Association of surfactant protein D with pulmonary metastases from colon cancer

Abstract

Surfactant protein D (SP-D) is a member of the collectin family of proteins, which is secreted by airway epithelial cells. SP-D serves an important role in the immune system and in the inflammatory regulation of the lung. SP-D was recently found to suppress lung cancer progression by downregulating epidermal growth factor signaling. However, the relationship between SP-D and pulmonary metastases from colon cancer remains unknown. The present study aimed to determine whether SP-D may suppress the development of the mouse rectal carcinoma cell line, CMT93, in vitro . The present study investigated the effect of SP-D on pulmonary metastases from colon cancer in vivo using SP-D knockout mice. A wound healing assay and cell invasion assay revealed that SP-D suppressed the proliferation, migration and invasion of CMT-93 cells. After injection of CMT-93 cells into the tail vein, SP-D knockout mice were significantly more susceptible to developing pulmonary metastases than C57/BL6 mice (control). Moreover, a novel cell line (CMT-93 pulmonary metastasis; CMT-93 PM) was established from the lesions of pulmonary metastases in C57/BL6 mice following injection of CMT93 into the tail vein. CMT-93 PM exhibited more robust invasion and proliferation compared to CMT93, which was unaffected by exposure to SP-D. A higher incidence of pulmonary metastases was detected following injection of CMT93 PM into the tail vein of C57/BL6 mice compared with CMT-93. Consequently, SP-D may be involved in the pathogenesis of pulmonary metastases from colon cancer.

Effect of ultrasound-guided stellate ganglion block on lung protection for patients undergoing one-lung ventilation

“…Therefore, the change in SP-A is an important indicator for observing lung damage. Tajima et al pointed out that serum SP-A levels accurately reflected the extent of lung tissue damage and played an important role in the pathogenesis and diagnosis of noninfectious lung injury [37]. The results in this study demonstrated that the SP-A of the three groups were increased to varying degrees after OLV, indicating OLV caused damage to the lung tissue, and that there was a synergistic correlation between lung injury and inflammatory response and oxidative stress reaction.…”

Identification of hub genes and pathways in lung metastatic colorectal cancer

“…Surfactant protein D (also known as SFTPD or SP-D), encoded by the SFTPD gene, is a collagenous glycoprotein that resides in the lungs and extra-pulmonary tissues [51]. To date, only one study has reported that SFTPD is negatively associated with pulmonary metastases in CRC [52]. However, our in vitro and in vivo results showed that SFTPD promotes cellular proliferation, migration, and invasion and further enhanced CRC cell lung metastasis.…”

Profilin 2 isoform expression is associated with lung metastasis of colorectal cancer according to a comprehensive gene expression study using a mouse model

“…The molecular mechanisms underlying lung metastasis in CRC have been extensively studied (8,9,12,13,(20)(21)(22)(23). However, the number of patients included in these studies was insufficient to obtain conclusive results owing to the heterogeneity of metastatic tumors (8,12,21).…”

“…Cell culture. The CMT93-PM cell line was established in a previous study (13). Briefly, CMT93 cells (CCL-223; ATCC, Manassas, VA, USA) were injected into the tail veins of six-week-old female C57BL/6 mice.…”