Association of Treponema spp. with canine periodontitis

Nordhoff, Marcel; Rühe, Bärbel; Kellermeier, Claudia; Moter, Annette; Schmitz, Rose; Brunnberg, Leo; Wieler, Lothar H.

doi:10.1016/j.vetmic.2007.09.011

Cited by 28 publications

(20 citation statements)

References 30 publications

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“…In this context, there is a variety of qualitative or quantitative studies evaluating Treponema species involved human periodontitis or healthy sites (Willis et al 1999, Sato & Kuramitsu 2000, Asai et al 2002, as found in dog periodontitis (Riviere et al 1996, Nordhoff et al 2008; however, Treponema denticola prevalence is more often associated to increased severity of periodontitis in humans and dogs.…”

Section: Discussionmentioning

confidence: 99%

Treponema denticola in microflora of bovine periodontitis

et al. 2015

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RESUMO.-[Treponema denticola na microbiota da periodontite bovina.] A periodontite bovina é um processo infeccioso purulento e progressivo associado à presença de biofilme subgengival anaeróbio estrito e epidemiologicamente relacionada ao manejo do solo em amplas áreas geográficas do Brasil. O trabalho teve por objetivo detectar espécies de Treponema presentes na bolsa periodontal de bovinos com lesões de profundidade maior que 5mm e do sulco gengival de animais com idade de 6 a 24 meses e considerados periodontalmente sadios. Os materiais foram colhidos por meio de cones de papel, após a remoção do biofilme supragengival, e mantidos sob congelamento (-80°C) até a extração do DNA e realização da reação em cadeia da polimerase (PCR) com o emprego de iniciadores de T. amylovorum, T. denticola, T. maltophilum, T. medium e T. vincentii. Na bolsa periodontal de 73% (19/26) dos animais foi possível detectar diretamente, pela PCR, a presença de Treponema amylovorum, de 42,3% (11/26) T. denticola e de 54% (14/26) T. maltophilum. Dos 25 sítios sadios, em 18 (72%) foi possível identificar T. amylovorum, em dois (8%) T. denticola e em oito (32%) T. maltophilum. Treponema medium e T. vincentii não foram detectados nas 51 amostras avaliadas. A presença de Treponema amylovorum, T. maltophilum na microbiota subgengival, e em especial do amplamente reconhecido periodontopatógeno T. denticola, Treponema denticola in microflora of bovine periodontitis Periodontitis in cattle is an infectious purulent progressive disease associated with strict anaerobic subgingival biofilm and is epidemiologically related to soil management at several locations of Brazil. This study aimed to detect Treponema species in periodontal pockets of cattle with lesions deeper than 5mm in the gingival sulcus of 6 to 24-month-old animals considered periodontally healthy. We used paper cones to collect the materials, after removal of supragingival plaques, and kept frozen (at -80°C) up to DNA extraction and polymerase chain reaction (PCR) using T. amylovorum, T. denticola, T. maltophilum, T. medium and T. vincentii primers. In periodontal pocket, it was possible to identify by PCR directly, the presence of Treponema amylovorum in 73% of animals (19/26), T. denticola in 42.3% (11/26) and T. maltophilum in 54% (14/26). Among the 25 healthy sites, it was possible to identify T. amylovorum in 18 (72%), T. denticola in two (8%) and T. maltophilum in eight (32%). Treponema medium and T. vincentii were not detected over all 51 evaluated samples. The presence of Treponema amylovorum, T. maltophilum and, in particular, the widely recognized T. denticola in subgingival microflora brings an original and potencially important contribution in studies of the bovine periodontitis.INDEX TERMS: Bovine periodontitis, periodontal disease, subgingival microflora, Treponema denticola.

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Section: Discussionmentioning

confidence: 99%

Treponema denticola in microflora of bovine periodontitis

et al. 2015

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“…Treponema denticola, on the other hand, has been reported to be associated with canine periodontitis and detectable in a significantly greater proportion in dogs with periodontal pocket depths < 5-mm. 22,23 It is interesting to compare the present findings with others that have used different types of formulations to treat periodontitis in canines. Films have been made from either methacrylic acid/methylmethacrylate copolymers, or ethyl methacrylate/ chlorotrimethylammonium methyl methacrylate copolymer to deliver clindamycin in a sustained manner.…”

