Association of the human aryl hydrocarbon receptor repressor (AhRR)‐c.565C>G transversion with male infertility: A case‐control study from Iran

Abstract

Dioxins (eg, 2,3,7,8-tetrachlorodibenzo-p-dioxin/TCDD), as environmental endocrine disruptors and toxic carcinogens, can affect male reproductive health. The influence of dioxins is mediated via the aryl hydrocarbon receptor (AhR) pathway and its repressor (AhRR). In this study, we investigated the association of AhRR-c.565C>Gtransversion polymorphism with male infertility. In a hospital-based case-control study, 221 semen samples (111 infertile and 110 healthy controls) based on World Health Organization guidelines were collected from in vitro fertilization centers of Babol, Iran. The AhRR-c.565C>G (rs2292596) polymorphism was genotyped using a polymerase chain reaction-restriction fragment length polymorphism analysis. The difference in the allele frequency of AhRR-c.565C>G transversion polymorphism did not reach a significant level. The genotype frequency was statistically significantly different between fertile and infertile men. We found that polymorphism rs2292596

Association of AhRR rs2292596 with male infertility in 422 Vietnamese individuals

“…Additionally, the study also revealed a negative association between the appearance of genotype Ala/Ala and sperm count. Inconsistent with the study on Japanese population, an investigation of an Iranian population consisting of 111 patients and 110 healthy men also proposed that the wild-type allele Pro185 is associated with both susceptibility and severity of infertility, and can be used as a genetic marker in the prediction of male infertility (Mohammad-Hasani et al, 2019). Meanwhile, in the present study, no association between polymorphism AhRR rs2292596 and male infertility was recognized in Vietnamese population with any model (p > 0.05).…”

AHR-dependent genes and response to MTX therapy in rheumatoid arthritis patients

“…Hung et al concluded that genotype CC is associated with the more active form of AHRR and lower repressor activity over AhR whereas GG—with the less active form of AhRR [ 24 ]. Interestingly, more severe forms of endometriosis were found in those with AhRR genotype GG (Ala/Ala) in rs2292596 [ 32 ] whereas male infertility seems to be associated with wild type CC [ 33 , 22 ]. Accordingly, with this hypothesis, our NGS analysis has shown that poor MTX responders in RA patients group more frequently carried alternative G allele which supposed to be translated as the less active form of AHRR and higher inducibility of CYP1A2 [ 34 ].…”

“…So far c.1933G > C (rs34453673) has only been mentioned in the study on CYP1A2 activity, where the association of this SNP was not proved [ 20 ]. In the case of c.565C > G (rs2292596), the association of this variant with RA risk in the Chinese Han population has been analyzed [ 21 ] and in few other studies associated with infertility [ 22 , 23 ] or xenobiotic metabolism [ 20 , 24 ].…”

Analysis and Comparison of Zinc Micro Element Concentration Present in Human Seminal Plasma of Asthenospermia and Normospermia Conditions Using Atomic Absorption Spectroscopy for Male Infertility Diagnosis Related to Motility Issues

“…Zn content in human semen was 85 to 90 times higher in seminal plasma than that of blood. Zinc acts as a shield for sperm cells while they enter the female reproductive tract (Mohammad-Hasani, Colagar, and Fallah 2019), protecting them from chromosomal damage (Kovacik et al 2018;S. Vickram et al 2021).…”