Association of TNF-α G308A gene polymorphism in essential hypertensive patients without type 2 diabetes mellitus

Abstract

ABSTRACT. This study aims to investigate the effects of tumor necrosis factor alpha (TNF-α) G308A gene polymorphism on essential hypertension (EHT) with or without type 2 diabetes mellitus (T2DM). The project was conducted on buccal epithelial and blood cells for case and control patients, respectively. Epithelial cells were obtained from the inner part of the cheeks. Techniques including DNA extraction, polymerase chain reaction (PCR), and restriction fragment length polymorphism (RFLP) were utilized to assess biomarkers of DNA damage. Our results demonstrated significant differences between wild and mutated genotypes among EHT patients without T2DM. We also found a significant association between wild and mutated allele frequencies in EHT patients (P < 0.05). Clinical characteristics between the groups (EHT with or without T2DM and 18975 Association of TNF-α G308A gene polymorphism in EHT without T2DM ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 18974-18979 (2015) controls) showed statistically significant association (P < 0.05). Overall, we show that G308A polymorphism of the TNF-α gene may be a significant genetic risk factor for EHT without T2DM patients in Malaysia.

Association between TNF-a promoter -308G/A polymorphism and essential hypertension in the Asian population: A meta-analysis

“…30,31 Increasing numbers of scholars are exploring scholars the relationship between TNF-a -308G/A polymorphism and EH, but the conclusions are not consistent. [18][19][20][21][22][23][24][25][26] A single study may not draw a comprehensive and reliable conclusion due to the low effectiveness of the tests used. In addition, because of the different frequencies (TNF-a -308G/A) of the different regions and ethnic groups, the extrapolation of the foreign research is limited.…”

“…17 Finally, a total of nine casecontrol studies were identified, including 1437 cases and 1487 controls. [18][19][20][21][22][23][24][25][26] These nine studies encompassed one Caucasian and eight Asian studies. The characteristics of the selected studies and the numbers of subjects are shown in Table 1.…”

Obstructive sleep apnea and hypertriglyceridaemia share common genetic background: Results of a twin study

“…is associated with higher plasma TNF-alpha levels and this polymorphism is a predisposition of OSA (Sun et al, 2015) and hypertension (Ghodsian et al, 2015).…”

“…Polymorphisms in the leptin receptor (LTPR) gene were significantly associated with the risk of hypertension (Lian, Tang, Xie, & Chen, ), T2DM (Su et al, ) and OSA (Xu, Liu, Li, & Liu, ). Finally, tumor necrosis factor‐alpha (TNF‐alpha) G308A is associated with higher plasma TNF‐alpha levels and this polymorphism is a predisposition of OSA (Sun et al, ) and hypertension (Ghodsian et al, ).…”

Effects of ginsenoside Rg1 on glucose metabolism and liver injury in streptozotocin-induced type 2 diabetic rats

“…Diabetes mellitus (DM) is an endocrinal and metabolic disease featuring high blood glucose (de Azevêdo Silva et al, 2015;Ghodsian et al, 2015). Following cardiovascular disease and cancer, DM is the third serious chronic, non-infectious disease threatening human health.…”