Section: Discussionmentioning

confidence: 74%

Canine Periodontal Disease Control Using a Clindamycin Hydrochloride Gel

et al. 2011

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Stabilizing or reducing periodontal pocket depth can have a positive influence on the retention of teeth in dogs. A topical 2% clindamycin hydrochloride gel (CHgel) was evaluated for the treatment of periodontal disease in dogs. The CHgel formulation provides for the sustained erosion of the matrix, but also flows into the periodontal pocket as a viscous liquid, and then rapidly forms a gel that has mucoadhesive properties and also may function as a physical barrier to the introduction of bacteria. A professional teeth cleaning procedure including scaling and root planing was done in dogs with one group receiving CHgel following treatment. Periodontal health was determined before and after the procedure including measurement of periodontal pocket depth, gingival index, gingival bleeding sites, and number of suppurating sites. There was a statistically significant decrease in periodontal pocket depth (19%), gingival index (16%), and the number of bleeding sites (64%) at 90-days in dogs receiving CHgel. Additionally, the number of suppurating sites was lower (93%) at 90-days for the group receiving CHgel. The addition of CHgel effectively controlled the bacterial burden (e.g, Fusobacterium nucleatum) at both day 14 and 90. Gingival cells in culture were shown to rapidly incorporate clindamycin and attain saturation in approximately 20-minutes. In summary, a professional teeth cleaning procedure including root planning and the addition of CHgel improves the gingival index and reduces periodontal pocket depth.

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“…Previous studies of BDD in dairy cattle and beef cattle, and more recently CODD in sheep, show that in all DD lesions, one or more of the three cultivable DD treponeme phylogroups are present (6,12,13,19,23). However, although these phylogroups are consistently detected and isolated from cattle and sheep lesions, studies have failed to isolate these pathogenic treponemes from potential transmission vectors and infection reservoirs.…”

Section: Discussionmentioning

confidence: 99%

“…The primary causative agents of DD are considered to be the spirochetal bacteria, treponemes, with a polytreponemal etiology (11)(12)(13). Cloning bacterial 16S rRNA genes indicated five phylogroups of treponemes in BDD lesions from Germany (14).…”

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The Gastrointestinal Tract as a Potential Infection Reservoir of Digital Dermatitis-Associated Treponemes in Beef Cattle and Sheep

Sullivan¹,

Carter²,

Duncan

et al. 2015

Appl Environ Microbiol

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bDigital dermatitis (DD) is an important cause of lameness in dairy cattle worldwide. It has now been reported in beef cattle and also sheep (contagious ovine digital dermatitis [CODD]). Three Treponema phylogroups are consistently isolated from lesions, Treponema medium-like, Treponema phagedenis-like, and Treponema pedis. The gastrointestinal (GI) tract and feces are suggested sites of treponemal infection in dairy cattle; however, isolation of DD-associated treponemes from these areas has previously failed. This study surveyed gingival tissues, rectal tissues, and feces of beef cattle and sheep for the molecular presence (PCR) and isolation of the three cultivable DD-treponeme phylogroups. Of the sheep gingival (n ‫؍‬ 40) and rectal (n ‫؍‬ 40) tissues, 1/40 gingival tissues was positive for DD-associated treponemes (T. pedis), as were 3/40 rectal tissues (one containing T. medium-like and two containing T. pedis). No DD-associated treponeme DNA was amplified from beef cattle rectal tissues (n ‫؍‬ 40); however, 4/40 beef gingival tissues were positive for DD-associated treponemes (all containing T. phagedenis-like). A T. phagedenis-like DD-associated treponeme was isolated from the rectal tissue of a CODD symptomatic sheep. Beef cattle (n ‫؍‬ 41) and sheep (n ‫؍‬ 79) feces failed to amplify DD-associated Treponema DNA. Twenty-two treponemes were isolated from sheep feces; however, upon phylogenetic analysis, these clustered with the considered nonpathogenic treponemes. This study detected DD-associated treponemes in the GI tract tissues of sheep and beef cattle and successfully isolated a DD-associated treponeme from ruminant rectal tissue. This gives evidence that the GI tract is an important infection reservoir of DD-associated treponemes in multiple DD-infected species. Digital dermatitis (DD) is an ulcerative lesion of the digital skin (1), causing severe lameness in dairy cattle worldwide. The disease was first reported in dairy cattle in 1974, where it is known as bovine digital dermatitis (BDD) (1, 2). The disease more recently has been reported in sheep (3, 4), where it is known as contagious ovine digital dermatitis (CODD), and now has been confirmed in beef cattle (5, 6).Over the last few decades, DD has been recognized as an important cause of bovine lameness (7) with serious animal welfare and economic implications, including reduced fertility and milk yield and an increase in the risk of culling (8-10). DD is now a worldwide problem, and controlling the disease on dairy operations has proven difficult due to there being no single effective treatment available. Furthermore, there are no data on treatment of DD in beef cattle, and treatment of CODD is difficult and rarely effective.The primary causative agents of DD are considered to be the spirochetal bacteria, treponemes, with a polytreponemal etiology (11-13). Cloning bacterial 16S rRNA genes indicated five phylogroups of treponemes in BDD lesions from Germany (14). Three Treponema phylogroups have been consistently isolated from dairy cattle les...

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Association of Treponema spp. with canine periodontitis

Cited by 28 publications

References 30 publications

Treponema denticola in microflora of bovine periodontitis

Treponema denticola in microflora of bovine periodontitis

Canine Periodontal Disease Control Using a Clindamycin Hydrochloride Gel

The Gastrointestinal Tract as a Potential Infection Reservoir of Digital Dermatitis-Associated Treponemes in Beef Cattle and Sheep

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