research Communication was to investigate the association of polymorphisms in the insulin-like growth factor receptor 2 (IGF2R) gene with milk traits in 283 Polish Holstein-Friesian (PHF) cows from the IGAB PAS farm in Jastrzębiec. IGF2R regulates the availability of biologically active IGF2 which is considered as a genetic marker for milk or meat production in farm animals. Two novel genetic polymorphisms were identified in the bovine IGF2R gene: a polymorphic TG-repeat in intron 23 (g.72389 (TG)15-67), and a g.72479 G > A SNP RFLP-StyI in exon 24. The following milk traits were investigated: milk yield, protein and fat yield, SCC and lactose content. To determine the influence of the IGF2R STR and SNP genotypes on the milk traits, we used the AI-REML (average information restricted maximum likelihood) method with repeatability, multi-trait animal model based on test-day information using DMU package. Statistical analysis revealed that the G/A genotype (P ≤ 0·01) was associated with milk and protein yield, lactose content and somatic cell count (SCC) in Polish HF cows. TGn (29/22, 28/29, 28/22, 28/28) genotypes were associated with high values for milk, (28/22, 28/23) with protein and fat yield, (25/20) with lactose content, and (29/33, 28/28) with low SCC. We suggest that the IGF2R gene polymorphisms could be useful genetic markers for dairy production traits in cattle.

Bioactive peptides from milk: animal determinants and their implications in human health

“…With the recent development of analytical tools and genomic resources, it is possible to identify Quantitative Trait Loci (QTL) and thus, study the influence of multiple genes on biological traits and milk composition (Dux et al ., 2018). Thus, QTL could contribute to find genes that can be used in breeding programs via marker-assisted selection.…”

A novel (ATC)n microsatellite locus is associated with litter size in an indigenous Chinese pig

“…and LEP genes in cattle may influence the milk traits, feed intake, milk fatty acid composition and metabolic state (Avondo et al, 2019 ;Dux et al, 2018 ). Therefore, they are important targets to inform breeding practices.…”

“…Karim and his colleagues found that SSRs in PLAG1‐CHCHD7 intergenic region can affect promoter activity and influence the binding of nuclear factor (Karim et al., 2011). Not only that, the SSRs of IGF2R and LEP genes in cattle may influence the milk traits, feed intake, milk fatty acid composition and metabolic state (Avondo et al., 2019; Dux et al., 2018). Therefore, they are important targets to inform breeding practices.…”

“…In humans, SSRs are associated with a variety of diseases, such as cancer and Kennedy's disease (Breza & Koutsis, 2019; Fujimoto et al., 2020; Vieira et al., 2016). In cattle and goats, they significantly affect growth traits, milk quality, and virus resistance (Avondo et al., 2019; Dux et al., 2018; Karim et al., 2011). However, few studies have considered the associations between SSRs and economic traits in indigenous Chinese pigs.…”

Associations between polymorphisms of SLC22A7, NGFR, ARNTL and PPP2R2B genes and Milk production traits in Chinese Holstein

“…Therefore, in recent decades, quantitative trait locus (QTL) mapping, candidate gene analysis, genome-wide association analysis and high-throughput sequencing techniques have been widely used in dairy cow breeding to identify the functional elements [6][7][8][9][10]. Moreover, studies have shown that SNPs (single nucleotide polymorphisms) in the gene can significantly affect the milk production traits of dairy cows [11][12][13][14][15].…”