Association of Two Polymorphic Codons in P53 and ABCC1 Promoter with Prostate Cancer

Behfarjam, Farinaz; Rostamzadeh, Jalal; Zarei, Mohammad; Nikkhoo, Bahram

doi:10.15171/ijb.1096

Cited by 8 publications

(6 citation statements)

References 34 publications

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“…Cortez et al suggested that p53 mutants could intervene in the immune escape of tumors by regulating the expression of PD-L1 [ 24 ]. Moreover, p53 has been verified to mutate in prostate cancer [ 25 ]. Wig1, a transcriptional target of p53, encodes an unusual zinc finger protein that participates in post-transcriptional gene regulation.…”

Section: Discussionmentioning

confidence: 99%

eIF5B regulates the expression of PD-L1 in prostate cancer cells by interacting with Wig1

Xiao

Shi

et al. 2021

BMC Cancer

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Background Eukaryotic translation initiation factors (eIFs) are the key factors to synthesize translation initiation complexes during the synthesis of eukaryotic proteins. Besides, eIFs are especially important in regulating the immune function of tumor cells. However, the effect mechanism of eIFs in prostate cancer remains to be studied, which is precisely the purpose of this study. Methods In this study, three groups of prostate cancer cells were investigated. One group had its eIF5B gene knocked down; another group had its Programmed death 1 (PD-L1) overexpressed; the final group had its Wild-type p53-induced gene 1 (Wig1) overexpressed. Genetic alterations of the cancer cells were performed by plasmid transfection. The expression of PD-L1 mRNA was detected by quantitative real-time PCR (qRT-PCR), and the expressions of PD-L1 and eIF5B proteins were observed by western blot assays. Cell Counting Kit-8 (CCK-8), flow cytometry, Transwell and Transwell martrigel were used to investigated cell proliferation, apoptosis, migration and invasion, respectively. The effect of peripheral blood mononuclear cells (PBMCs) on tumor cells was observed, and the interaction between eIF5B and Wig1 was revealed by co-immunoprecipitation (CoIP) assay. Finally, the effects of interference with eIF5B expression on the growth, morphology, and immunity of the tumor, as well as PD-L1 expression in the tumor, were verified by tumor xenograft assays in vivo. Results Compared with normal prostate epithelial cells, prostate cancer cells revealed higher expressions of eIF5B and PD-L1 interference with eIF-5B expression can inhibit the proliferation, migration, invasion and PD-L1 expression of prostate cancer cells. Meanwhile, the cancer cell group with interference with eIF5B expression also demonstrated greater, apoptosis and higher vulnerability to PBMCs. CoIP assays showed that Wig1 could bind to eIF5B in prostate cancer cells, and its overexpression can inhibit the proliferation, migration, invasion and PD-L1 expression of cancer cells while promoting apoptosis. Moreover, interference with eIF5B expression can inhibit tumor growth, destroy tumor morphology, and suppress the proliferation of tumor cells. Conclusion eIF5B can promote the expression of PD-L1 by interacting with Wig1. Besides, interference with eIF5B expression can inhibit the proliferation, migration, invasion and immunosuppressive response of prostate cancer cells. This study proposes a new target, eIF5B, for immunotherapy of prostate cancer.

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Section: Discussionmentioning

confidence: 99%

eIF5B regulates the expression of PD-L1 in prostate cancer cells by interacting with Wig1

Xiao

Shi

et al. 2021

BMC Cancer

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“…It has even recently been found to be used in chest high-resolution computed tomography imaging findings of coronavirus disease 2019 (Covid-19) pneumonia (42). Cancer research has yielded good results through the study of gene expression as well as biotechnological methods (43)(44)(45)(46)(47)(48).…”

Section: Discussionmentioning

confidence: 99%

Application of intravoxel incoherent motion imaging in the study of migration and movement of cancer cells

Wang

Ahmed

2020

Cell Mol Biol (Noisy-le-grand)

