Association of the KiSS1 gene with litter size in Cyprus and Iraqi black goats

Abstract

Aim: The study investigated the genetic polymorphism of the kisspeptin (KiSS1) gene and its relationship with litter size in Cyprus and Iraqi black goats. Materials and Methods: Blood samples (n=124) were collected from the two goat breeds reared at the Agricultural Research-Ruminant Research Station Breeding Station, Baghdad, Iraq. Genomic DNA was isolated using a DNA extraction kit. Polymerase chain reaction (PCR) was used to amplify the KiSS1 gene. All PCR products were sequenced and samples were used for further analysis using NCBI-Blast online on the exon 1 (595 bp) region of the KiSS1 gene. Results: The results of this study revealed a significantly (P<0.05) larger litter size of the Cyprus goat breed than in the Iraqi black goats in the first and second parity. Three (893G/C, 973C/A, and 979T/G) substitutions relative to the KiSS1 gene reference sequence (GenBank ID: J × 047312.1, KC989928.1) were identified. Only the mutation g893G>C was identified as a single nucleotide polymorphism (SNP) associated with litter size. Furthermore, the average alleles in KiSS1 gene of both types of goats 0.567 and 0.3715 GG, were recorded. The genotyping at locus g893C>G was demonstrating domination of fecundity quality litter size, Both genotypes SNP of GC were classified at this marked region of KiSS1 gene. Conclusion: The study concluded that the role of the KiSS1 gene in fecundity, revealing the status of this gene as an indicator in the assisted of caprine breeding selection.

Induction Estrus in Local Anestrum Bitches by using GnRH , PMSG and hCG Combination

“…The results of the number of offspring agree with [20] who claimed that the majority of his research's data came from huge litters, with a mean litter size of roughly 7 puppies and just three stallions having just one youngster; the rest all had 5 or more. Additionally said that increased follicular recruitment in response to pituitary stimulation by the GnRH agonist may have contributed to the large litter size in the majority of fawns [21]. In addition, [13] eCG and hCG treatment resulted in a newborn baby with 38 whelps being reported.…”

Association of g.331T>C and g.1909T>C locus in KISS1 gene with fecundity traits of Kacang and Boerka goats

“…El-Tarabany et al ( 2017) stated that the SNP of the KISS1 gene in intron 1 was significantly associated with litter size in Damascus and Zaribi goats. The research results by Rahawy and AL-Mutar (2021) stated that the diversity of the KISS1 gene was significantly associated with litter size in Cyprus and Iraqi black goats. High levels of KISS1 gene expression were found in the pituitary of productive goats and were associated with litter size in Jintang black goats (Zheng et al 2018).…”

“…Furthermore, Cao et al (2010) also discovered a link between allele C of the 296 loci and allele deletion of the 1960-1977 locus in the caprine KiSS1 gene and large litter size in the Jining Grey breed. Rahawy and Al-Mutar (2021) stated that a mutation in g.893G>C was identified as an SNP polymorphism associated with litter size in Cyprus and Iraqi black goats. In Indian goat breeds, polymorphism of SNPs G296C at intron 1 was associated with litter size (Maitra et al 2014).…”

“…However, the key genes in caprine that control litter size have not been completely revealed. Numerous genes regulate litter size (Rahawy and Al-Mutar 2021).…”

“…An et al (2013) and El-Tarabany et al (2017) discovered a polymorphism in the number of KISS1 gene loci correlating with litter size. The single nucleotide polymorphism (SNP) g893G>C was identified as being associated with litter size in Iraqi black goats (Rahawy and AL-Mutar 2021). Therefore, it was essential to investigate the interaction of several loci for the standardization model of goat farming (Ahlawat et al 2015).…”