Association of systemic antibody response against polyethylene terephthalate with inflammatory serum cytokine profile following implantation of differently coated vascular prostheses in a rat animal model

Abstract

Experimental studies demonstrated antibodies against matrix and coating of polyester‐based vascular prostheses. Thus, this study examined associations of these antibodies with serum cytokines (IL‐2, IL‐4, and IL‐10) and local inflammatory reactions. Rats (n = 8/group) intramuscularly received prosthesis segments [PET‐C, PET‐G, and PET‐A groups: polyethylene terephthalate (PET)‐based prostheses coated with bovine collagen and gelatin or human serum albumin, respectively; uncoated polytetrafluoroethylene‐based (PTFE) prosthesis], with sham‐operated controls. Blood was drawn pre‐operatively and weekly until day 22. Polymer‐specific or coating‐specific antibodies and cytokines were detected by enzyme immunoassays, inflammatory reactions were immunohistochemically evaluated on day 23. Polymer‐specific antibodies were detected in all PET‐groups using uncoated PET as antigenic target, but not for PTFE or controls, coating‐specific antibodies only for PET‐A. IL‐10 was increased in all PET‐groups and correlated with polymer‐specific antibodies for PET‐G and PET‐A. IL‐2 was increased for PET‐A, but overall correlated with PET‐specific antibodies. IL‐4 remained unchanged in all groups. Intense local inflammatory reactions (ED1+/ED2+ macrophages and T lymphocytes) were found within all PET‐groups, but only minor for PTFE or controls. In conclusion, PET‐specific antibodies were associated with increased IL‐10 and along with concurrent coating‐specific antibodies also with increased IL‐2, indicating a specific T cell response. Thus, matrix and/or coating of polymeric vascular prostheses elicit distinct systemic immune reactions, probably influencing local inflammatory reactions.

Quantitative analysis of trabecular bone tissue cryosections via a fully automated neural network-based approach

“…HE staining was performed according to standard protocols [ 23 , 24 ]. Sections were fixated in ice-cold acetone and dried for at least 30 minutes at 37°C.…”

Material-specific binding peptides empower sustainable innovations in plant health, biocatalysis, medicine and microplastic quantification

“…Recently, immune response and generation of antibodies against synthetic plastic, such as PET, was detected in rats with polymer prosthesis segments implanted. 378 Moreover, production of polyclonal polystyrene-binding antibodies after immunization of rabbits with polystyrene was reported. 379 Generated anti-PS antibodies did not bind to polypropylene, providing confirmation of their specificity.…”