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IVIM imaging technology can obtain physiological information of tissue cells and blood perfusion at the same time in one imaging process. Taking female patients in Shenzhen Maternal and Child Health Hospital affiliated to Southern Medical University as an example, the migration and movement of breast cancer cells and hepatocellular carcinoma cells are studied by intravoxel incoherent motion imaging. Apparent diffusion coefficient (ADC), true diffusion coefficient (D), pseudo-diffusion coefficient (D*) and perfusion fraction (f) are measured by the IVIM bi-index model, and statistical analysis is made. The results show that the IVIM bi-index model can truly reflect the diffusion of water molecules in breast mass-like lesions, and reflect the migration and movement of breast cancer cells. Especially D value has important reference significance for the research and diagnosis of breast cancer cells. IVIM bi-index model has limited diagnostic value for the migration and movement of common hepatocellular carcinoma cells, and ADC value may have higher diagnostic efficiency.

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“…The p53Arg72 protein persuades apoptosis faster and represses alteration more competently than the p53Pro72 protein ( 27 ). There have been reports showing possible involvement of P53c72 polymorphism in individuals’ susceptibility to cancers including mouth cancer, breast cancer, thyroid cancer, colorectal, and prostate cancer, which seem to have a mutation in this gene ( 9 , 11 , 13 , 14 , 24 ). The mentioned case-control studies showed the contribution of P53c72 polymorphism to the carcinogenesis although these studies were done with a small sample size and the results from these individual studies lacked consensus.…”

Section: Discussionmentioning

confidence: 99%

Association of p53 codon 72 Arg>Pro polymorphism and risk of cancer in Iranian population: A systematic review and meta-analysis

Roshani

Abdolahi

Rahmati

2017

Med. J. Islam. Republic Iran

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Background: Different studies have investigated the association between p53 codon 72 Arg>Pro polymorphism and cancer risk. Because of the lack of consensus of the results in individual studies, we conducted this meta-analysis by pooling all currently available case–control studies to estimate the effect of p53 codon 72 Arg/Pro polymorphism on cancer susceptibility in Iranian population. Methods: A comprehensive search was undertaken and primary data from all peer-reviewed journals indexed in PubMed, Google Scholar, Scopus, Magiran, Scientific Information Databank (SID), Iran Medex, and CAB abstract electronic were used to conduct this meta-analysis. We considered some exclusion and inclusion criteria to select the articles. Statistical heterogeneity was explored using the I-square. Publication bias was assessed graphically and statistically by Begg’s funnel plot and Egger test. All statistical analyses were performed using StatsDirect software and a two- tailed test. P-value less than 0.05 was considered statistically significant for any test. Results: Our dataset, which included 35 case-control studies, consisted of 2426 cancer cases and 2928 controls. Pooled OR and 95%CI indicated that codon 72 Arg>Pro polymorphism was not associated with odds of developing cancer among Iranian population in the dominant model (Pro/Pro+Arg/Pro vs. Arg/Arg: OR= 0.96, 95%CI= 0.74 to 1.24 chi2= 0.06, p= 0.8). Moreover, no significant association was detected in variant allele (Pro vs Arg: OR= 1.075, 95%CI= 0.91 to 1.25), homozygous (Pro/Pro vs Arg/Arg: OR=0.911 95%CI= 0.66 to 1.25), and heterozygous (Arg/Pro vs Arg/Arg: OR= 0.84, 95%CI= 0.7 to 1). Conclusion: Our study revealed that p53 codon 72 Arg>Pro polymorphism was not associated with overall cancer odds in Iranian population.

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Association of Two Polymorphic Codons in P53 and ABCC1 Promoter with Prostate Cancer

Cited by 8 publications

References 34 publications

eIF5B regulates the expression of PD-L1 in prostate cancer cells by interacting with Wig1

eIF5B regulates the expression of PD-L1 in prostate cancer cells by interacting with Wig1

Application of intravoxel incoherent motion imaging in the study of migration and movement of cancer cells

Association of p53 codon 72 Arg>Pro polymorphism and risk of cancer in Iranian population: A systematic review and meta-analysis

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