Association of the CDH1 Gene Variant (Promoter -160 C/A) with Breast Cancer and Its Linking with Prognostic and Clinical Features of Patients

Abstract

Breast cancer (BC) is one of gynecological cancers worldwide. Because E-cadherin is essential protien for cells connection and maintains on original shape for epithelial tissue, this study supposed that CDH1 -160 C/A SNP is linked with increased BC hazard and assess linking between this SNP genotypes and prognostic and clinical features of BC females. Subjects were divided into 75 BC cases and 50 healthy controls without family history to any cancer. This SNP variants was determined by PCR-RFLP technique. This study recorded a much increase in number of women up 45 years (p=0.0008). In addition, women who had BC family history had a significant increase (p=<0.00001) in BC incidence. These results found that cases with ductal carcinoma were significantly (p=<0.00001) more prevalent among BC patients. Also, distribution of BC appeared mostly in cases with grade II, tumor stage III that involved lymph node metastasis with significant differences (0.0003, 0.00005, <0.00001 respectively). This study explained that minor A allele of this SNP much risen in BC females compared to controls (p=0.005). Also, these results reported that C/A and A/A genotypes were correlated with increasing of patients ages as well as positive family history and ductal carcinoma with significant differences (p=0.03, p=0.0003, p=0.002). In sum, this study determined that the CDH1 -160 C/A SNP was significantly increased BC hazard in Iraqi females. moreover, mutant A allele was significantly correlated with cases who had positive family history, ductal cancer and age over 45 years.