Stroke is a condition that negatively affects the quality of life in all societies and is one of the leading causes of mortality in adults. It is the second most common cause of death and disability worldwide. Acute ischemic stroke leads to severe physical, social, psychological, and economic devastation. Early and accurate diagnosis of these patients in the emergency department is crucial for timely treatment. This study aimed to investigate the course of the pan-immune inflammation value (PIV), systemic immune-inflammation index (SII), and systemic inflammatory response index (SIRI), which are discussed as new inflammatory markers in patients diagnosed with acute stroke. Materials and Methods: This retrospective study compared inflammatory markers obtained from the complete blood count results of patients diagnosed with acute ischemic stroke in the emergency department between 01.01.2022 and 01.03.2024 with those of healthy individuals. Findings: In our study, 201 patients with acute ischemic stroke and 100 healthy individuals were compared. The results demonstrated that inflammatory markers, including the Pan-Immune Inflammation Value (PIV), Systemic Immune-Inflammation Index (SII), and Systemic Inflammatory Response Index (SIRI), were significantly higher in the stroke group compared to the control group (p < 0.05). Specifically, neutrophil, CRP, PIV, SII, SIRI, and NLR values were elevated in the ischemic stroke group, while lymphocyte and hemoglobin levels were higher in the control group. Conclusion: This study highlights the potential of PIV, SII, and SIRI as novel biomarkers for predicting inflammation and prognosis in acute ischemic stroke patients. These markers may serve as cost-effective and practical tools for early diagnosis and risk stratification in clinical settings. Further studies with dynamic evaluations are needed to explore the long-term prognostic value of these markers.