Association of Vitamin B12, Lactate Dehydrogenase, and Regulation of NF-κB in the Mitigation of Sodium Arsenite-Induced ROS Generation in Uterine Tissue by Commercially Available Probiotics

Chattopadhyay, Sandip; Khatun, Shamima; Maity, Moulima; Jana, Suryashis; Perveen, Hasina; Dash, Moumita; Dey, Arindam; Jana, Lipi Rani; Maity, Pikash Pratim

doi:10.1007/s12602-017-9333-3

Cited by 14 publications

(5 citation statements)

References 103 publications

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“…The intracellular antioxidant enzymatic activity in female reproductive organs was reduced by the excessive ROS production caused by the arsenic intoxication. These results consistent with previous findings 24 . Electrozymographic impression revealed that, the treatment of AA (1.0 mg / 100gm body weight) and vitamin B 12 (0.09 μg / 100gm body weight) alone or jointly in arsenic ingested rats protected the uterine and ovarian antioxidant enzymatic activities (SOD, catalase, and GPx) due to its probable own intrinsic antioxidant properties Fig.…”

Section: Discussionsupporting

confidence: 94%

“…To identify the nature of the follicles the diameter of each follicle was measured and quantifies the number of follicles in different stages of folliculogenesis in each ovary 24 . The follicles were categorized as small preantral follicles (SPAF), large preantral follicles (LPAF), small antral follicles (SAF), medium antral follicles (MAF), large antral follicles (LAF), graafian follicles on the basis of their morphology and diameter and the numbers of regressive follicles (RF) were counted.…”

Section: Ovarian and Uterine Histopathologymentioning

confidence: 99%

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2019

IJPSR

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The present study was proposed to delineate the curative role of arjunolic acid and vitamin B 12 alone or in combination against arsenic induced oxidative stress and female reproductive dysfunction. The Wistar strain adult female rats were treated with sodium arsenite at the dose of 1.0 mg/100 gm body weight orally for first eight days. Next eight days rats were treated with arjunolic acid (1.0 mg/100 gm body weight) and vitamin B 12 (0.09 μg/100 gm body weight) orally. In ovarian and uterine tissue showed a recovery in arsenic induced ROS-production following the post-treatment of arjunolic acid and vitamin B 12. Antioxidant status was also improved by arjunolic acid and vitamin B 12 as established from the electrozymographic analysis of SOD, catalase and GPx. Post-treatment of these two significantly retrieved ovarian estradiol receptor 1 (ESR-1) from arsenic induced down regulation. Arjunolic acid and vitamin B 12 also showed its curative ability in improving the level of inflammatory markers such as NF-κB, metallothioneine-1 (MT-1), TNF-α, IL-6. Thus these two bioactive substances might have the curative role against arsenic induced female reproductive dysfunction. INTRODUCTION: The trioxide form of arsenic has the ability to react with cellular thiols to exert its toxicity and during the metabolism of thiols in cells reactive oxygen species generates which thereby promotes tissue damage 1. Arsenic is the cause of several reproductive hazards. It impairs spermatogenesis process and decreases the spermatogenetic enzymes in male reproductive system. The spermatogenic meiosis and the postmeiotic stages were altered by arsenic. Gonadal dysfunctions like declined testosterone synthesis, apoptotic and necrotic changes due to arsenic mediated oxidative damage 2 may cause male infertility.

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Section: Discussionsupporting

confidence: 94%

Section: Ovarian and Uterine Histopathologymentioning

confidence: 99%

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2019

IJPSR

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“…Since the production of reactive oxygen species is high in reproductive tissue due to active metabolism and steroidogenesis, the tissue is under continuous oxidative stress. The result of the present investigation demonstrated the adverse effect of arsenic treatment on female gonadal steroidogenesis and antioxidant defense mechanism 29 . In ovarian steroidogenesis 3 and 17 -HSDs are the key regulatory enzymes, the suppressed activities of these enzymes along with elevated cholesterol levels by arsenic suggest alteration in its hormone synthesis 27,30,31 .…”

Section: Resultsmentioning

confidence: 53%

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2019

IJPSR

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The present study was designed to determine the role of Andrographis paniculata (AP; 50 mg/kg) against the oxidative stress caused by the arsenic (0.5 and 1.0 mg/kg) for 30 days in adult mice. The results of in-vivo studies demonstrated that arsenic treatment resulted in a significant dose-dependent increase in the ovarian arsenic level and lipid peroxidation (LPO), followed by a marked reduction in gravimetric data, levels of total ascorbic acid, glutathione and in the activities of superoxide dismutase, catalase, and glutathione peroxidase. A significant reduction in the activities of 3 and 17 hydroxysteroid dehydrogenases along with elevated levels of cholesterol indicated ovarian endocrine dysfunction. These results suggest that an increase in free radical formation relative to loss of antioxidant defense system during arsenic exposure may render ovary more susceptible to oxidative damage leading to their functional inactivation. On the other hand, co-administration of arsenic with Andrographis paniculata (AP) restored altered biochemical variables, antioxidant balance, and gravimetry which suggests that the free radical-mediated damage during arsenic-induced oxidative stress could have been ameliorated by A. paniculata (AP) by its anti-oxidative potential.

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“…Administration of L. plantarum CCFM8610 reversed all of the reductions of mRNA expression of tight-junction proteins (ZO-1, ZO-2, occludin, and claudin-1) caused by Cd exposure, decreased intestinal permeability and reduced Cd leakage into systemic circulation [50]. Preventing systemic absorption of HMs by probiotics thus leads to alleviation of oxidative stress in various tissues and consequent mitigation of tissue damages as reported [100,101,102]. For example, co-treatment of L. plantarum CCFM8610 and Cd cause a decreased production of metallothionein and downregulation expression of genes in the mitogen-activated protein kinases (MAPK) pathways in the liver [19].…”

Section: Probiotics As a Potential Tool In Contaminants Remediationmentioning

confidence: 97%

A Review on Gut Remediation of Selected Environmental Contaminants: Possible Roles of Probiotics and Gut Microbiota

et al. 2018

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Various environmental contaminants including heavy metals, pesticides and antibiotics can contaminate food and water, leading to adverse effects on human health, such as inflammation, oxidative stress and intestinal disorder. Therefore, remediation of the toxicity of foodborne contaminants in human has become a primary concern. Some probiotic bacteria, mainly Lactobacilli, have received a great attention due to their ability to reduce the toxicity of several contaminants. For instance, Lactobacilli can reduce the accumulation and toxicity of selective heavy metals and pesticides in animal tissues by inhibiting intestinal absorption of contaminants and enhancing intestinal barrier function. Probiotics have also shown to decrease the risk of antibiotic-associated diarrhea possibly via competing and producing antagonistic compounds against pathogenic bacteria. Furthermore, probiotics can improve immune function by enhancing the gut microbiota mediated anti-inflammation. Thus, these probiotic bacteria are promising candidates for protecting body against foodborne contaminants-induced toxicity. Study on the mechanism of these beneficial bacterial strains during remediation processes and particularly their interaction with host gut microbiota is an active field of research. This review summarizes the current understanding of the remediation mechanisms of some probiotics and the combined effects of probiotics and gut microbiota on remediation of foodborne contaminants in vivo.

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Association of Vitamin B12, Lactate Dehydrogenase, and Regulation of NF-κB in the Mitigation of Sodium Arsenite-Induced ROS Generation in Uterine Tissue by Commercially Available Probiotics

Cited by 14 publications

References 103 publications

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A Review on Gut Remediation of Selected Environmental Contaminants: Possible Roles of Probiotics and Gut Microbiota

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