Association study of cell cycle proteins and human papillomavirus in laryngeal cancer in Chinese population

Abstract

Objective To investigate the role of human papillomavirus (HPV) in laryngeal squamous cell carcinoma (LSCC) and analyse the relationship between HPV and the expression of cell cycle‐related proteins. Design The study consisted of LSCC between 2005 and 2011 in Tongren Hospital. Clinical data such as age, sex, smoking/alcohol consumption, and TNM stage were collected. HPV DNA and cell cycle‐related proteins were assessed in terms of clinical features. Setting Single‐centre study. Participants A total of 332 LSCC patients were included in the study. Main outcome measures The presence of genotype‐specific HPV DNA was evaluated using PCR‐RDB in formalin‐fixed paraffin‐embedded tissues. All samples were also evaluated for p16INK4A, p21WAF1/CIP1, P53, Cyclin D1, and Ki67 immunohistochemical staining by tissue microarray. Results HPV DNA was detected in 45 of 332 (13.55%) patients with LSCC, with HPV‐16 being the predominant genotype. The presence of HPV‐16 DNA was significantly associated with basaloid squamous cell carcinoma and cystic lymph node metastasis (P < 0.05). Of the 332 patients, 36 (10.84%) were scored as p16INK4A positivity and they were more likely to be female (P < 0.05). Cyclin D1‐positivity and p21WAF1/CIP1‐positivity were observed in 60.24% (200/332) and 40.66% (135/332), respectively. In 114 cases (34.33%), LSCCs had moderate‐ to ‐strong p53 accumulation, which was correlated with TNM stage (P < 0.05). HPV‐16 DNA was correlated with p16INK4A and manifested a higher Ki‐67 labelling index and p21WAF1/CIP1 expression than HPV‐16‐negative tumours (P < 0.05). No relationship was observed between Cyclin D1or P53 expression and HPV‐16 infection (P > 0.05). Conclusion HPV DNA was detected in 13.55% patients with LSCC, with HPV‐16 being the predominant genotype and it was correlated with p16INK4A and manifested a higher Ki‐67 labelling index and p21WAF1/CIP1 expression than HPV‐16‐negative tumours.

Prevalence and Prognostic Value of HPV among Tunisian Patients with Laryngeal Cancer and Relationship between DNA HPV and p16, IGF-1R, Survivin, p53 Expressions

“…50,51 In our series, this association between the HPV status and p53 expression was not found consistent with other studies. 52,53 On the other hand, the association between p16 expression and the presence of HPV DNA was not found in the laryngeal tumors of our series, which is in agreement with results already published. 54 Moreover, this relationship has not been detected in the study of the relationship between the HPV transcriptional activity and the p16 expression.…”

“…50,51 In our series, this association between the HPV status and p53 expression was not found consistent with other studies. 52,53…”

Bioinformatics analysis of laryngeal squamous cell carcinoma: seeking key candidate genes and pathways

“…The PI3K-Akt signalling pathway has a vital function in LSCC by suppressing cell death (Chrysovergis et al, 2019;Yang et al, 2019). P53, a tumour suppressor factor, initiates DNA repair, cell cycle arrest and apoptosis and reacts to various kinds of cancer therapies (Cui, Qu & Liu, 2019;Ragos et al, 2018). The changes above are connected with the findings in this study that the BPs of the DEGs necessarily contribute to the progression of LSCC.…”

Correlation between HPV PCNA, p16, and p21 Expression in Lung Cancer Patients

“…Since HPV requires the host cell DNA replication machinery for its progeny production, the virus must ensure that the S-phase of the cell cycle continues, and to do so, several key molecules suffer alteration, which is a fact in the role of HPV in cervical cancer development. In nonanogenital HPV-related tumors, such as head and neck cancers (HNC), it was already observed that cyclin-dependent kinase inhibitors (CKI) p16 and p21 are two of the disrupted factors [7,8] together with the proliferating cell nuclear antigen (PCNA) in order to keep the cell in constant proliferation [9].…”