Association of T869C gene polymorphism of transforming growth factor-β1 with low protein levels and anthropometric indices in osteopenia/osteoporosis postmenopausal Thai women

Abstract

ABSTRACT. Osteoporosis is the most common metabolic bone disease; it is an important health problem among postmenopausal women. We evaluated the association of three polymorphisms, T869C, C-509T and G915C, of the TGF-β1 gene with bone mineral density (BMD) serum TGF-β1 levels in 278 postmenopausal female osteopenia/osteoporosis subjects and 95 postmenopausal female control subjects. Serum TGF-β1 levels were significantly lower in osteopenia/ osteoporosis subjects than in control subjects. Serum TGF-β1 levels of the CT+CC (T869C) genotype group were significantly lower in osteopenia/osteoporosis subjects than in control subjects (11.3 vs 15.8 ng/mL). There was a significant difference in the CT+CC (T869C) genotype frequencies between the osteopenia/osteoporosis and control subjects (74.18 vs 60.22%; OR = 1.90, 95%CI = 1.16-3.12). In the age group of more than 50 years, subjects with the TC+CC genotype of T869C polymorphism had significantly increased risk of osteopenic/ osteoporotic bones at L1 (OR = 2.36, 95%CI = 1.37-4.07), L2 (OR = 1.71, 95%CI = 1.01-2.90), L3 (OR = 2.21, 95%CI = 1.23-3.98), L4 (OR = 1.74, 95%CI = 1.00-3.03) and the femoral neck (OR = 1.80, 95%CI = 1.04-3.12). The CT+CC genotype of the T869C polymorphism of the TGF-β1 gene was found to be associated with lower serum TGF-β1 in osteopenia/osteoporosis subjects and increased risk of osteopenic and osteoporotic fracture at L1-4, femoral neck and total hip in postmenopausal Thai women. Logistic regression analysis showed that T869C polymorphism is a significant risk factor for osteopenia/ osteoporosis. We concluded that T869C polymorphism of the TGF-β1 gene has an impact on decreased serum TGF-β1 levels and influences susceptibility to osteopenia/osteoporosis in Thai women.

The Transforming Growth Factor-β1 (TGF-β1) Gene Polymorphisms (TGF-β1 T869C and TGF-β1 T29C) and Susceptibility to Postmenopausal Osteoporosis

“…In our meta-analysis, significant association was observed between TGF-β1 T869C polymorphism and PMOP risk, which was consistent with 2 studies 15 , 20 ; however, another 2 studies 17 , 18 reported opposite results. In our opinion, 2 factors may be contributed to these controversial results.…”

“…A total of 8 studies 13 , 15 – 21 involving 1851 cases and 2247 controls eventually satisfied the eligibility criteria (Figure 1 ). Three studies 15 , 17 , 18 reported both alleles and genotypes of TGF-β1 T869C polymorphism and only 1 study 20 reported the TT and CT/CC in both case and control groups. With regard to TGF-β1 T29C polymorphism, there were 4 studies 13 , 16 , 19 , 21 reporting both alleles and genotypes of this gene polymorphism.…”

“…With regard to TGF-β1 T29C polymorphism, there were 4 studies 13 , 16 , 19 , 21 reporting both alleles and genotypes of this gene polymorphism. Furthermore, 6 studies 13 , 15 , 16 , 18 – 20 were performed in Asian populations and another 2 studies 17 , 21 were performed in Caucasian patients. The general demographic characteristics of studies included in this meta-analysis are summarized in Table 1 .…”

“…Among these genetic factors, TGF-β1 that plays an important role in the regulation of both bone formation and resorption, tissue recycling, and immune response has been widely studied in both Asian and Caucasian patients. 13 , 15 – 21 Utennam et al 20 evaluated the association of T869C, C-509T, and G915C of the TGF-β1 gene with BMD serum TGF-β1 levels in 278 postmenopausal female osteoporosis subjects and 95 postmenopausal female control subjects and they concluded that T869C polymorphism of the TGF-β1 gene had an impact on decreased serum TGF-β1 levels and influenced susceptibility to osteopenia/osteoporosis in Thai women, which was consistent with the results of studies by Yamada et al 13 , 15 and Lau et al 19 Tural et al 17 investigated the association between osteoporosis and TGF-β1 polymorphism in 146 osteoporotic and 97 healthy control women. However, they did not found any difference between the groups regarding TGF-β1 genotype distribution and allele frequencies.…”

Association between transforming growth factor-beta 1 gene single nucleotide polymorphisms and knee osteoarthritis susceptibility in a Chinese Han population

“…Previous studies have also identified a significant association between the CT genotype of SNP rs1800469 and the risk of OP in Thai women. 32 However, no studies elucidated the association between SNP rs1800469 and the risk of knee OA. Thus, the present study investigated the associations between CT and TT genotypes of rs1800469 and the risk of knee OA, but the results were negative.…”