Association of some virulence genes with antibiotic resistance among uropathogenic Escherichia coli isolated from urinary tract infection patients in Alexandria, Egypt: A hospital-based study

Alabsi, Mogeeb S.; Ghazal, Abeer; Sabry, Soraya A.; Alasaly, Monasr M.

doi:10.1016/j.jgar.2014.01.003

Cited by 13 publications

(13 citation statements)

References 24 publications

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“…Thus UPEC stains uses iron acquisition genes like aerobactin, aer [46]. In this study we found 109 (54.5%) uropathogenic E. coli isolates carries aerobactin (aer) genes which is similar to studies conducted in Romania, 54% [16]; Tunisia, 52% [12]; Egypt, 51% [34] and Poland, 52.6% [42]; but lower than studies conducted in South Korea, 81% [39] and Iran, 73.1% [45]. Currently, Siderophore proteins are under investigation for potential vaccine candidate against UTI [26].There was significant association between aer gene and suprapubic pain, flank pain and fever (p-0.017, p-0.040, p-0.029 respectively).…”

Section: Discussionsupporting

confidence: 84%

“…Pyelonephritis associated pili (pap) gene was found in 59 (29.5%) uropathogenic E. coli isolates which is comparable to study conducted in Iran, 30.2% [6]; Mexico, 24.7% [31]; Romania, 36% [16] and Brazil, 32% [32]; but lower than studies conducted in Iran, 50.4% [28], 57% [33] and Egypt 54% [34]. In this study, there was significant association between presence of pap gene and urine urgency (p-0.016), which was commonly observed clinical symptom in most UTI patients.…”

Section: Discussionsupporting

confidence: 47%

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Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

et al. 2020

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Background: Urinary tract infection (UTI) is a common cause of morbidity worldwide. Uropathogenic Escherichia coli (UPEC) bacteria are the major cause of urinary tract infections. UPEC strains derive from different phylogenetic groups and possess an arsenal of virulence factors that contribute to their ability to overcome different defense mechanisms and cause disease. The objective of this study was to identify phylogroup and virulence genes of UPEC among urinary tract infection patients. Methods: A cross sectional study was conducted from January 1, 2017 to October 9, 2017. E. coli bacteria were isolated from UTI patients using culture and conventional biochemical tests. Identification of phylogroup and genes that encodes for virulence factors was done using multiplex polymerase chain reaction (PCR). Data was processed and analyzed with SPSS version16.0 and Epi-info version 3.4.1 software. Results: The most common urologic clinical manifestation combinations in this study were dysuria, urine urgency and urgency incontinence. The frequent UPEC virulence gene identified was fimH 164 (82%), followed by aer 109 (54.5%), hly 103 (51.5%), pap 59 (29.5%), cnf 58 (29%), sfa 50 (25%) and afa 24 (12%).There was significant association between pap gene and urine urgency (p-0.016); sfa and dysuria and urine urgency (p-0.019 and p-0.043 respectively); hly and suprapubic pain (p-0.002); aer and suprapubic pain, flank pain and fever (p-0.017, p-0.040, p-0.029 respectively). Majority of E. coli isolates were phylogroup B2 60(30%) followed by D 55(27.5%), B1 48(24%) and A 37(18.5%). There was significant association between E. coli phylogroup B2 and three virulence genes namely afa, pap, and sfa (p-0.014, p-0.002, p-0.004 respectively). Conclusion: In this study the most frequent E. coli virulence gene was fimH, followed by aer, hly, pap, cnf, sfa and afa respectively. There was significant association between E. coli virulence genes and clinical symptoms of UTI. The phylogenetic analysis indicates majority of uropathogenic E. coli isolates were phylogroup B2 followed by phylogroup D. Phylogroup B2 carries more virulence genes. Hence, targeting major UPEC phylogroup and virulence genes for potential vaccine candidates is essential for better management of UTI and further research has to be conducted in this area.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 47%

Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

et al. 2020

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“…This is similar to findings from studies done in other countries such as India (50, 59 and 68%) (Ranjini et al, 2015;Kothari and Sagar, 2008;Tambekar et al, 2006) and Madagascar (67%) (Randrianirina et al, 2007). Another study performed in Egypt reported that E. coli was in 36% of UTIs patients (Alabsi et al, 2014). A study performed in South Africa revealed that E. coli was present in 75% of uncomplicated and 59% of complicated UTIs and it was similar to this study (Agpaoa et al, 2015).…”

Section: Discussionsupporting

confidence: 90%

“…In this study, 23 strains (47.9%) of E. coli isolates were normally resistant to most of the antibiotics showing multi-drug resistance (MDR). The antibiogram resistance pattern of the strains was: Amoxicillin (66.7%), Ampicillin (56.3%), (Alabsi et al, 2014). A very high degree of MDR of 82.5% among E. coli isolates was reported by Ranjini et al (2015).…”

Section: Discussionmentioning

confidence: 93%

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Evaluation of antibacterial activity of some non-steroidal anti-inflammatory drugs against Escherichia coli causing urinary tract infection

Ahmed¹,

El-Baky²,

Ahmed³

et al. 2016

Afr. J. Microbiol. Res.

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Extensive use of antibiotics for urinary tract infections has led to the emergence of drug-resistant microorganisms and one solution to this problem is to search for non-antibiotic compounds that exert anti-bacterial activity through different mechanisms such as non-steroidal anti-inflammatory drugs (NSAIDs). In this study, out of 100 urine samples; 48 Escherichia coli strains were detected, 47.9% were multi-drug resistant. The antibiogram resistance pattern of the strains was carried out by agar dilution method. Diclofenac sodium, indomethacin, aspirin and ibuprofen were tested against the E. coli isolates. Diclofenac sodium showed the lowest MIC 50 and MIC 90 ; 8 and 256 g/ml, respectively. Aspirin showed MIC 50 of 64 g/ml, while both indomethacin and ibuprofen showed MIC 50 of 256 g/ml. Indomethacin, aspirin and ibuprofen showed the same MIC 90 of 1024 g/ml. The combined effects of the four NSAIDs and five antibiotics (Amoxicillin, Augmentin, Cefotaxime, Ciprofloxacin and Gentamicin) were tested on five resistant clinical E. coli strains by checkerboard dilution technique. All the tested NSAIDs significantly reduced the minimum inhibitory concentrations (MICs) of antibiotics against the tested bacteria and fractional inhibitory concentration indices (FICIs) for this combination ranged from 0.03 to 0.5. In this study, leakage of intracellular components suggests that the effect of NSAIDs on E. coli could be the formation of pores in the plasma membrane and scanning electron microscopy (SEM) observations confirmed the damage to the structural integrity of the tested bacteria. In conclusion, NSAIDs showed antibacterial activity against E. coli causing urinary tract infections (UTIs), a combination of them and antibiotics exhibited good synergism and the mechanism of their action was by damaging the bacterial cell membrane.

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Comparative Study of ESBL Production Among Uropathogenic Escherichia coli Clinical Isolates from Pre- and Post-menopausal Women in Egypt

Ali

Yakout

2021

Curr Microbiol

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Association of some virulence genes with antibiotic resistance among uropathogenic Escherichia coli isolated from urinary tract infection patients in Alexandria, Egypt: A hospital-based study

Cited by 13 publications

References 24 publications

Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

Evaluation of antibacterial activity of some non-steroidal anti-inflammatory drugs against Escherichia coli causing urinary tract infection

Comparative Study of ESBL Production Among Uropathogenic Escherichia coli Clinical Isolates from Pre- and Post-menopausal Women in Egypt

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