Association Study of Symbiotic Genes in Pea (Pisum sativum L.) Cultivars Grown in Symbiotic Conditions

Zhukov, Vladimir A.; Zhernakov, A. I.; Sulima, Anton S.; Kulaeva, Olga A.; Kliukova, Marina S.; Afonin, Alexey М.; Shtark, O. Y.; Тихонович, И. А.

doi:10.3390/agronomy11112368

Cited by 6 publications

(3 citation statements)

References 55 publications

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“…Plant material consisted of two pea ( Pisum sativum L.) cultivars, ‘Classic’ (The Netherlands) and ‘Vendevil’ (France; = k-8274 in VIR catalog), as well as breeding line ‘Triumph’ obtained by the five backcrosses of the aforementioned cultivars [ 18 , 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…In accordance with this proposal, pea genotypes with high symbiotic responsivity have been identified in the germplasm collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) [ 17 , 18 ] and subsequently involved in breeding programs. As a result of backcrossing of cultivar ‘Classic’ (a donor of plant architectonics) on cultivar ‘Vendevil’ (=k-8274 in the VIR collection, a donor of symbiotic responsivity), the pea breeding line with increased EIBSM named ‘Triumph’ has been developed [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Genomic and Transcriptomic Analysis of Pea (Pisum sativum L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity

Zorin,

Sulima,

Zhernakov

et al. 2023

Plants

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Pea (Pisum sativum L.), like most legumes, forms mutualistic symbioses with nodule bacteria and arbuscular mycorrhizal (AM) fungi. The positive effect of inoculation is partially determined by the plant genotype; thus, pea varieties with high and low symbiotic responsivity have been described, but the molecular genetic basis of this trait remains unknown. Here, we compare the symbiotically responsive breeding line ‘Triumph’ of grain pea with its parental cultivars ‘Vendevil’ (a donor of high symbiotic responsivity) and ‘Classic’ (a donor of agriculturally valuable traits) using genome and transcriptome sequencing. We show that ‘Triumph’ inherited one-fourth of its genome from ‘Vendevil’, including the genes related to AM and nodule formation, and reveal that under combined inoculation with nodule bacteria and AM fungi, ‘Triumph’ and ‘Vendevil’, in contrast to ‘Classic’, demonstrate similar up-regulation of the genes related to solute transport, hormonal regulation and flavonoid biosynthesis in their roots. We also identify the gene PsGLP2, whose expression pattern distinguishing ‘Triumph’ and ‘Vendevil’ from ‘Classic’ correlates with difference within the promoter region sequence, making it a promising marker for the symbiotic responsivity trait. The results of this study may be helpful for future molecular breeding programs aimed at creation of symbiotically responsive cultivars of pea.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genomic and Transcriptomic Analysis of Pea (Pisum sativum L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity

Zorin,

Sulima,

Zhernakov

et al. 2023

Plants

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“…For a long time, the main objectives of pea breeding focused on improving root rot and powdery mildew control, according to Fondevilla and Rubiales [18] and Zhukov et al [19]. Enhancing pea cultivars with improved yield and quality features is necessary due to the economic strength of the pea.…”

Section: Introductionmentioning

confidence: 99%

Yield Adaptability and Stability in Field Pea Genotypes Using AMMI, GGE, and GYT Biplot Analyses

Yang,

Soliman,

et al. 2023

Agriculture

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Pea (Pisum sativum L.) is a vital leguminous crop farmed worldwide. Pea plays an essential role in China’s crop rotation system, but the major restrictions to its cultivation are stability and low yield. Breeding for promising cultivars with a significantly high yield will impact the sustainability of pea production. Additionally, diverse environment trials are crucial in determining the best genotype. The new cultivar “Yunwan 52” was developed by hybridization and subsequently evaluated through yield trials among six pea genotypes across 14 environments during the 2016–2018 growing seasons. The results showed that the average yield of “Yunwan 52” for all tested environments was 2.64 t ha−1 compared to the control cultivar (Yunwan 18, 1.83 t ha−1). Analysis of AMMI variance showed significant differences (p < 0.01) between genotypes, environments, and their interaction. Based on the GGE biplot, some genotypes possessed wide and narrow adaptability to environments, such as Yunwan 52 was considered the most stable and ideal gen-otype across all tested environments. GYT biplot analysis also revealed that this realized cultivar was a superior and stable genotype that can be identified visually by combining all characters in breeding programs. Yunwan 52 distinguishes with purple blossoms and seed coat peas. It is possible to infer that the newly released cultivar “Yunwan 52” has outstanding yield performance and wide adaptability to multiple environmental conditions (resilience to abiotic stress). It will contribute to developing nutritional pea genotypes and increase pea production in irrigated areas.

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Diversity of PsSym29 and PsNRLK1 genes in the VIR germplasm collection of pea (Pisum sativum L.)

Zhukov

Zhernakov²,

Belozerova³

et al. 2022

Ecological genetics

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BACKGROUND: N.I. Vavilov Institute of Plant Genetic Resources (VIR) (Saint Petersburg, Russia) maintains a large collection of pea (Pisum sativum L.). Earlier, several growth and yield parameters were recorded for plants of 99 accessions grown under inoculation with nodule bacteria and arbuscular mycorrhizal fungi. MATERIALS AND METHODS: Polymorphism of genes encoding symbiotic receptor kinase PsSym29 [participating in the autoregulation of nodulation (AON) system] and closely related receptor kinase PsNRLK1 (with yet unknown function in symbiosis) was assessed in 99 pea genotypes from the VIR collection. Nucleotide diversity, Tajimas D, and Fay and Wus H statistics were calculated using DNAsp 5.0 software. The significance of associations of allelic state of the sequenced genes with the growth and yield parameters was tested by two-way ANOVA followed by FDR correction and by regression analysis. RESULTS: Nucleotide diversity and the ratio of synonymous to non-synonymous substitutions was greater in PsNRLK1 as compared to PsSym29. The analysis of Fay and Wus H in sliding window revealed signatures of positive selection in one site of PsSym29 and in three sites of PsNRLK1 gene sequences located in 1st exons encoding LRR (leucine rich repeat) domains. No significant associations of allelic state of neither PsSym29 nor PsNRLK1 genes was found with plant growth and yield parameters. CONCLUSIONS: The sequences of both PsSym29 and PsNRLK1 genes undergo positive selection, but the conditions in which specific allelic states of the genes become adaptive are to be elucidated in future.

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Association Study of Symbiotic Genes in Pea (Pisum sativum L.) Cultivars Grown in Symbiotic Conditions

Cited by 6 publications

References 55 publications

Genomic and Transcriptomic Analysis of Pea (Pisum sativum L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity

Genomic and Transcriptomic Analysis of Pea (Pisum sativum L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity

Yield Adaptability and Stability in Field Pea Genotypes Using AMMI, GGE, and GYT Biplot Analyses

Diversity of <i>PsSym29</i> and <i>PsNRLK1</i> genes in the VIR germplasm collection of pea (<i>Pisum sativum</i> L.)

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