Association of the transcriptional response of soybean plants with soybean mosaic virus systemic infection

Abstract

Compatible virus infection induces and suppresses host gene expression at the global level. These gene-expression changes are the molecular basis of symptom development and general stress and defence-like responses of the host. To assess transcriptional changes in soybean plants infected with soybean mosaic virus (SMV), the first soybean trifoliate leaf, immediately above the SMV-inoculated unifoliate leaf, was sampled at 7, 14 and 21 days post-inoculation (p.i.) and subjected to microarray analysis. The identified changes in gene expression in soybean leaves with SMV infection at different time points were associated with the observed symptom development. By using stringent selection criteria (¢2-or ¡"2-fold change and a Q value of ¡0.05), 273 (1.5 %) and 173 (0.9 %) transcripts were identified to be up-and downregulated, respectively, from 18 613 soybean cDNAs on the array. The expression levels of many transcripts encoding proteins for hormone metabolism, cell-wall biogenesis, chloroplast functions and photosynthesis were repressed at 14 days p.i. and were associated with the highest levels of viral RNA in the host cells. A number of transcripts corresponding to genes involved in defence were either downregulated or not affected at the early stages of infection, but upregulated at the late stages, indicating that the plant immune response is not activated until the late time points of infection. Such a delayed defence response may be critical for SMV to establish its systemic infection.

Detection, Understanding and Controlof Soybean Mosaic Virus

“…Theses changes were associated with the highest levels of SMV genomic RNA in the host cells and the progression of mosaic and vein clearing symptoms (Babu et al, 2008). The expression levels of a number of transcripts corresponding to genes involved in defense were either downregulated or not affected at the early stages of infection, but upregulated at the late stages (Babu et al, 2008). These data suggest that in susceptible cultivars, the plant immune response is not activated until the late time point of infection and such a delayed defense response may be critical for SMV to establish its systemic infection.…”

“…A number of transcripts encoding proteins for hormone metabolism, cell-wall biogenesis, chloroplast functions and photosynthesis were shown to be repressed at 14 days post infection (Babu et al, 2008). Theses changes were associated with the highest levels of SMV genomic RNA in the host cells and the progression of mosaic and vein clearing symptoms (Babu et al, 2008). The expression levels of a number of transcripts corresponding to genes involved in defense were either downregulated or not affected at the early stages of infection, but upregulated at the late stages (Babu et al, 2008).…”

“…Such compatible virus infection often induces and suppresses host gene expression at the global level (Whitham et al, 2006). Babu et al (2008) assessed transcriptional changes in susceptible soybean cultivar Williams 82 infected by SMV using microarray. A number of transcripts encoding proteins for hormone metabolism, cell-wall biogenesis, chloroplast functions and photosynthesis were shown to be repressed at 14 days post infection (Babu et al, 2008).…”

“…Babu et al (2008) assessed transcriptional changes in susceptible soybean cultivar Williams 82 infected by SMV using microarray. A number of transcripts encoding proteins for hormone metabolism, cell-wall biogenesis, chloroplast functions and photosynthesis were shown to be repressed at 14 days post infection (Babu et al, 2008). Theses changes were associated with the highest levels of SMV genomic RNA in the host cells and the progression of mosaic and vein clearing symptoms (Babu et al, 2008).…”

Proteomics-Based Analysis of <i>Phalaenopsis amabilis</i> in Response toward <i>Cymbidium</i> Mosaic Virus and/or <i>Odontoglossum</i> Ringspot Virus Infection

“…However, there are few studies on pathogenesis to P. amabilis and none of these reports used proteomics-based analysis to examine the mechanisms altered by infection with the exception of recent investigations with soybean [7] and cucumber [8]. Proteomics is currently a powerful tool to systematically analyze the cellular protein expression profiles and posttranslational modifications under virus invasion in the host of rice [9][10][11], tobacco [12], soybean [13], tomato [14] and Arabidopsis thaliana [15] respectively.…”