Association of vitamin D receptor gene polymorphisms with gestational diabetes mellitus-a case control study in Wuhan, China

Abstract

Background Gestational diabetes mellitus (GDM) increased risk of perinatal complications for both the women and the fetuses. The association between the vitamin D receptor (VDR) gene polymorphism and GDM has not been thoroughly investigated in Chinese pregnant women. Therefore, we aimed to determine whether VDR gene single nucleotide polymorphisms (SNPs) rs154410, rs7975232, rs731236, rs2228570 and rs739837 contribute to GDM risk in Wuhan, China. Moreover, we aimed to explore their combined effects on the risk of GDM. Methods Pregnant women who had prenatal examinations at 24 to 28 weeks’ gestation in our hospital from January 15, 2018 to March 31, 2019 were included in this case-control study. After exclusion, a total of 1684 pregnant women (826 GDM patients and 858 non-diabetic controls) were recruited. The clinical information and blood samples were collected by trained interviewers and nurses. Genotyping of candidate SNPs was conducted on the Sequenom MassARRAY platform. Statistical analyses including t-test, ANOVA, chi-square test and logistic regression were performed to the data with SPSS Software to evaluate differences in genotype distribution and associations with GDM risk. Multifactor dimensionality reduction method was used to explore the gene-gene interactions on the risk of GDM. Results Differences in age, pre-pregnancy BMI, family history of diabetes and previous history of GDM between the case and control groups were statistically significant (P < 0.05), whereas no significant differences were found in height, gravidity, parity, and age of menarche (P > 0.05). There were no significant differences at genotype distributions of the examined VDR gene SNPs (P > 0.05). After adjusting by age, pre-pregnancy BMI, family history of diabetes, the results of logistic regression analysis showed no associations of the five SNPs with GDM in all the four genotype models(P > 0.05). Furthermore, there were no gene-gene interactions on the GDM risk among the five examined VDR gene SNPs. Conclusions The VDR gene SNPs rs154410, rs7975232, rs731236, rs2228570 and rs739837 showed neither significant associations nor gene-gene interactions with GDM in Wuhan, China.

The Association Between FokI Vitamin D Receptor Polymorphisms With Metabolic Syndrome Among Pregnant Arab Women

“…In some epidemiologic studies, the shorter FokI VDR allelic variation has been associated with a host of adverse disease outcomes (16,17), including increased risk to MetS in adults (2,3,15,16,18,19). Furthermore, increased susceptibility to GDM from expectant mothers having the FokI variant were observed among expectant mothers coming from Turkey (20) and Iran (21), but not Chinese (22) and Saudi Arabians (23), which means that FokI may confer susceptibility only to select populations (24). To date, there is scarcity of evidence linking MetS and FokI VDR SNPs in pregnant women, more so in understudied ethnic groups such as Saudi Arabians.…”

The Significance of VDR Genetic Polymorphisms in the Etiology of Preeclampsia in Pregnant Polish Women

“…It has often been indicated that some VDR gene variants could be associated with an increased risk of chronic hypertension [ 49 , 50 ]. Thus far, several publications have analyzed the relationship of VDR gene polymorphisms with the occurrence of certain complications in pregnancy [ 27 ].…”

“…The results were corroborated by the research performed by Wang et al, in which the authors found associations of VDR BsmI and FokI variants with hypertension risk in Japanese men [ 49 ]. Additionally, a meta-analysis performed by Zhu et al revealed a significant correlation between the VDR BsmI variant and susceptibility to hypertension [ 50 ]. The very exciting result in our study was the observation that 3-marker haplotype CTA ( TaqI, ApaI, BsmI ) was associated with significantly higher systolic ( p = 0.0075) and diastolic ( p = 0.0072) blood pressure values.…”

Melatonin Receptor 1B Genetic Variants on Susceptibility to Gestational Diabetes Mellitus: A Hospital-Based Case–Control Study in Wuhan, Central China

“…The method of data collection was reported in previous article. 25 The study protocol was approved by the institutional review board of Wuhan University of Science and Technology and was performed in accordance with the Declaration of Helsinki. All subjects provided informed consents for participation.…”

“…The method of sample collection was also reported in the previous article. 25 The aforementioned five SNPs were genotyped on the Sequenom MassARRAY platform (Sequenom Inc., San Diego, CA, USA). For quality control: (i) case and control samples were mixed on each plate; (ii) genotyping was performed blinded to case or control status; (iii) two water controls were used in each plate as blank controls; (iv) 5% of the samples were randomly selected for repeated genotyping, as blind duplicates, and the reproducibility was 100%.…”