Association of vitamin D receptor gene polymorphisms with chronic and aggressive periodontitis in Jordanian patients

Abstract

Vitamin D acts through binding with vitamin D receptor (VDR) and is responsible for regulating bone metabolism and mineralization; it also suppresses the immune system. The aim of this study was to investigate if VDR gene polymorphisms are associated with chronic periodontitis (CP) and aggressive periodontitis (AgP) in a Jordanian population. A total of 99 patients with CP, 63 patients with AgP, and 126 controls were genotyped using PCR-restriction fragment length polymorphism (RFLP) for BsmI, ApaI, and TaqI single nucleotide polymorphisms (SNPs). The association was determined after correcting for confounding factors using multivariate logistic regression analysis. Estimation of haplotype frequencies was carried out using the EH program, and haplotypes were constructed using the phase 2.1 program. After correcting for confounding factors, multivariate logistic regression analysis revealed that inheritance of the BsmI bb genotype or the ApaI aa genotype was associated with increased risk of developing CP (OR = 2.4 and OR = 3.4, respectively) but with reduced risk of developing AgP (OR = 0.4 and OR = 0.3, respectively). This was further supported by association of the ba haplotype with CP but not with AgP. This study supports an association of VDR gene polymorphisms with CP and AgP in a Jordanian population; however, the pattern of association was different between the two diseases.

Vitamin-D receptor (VDR) gene polymorphisms (Taq-I & Apa-I) in Syrian healthy population

“…The allelic frequency of ‘T’ vs ‘t’ and ‘A’ vs ‘a’ were 65 vs 35% and 66 vs 34% respectively in Syrian population. Comparison of frequency distribution of different genotypes and alleles of VDR gene was done between Syrian and different populations that mentioned in North Indian study ( Bid et al, 2005 ), Turkey ( Gunes et al, 2008 ), Iran ( Nosratabadi et al, 2010 ) and Jordan ( Karasneh et al, 2013 ) using χ 2 tests. In this study, no differences in the distribution of genotypes and alleles of Taq-I were found between Syrian individuals and each of the populations of Jordan, Sweden, Black Pennsylvania and Australia as shown in Table 2 .…”

“…In this table, the data were adapted basically from the North Indian study ( Bid et al, 2005 ) and studies from Jordan (+) ( Karasneh et al, 2013 ), Turkey (+) ( Gunes et al, 2008 ) and Iran (+) ( Nosratabadi et al, 2010 ). Whereas the distribution of genotypes and alleles of Apa-I was similar in Syrians compared to populations of Jordan, North India, Turkey, France, Greece and Black Pennsylvania as shown in Table 3 .…”

“…Whereas the distribution of genotypes and alleles of Apa-I was similar in Syrians compared to populations of Jordan, North India, Turkey, France, Greece and Black Pennsylvania as shown in Table 3 . In this table, the data were adapted basically from the North Indian study ( Bid et al, 2005 ) and studies from Jordan (+) ( Karasneh et al, 2013 ), Turkey (+) ( Gunes et al, 2008 ) and Iran (+) ( Nosratabadi et al, 2010 ).…”

“…There are differences among different populations as presented in Table 2 , Table 3 . Syrian population was compared to all other different populations that mentioned in the North India study ( Bid et al, 2005 ) in addition to populations of Iran, Turkey and Jordan ( Karasneh et al, 2013 ), which are Asian countries.…”

Vitamin D and Its Relevance in the Etiopathogenesis of Oral Cavity Diseases

“…Inagaki et al (2003) and Naito et al (2007) reported that the AA genotype was associated with a higher risk of severe CP and with more advanced alveolar bone loss. However, Karasneh et al (2013) observed that CP occurred more frequently in subjects with AA genotype. In addition, studies of the impact of Bsml and Fokl polymorphisms on the development of periodontal inflammation have provided ambiguous results.…”