Association of taurine with ovarian follicular steroids and postpartum anestrus condition in Murrah buffaloes

Taurine dynamics in serum during the oestrous cycle in buffaloes

“…They could be simple amino acids, sugars, fatty acids or complex glycoproteins, hormones, etc. In our previous study (Behera et al., 2020), the level of taurine was reported to be lower in the plasma during postpartum anestrus conditions in buffaloes. Therefore, to explore further, the present study was targeted to understand serum taurine dynamics during the oestrous cycle in buffaloes.…”

“…In addition, it is a known fact that estrogens are synthesised from their precursors like testosterone. In our previous study, we found a positive correlation between taurine and testosterone in the follicular fluid of developing ovarian follicles (Behera et al, 2020). Therefore, a greater concentration of taurine just before estrus (proestrus) could also contribute to the greater concentration of testosterone during proestrus, which is the F I G U R E 8 ROC curve analysis for serum proline concentration.…”

“…After the oestrous cycle stages were determined, four samples representing all four stages of the oestrous cycle were taken from each animal. The serum samples were deproteinised by adding 400 μL of methanol to 100 μL serum (sample:methanol::1:4) as per the protocol established in our lab earlier (Behera et al., 2020) with little modifications from a previous publication (Mu et al., 2019). The mixture was incubated at room temperature for about 6 min and centrifuged at 8000 g for 5 min at 4°C.…”

“…After the spots dried, 10 μL of 1 mg/mL proline was added to the same spots externally. Proline solution was used as a biological control to minimise the plate variations as given in our previous protocol, which was performed on ovarian follicular fluid and the blood plasma from post-partum buffaloes (Behera et al, 2020). Along with the serum samples, 10 μL of standard (1 mg/ mL) taurine was also loaded on the plate as a separate spot to compare the relative spots of taurine obtained from the serum samples.…”

“…Further, taurine also showed a positive correlation (0.75) with testosterone and a negative correlation (−0.77) with oestrogen in the developing ovarian follicles of buffaloes, indicating the importance of taurine and testosterone during the development of ovarian follicles. Apart from ovarian follicular fluid, a significantly lower level of taurine in the plasma of buffalo during post‐partum anestrus condition shows not only taurine's role in maintaining normal reproductive cyclicity in buffaloes (Behera et al., 2020) but also highlights the necessity to understand the plasma or serum dynamics of taurine during the reproductive cycle of buffaloes. However, serum taurine dynamics during the buffalo oestrous cycle were not reported.…”